We narrowed to 695 results for: prot

...crisis. I like how they are using protocols.io to create a protocol community! Asclepias syriaca and Asclepias...solicitation requires funded labs to rapidly disseminate protocols, methods, data, and tools that result from their...manipulated by injected RNA and can express fluorescent proteins. This lab has created an automated tank for feeding... fusion is such that the two plants share RNA, proteins, and DNA. Euprymna scolopes (Joshua Rosenthal...the brain allows the squid to create a different protein. Did you know that most cephalopods die after they...

...antibiotics also block protein synthesis, and the neomycin resistance gene protects against any of them....and eukaryotes. For example, puromycin inhibits protein synthesis across kingdoms by targeting a shared...A puromycin resistance gene on your vector can protect both plasmid-carrying bacteria and eukaryotes. ... vector with a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters might...production, you probably don’t need it to express your protein of interest. However, you do still need a species-compatible...

... target is present immediately upstream of a Protospacer Adjacent Motif (PAM), the sequence NGG.  If these...site e.g., deleting the catalytic residue of a protein or knocking out a very small peptide. In these ... within the editing window (bases 4-8 of the protospacer). While this is a small window, this system can... can be used with a variety of impaired Cas9 proteins (such as SpRY) with altered PAM requirements, giving...epitope tagged dCas9 and antibody-activator fusion proteins. The Synergistic Activation Mediator complex, ... target sequence has been modified!  The exact protocol may vary depending on your specific experiment...

...major structural protein GvpA, which seemed overexpressed compared to accessory proteins. Excess gvpA plasmid...of ARGs results in the production of air-filled protein nanostructures called gas vesicles, which scatter.... coli. We deleted an unannotated hypothetical protein, improving expression, and named this gene set ...of toxicity or burden relative to a fluorescent protein control. We also added the toxin-antitoxin stability...in mammalian cells without the shell stiffening protein GvpC, which allowed the GVs to be flexible and ...

...the Gateway System for Protein Expression in Multiple Hosts. Curr Protoc Protein Sci. 2003 Feb;Chapter ... during the BP or LR reactions. Since the CcdB protein inhibits the growth of CcdB sensitive E. coli strains...strategy, it’s important to include the proper protein expression elements (ribosome recognition sequences...Resources on Addgene.org Find Plasmid Cloning Protocols Watch Our Diagnostic Restriction Digest Video ...

...triggers. This is done by creating a split dCas9 protein that can reassemble when exposed to specific UV...similar tools were made using other DNA binding proteins such as zinc fingers (ZF) and TAL effectors (TALE...new gRNA rather than engineering an entirely new protein. This makes it much cheaper to use Cas9 activators...CRISPR-based screens can be used to gain insight into protein structure and function (Pan et al., 2018). cDNA... A, Kadoch C (2018) Interrogation of Mammalian Protein Complex Structure, Function, and Membership Using...Gilbert LA, Qi LS, Weissman JS, Vale RD (2014) A Protein-Tagging System for Signal Amplification in Gene...

...for a lentivirus are determined by the envelop protein. Many times lentiviral vectors are engineered with...Lentiviruses pseudotyped with the VSV-G envelope protein use the LDL receptor to infect cells and have a...However, viruses pseudotyped with other envelope proteins may have a limited number of targets and therefore...in the mix Even before the lentiviral envelope protein attaches to cell surface receptors, other important...virus properly and be consistent when following protocols. Need Virus? Check out Addgene's New Viral Service...pseudotyped HIV-1-based lentiviral vectors."Nature protocols 4.4 (2009): 495-505. PubMed PMID: 19300443. 7....

... be used as a proxy measure for differences in protein expression. (If you're just getting started, check...included in commercial sets and most machines have a protocol that automatically performs the normalization ...normalization step.  Machines Most general-use protocols, unless otherwise specified, were likely developed on the...LightCycler, you’ll likely need to re-optimize protocols for that machine. (Teo, et al, 2002; see paper...an individually run sample when developing the protocol and optimized until the multiplexed and individual...samples or sample conditions change, the multiplexed protocol may have to be re-optimized.  While troublesome...eds.), Quantitative Real-Time PCR: Methods and Protocols, Methods in Molecular Biology, vol. 1160, DOI ...

...envelope protein under the control of the powerful mammalian CMV promoter. Envelope proteins are a key...key component of lentiviruses. These proteins coat the virus and help determine the types of cells that... preps. You can find our lentivirus production protocol on our website and tips for titering your lentivirus...check out our lentivirus guide page. For more protocols and information, be sure to check out the Trono...

...production standpoint, it wasn’t ideal for our Lab Protocol videos. It’s really nice to have a scientist on-screen... on-screen, somebody who really knows the protocol and can guide viewers through. I know cameras, but ...thankfully volunteered, and we decided to focus on a protocol that didn’t require any lab shooting, just animation...setup for the lab, so we can get back to producing protocol videos?   Figure 4: The remote directing...the pandemic with some incredibly strict safety protocols to keep Addgenies safe. Space was at a premium...why not check out all of the written and video protocols at Addgene? If you need help in the lab, we have...

