We narrowed to 842 results for: Eras

...needed for optogenetics. There are several important considerations with fabrication of the fiber optics...pulse generator such as the A.M.P.I Master-9 Pulse Stimulator, which can control output of several lasers...equipment, most of which is relatively cheap). Several protocols have been written outlining the different...stimulation. The scatter effect is also an important consideration when preparing the implant itself, which brings...least 85% of the initial laser output. The same considerations apply to the polishing and preparation of the... channel, Channelrhodopsin 2 (ChR2), exists in several states: closed/light receptive, open, closed/light...manipulation of the laser power source itself. Pulse generation (TTL pulse) is a standard electronics protocol...

... plasmids for generating your own libraries are also available in the Sybody Generation Toolbox plasmid... two different coronaviruses into a llama over several days and isolated special camelid antibodies that...proteins that may unfold due to camelid high body temperature (37.2 - 39.2°C). The Seeger lab found a way around... around this by using plasmids to generate sybodies, synthetic nanobodies against the spike protein (Walter...blog post.  A rapid selection platform for generating sybodies against membrane proteins Developing...membrane protein of interest using the Sybody Generation Toolbox and commercially available reagents in...libraries (Zimmermann et al., 2018). Find the Sybody Generation Toolbox! Starting with libraries and a biotinylated...

...yeast and cell free expression systems. Overall the general strategy for protein expression consists ...or purify proteins to study their structure, interactions and functions. Proteins can also be purified...purified for use in molecular biology research (eg. polymerases and other enzymes might be purified and used ...derived from cells or using live cells. There are several types of expression systems used for protein production...Deciding which expression system to use depends on several factors: The protein you are trying to express...most popular hosts for protein expression with several strains that are specialized for protein expression...be purified from the cellular debris. There are several popular DNA vectors that can be used to produce...

...GFP/EGFP) for a transcriptional reporter or as a general cytoplasmic label of cells, there's no problem....to not produce artifacts in your experiments. Considerable effort has gone into making FPs monomeric, yet...ignorant of FP dimerization. Equally problematic, several reportedly monomeric FPs are not actually monomeric...more likely molecules of a particular type will interact. A protein with a nanomolar Kd will exist primarily... of the FP. The FP is confined to a plane and generally only rotates in 360°, increasing the probability...2]. 2. Fusion to an obligate dimer or oligomer Several cell proteins normally self-associate into homodimers... and increasing the signal from the second FP. Several early FRET biosensors were made with CFP and YFP...

...Newman-Keuls method – More powerful. Moderate false negatives, moderate false positives. Duncan’s multiple...is being experimentally manipulated. Below are several core factors of statistics relevant for many downstream...Parametric tests  Statistical tests fall into two general categories: parametric and non-parametric. In this...variable. A two-way ANOVA could test the effect of temperature and humidity on an outcome such as total crop... one-way ANOVA can test either the effect of temperature or humidity on crop production.   MANOVA The ...example, a one-way MANOVA could test the effect of temperature on total crop production and the production window...A two-way MANOVA could measure the effect of temperature and humidity on total crop production and the...

...We often think of DNA as inert. It generally stays put, which makes it easy to locate in a genome. But...mobile DNA, called a transposon, that’s a bit hyperactive and likes to jump around from one location in... at rates similar to retroviral vectors. The hyperactive version of the SB transposase, SB100X, is ~100...100-fold more efficient compared to first generation SB transposase (Mátés et al., 2009). hySB100x improves...human cells similar to retroviral vectors. The hyperactive PB transposase (hyPB) is ~10-fold more active...transposon insertion sites are detected with next generation sequencing, and analyzed to identify which insertions...Transgenic animals Transgenic animals are often generated by directly injecting DNA into the pronuclei of...

...translation by generating a frameshift mutation. The majority of cellular repair events will generate these types... a less frequent DNA repair mechanism in general. On average, it accounts for less than 10% of DNA repair...family member which generates sticky ends upon cutting. Cas12a may promote HDR by generating ssDNA overhangs...Taking the road less traveled and generating a knock-in cell line instead of a knock-out? We’ve got you...editing Targeted genome editing events fall into two general classes: knock-in and knock-out. The goal of a ...exogenous DNA can be used as well. Below are some considerations for designing donor DNA for successful HDR ...viability of your cells. Enrich S-phase cells HR operates predominantly in S and G2 phase of the cell cycle...

... the notation works? The + operator denotes intersection and the – operator denotes set difference. That... ChiRP-seq RNA Hi-C DNA (genome-to-genome interactions) DNase-seq DNA (regions that are accessible... the foundation of modern drug discovery and therapeutics. We’ll have more to say about clinical applications...Poisson statistics, each sub-population has, on average, one distinct enhancer disrupted. As our