...at the Institute for Protein Innovation (IPI), a Boston-based, nonprofit protein research institute, partnered...containing ligand-binding heads and long legs that protrude from the cell membrane, physically connecting ...Table 1: IPI antibodies  Antibody Target Proteins Reactivity Isotype Source Species Anti-Integrin...plasmids. To encourage collaboration and education in protein science, IPI hosts events and training courses ...Faulds is a science writer at the Institute for Protein Innovation. She writes about all things IPI, from...

...plasmids Anti-CRISPR proteins for optogenetic control of CRISPR-Cas9 Anti-CRISPR proteins that inhibit type...Find the plant genome editing toolkit New Cas protein identified Jennifer Doudna’s lab recently identified...identified Cas14, a family of small DNA-targeting Cas proteins. Cas14 targets and cleaves ssDNA which they have...Now Dominik Niopek’s lab has adapted anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. By combining...

... ortholog was fused to a different fluorescent protein to create three colors. The specificity of these...estimate that a minimum of 150-200 fluorescent protein molecules is necessary for a detectable signal,... scaffold that can be used to recruit multiple protein molecules (and also available from Addgene!) Since...plasmids from this publication at Addgene. A protein-tagging system for signal amplification in gene... fluorescent protein...

...discovery of microbial rhodopsins, light-sensitive proteins that absorb photons for energy conversion or the... et al., 1984; Harz and Hegemann, 1991). These proteins control the flow of electric charges across cell...Purification and properties of the Cre recombinase protein." Journal of Biological Chemistry 259.3 (1984):...Dieter, and Walther Stoeckenius. "Rhodopsin-like protein from the purple membrane of Halobacterium halobium...modification technology in the mouse." Current protocols in immunology 105.1 (2014): 10-34. PubMed PMID...clinical applications of optogenetics." Current protocols in pharmacology 75.1 (2016): 11-19. PubMed PMID...

...imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent protein biosensors ...ARGs) are engineered gene clusters that encode proteins that form bacterial gas vesicles. These ~200nm...200nm long vesicles have hollow interiors and a protein shell that excludes water but is gas permeable. These...luciferase reporter gene The green fluorescent protein reporter gene Resources on Addgene.org Find plasmids...

...re-exposure (2, 3). More specifically, specialized Cas proteins snip the foreign DNA into small fragments approximately...paste them into the CRISPR sequence. Separate Cas proteins then express and process the CRISPR loci to generate...genetic material next to the species-specific protospacer adjacent motif (PAM). Once the CRISPR/Cas complex...out these functions using slightly different Cas proteins and PAM sequences. A total of eight evolutionarily...the CRISPR/CAS toolkit by posting generic lab protocols, providing tips from experts in the field, and...

...introductory blog post, then check out this RNP protocol on Protocols.io. The PAM Requirement and Expanding CRISPR...biophysics can help enhance HDR. CRISPR 101: Ribonucleoprotein (RNP) Delivery The RNP delivery method consistently...Channabasavaiah B. Gurumurthy, and Masato Ohtsuka. Nat Protoc. 13(1) (2018):195-215. PubMed PMID: 29266098 Creating...models with CRISPR has been challenging, with protocols using dsDNA templates achieving ~1-10% efficiency...

...antiviral defense) complexes, while Class 2 use single protein Cas effectors, like Cas9. The Cascade complex doesn...effector complex but are defined by the accessory Cas proteins in the Cascade complex. Despite a tenuous grasp... Despite being in Class 1, Cas7-11 is a single protein effector and is able to target and edit RNA in ...Class 2 Class 2 CRISPR systems rely on a single protein Cas effector and are likely the class most people...discovery algorithms find new candidates for CRISPR proteins. A type VII is already on the horizon (Altae-Tran...2021). Programmable RNA targeting with the single-protein CRISPR effector Cas7-11. Nature, 597(7878), 720...

...acids (DNA and RNA) absorb maximally at 260 nm. Proteins on the other hand absorb best at 280 nm and organic...can’t distinguish between DNA and RNA. See our protocol about using UV absorbance to quantify DNA Fluorescence...this method doesn’t tell you about contaminating proteins or chaotropic salts. Capillary electrophoresis...Addgene blog Read more about molecular biology protocols and tips Find the best way to elute and store ...Reference Find plasmids for your research Browse protocols for molecular biology, cloning, and more ...

...The catalytically dead Cas9 protein (dCas9) is well known for its ability to bind DNA targets without.... To do so, they fused a different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9...boxB) that each recruits a different fluorescent protein (BFP, GFP, or RFP) to the genomic loci of interest...) showing recruitment of different fluorescent proteins. (B) CRISPR-Sirius used to visualize relative ...Download our CRISPR eBook Learn about fluorescent protein-based tools Read all CRISPR blog posts Resources...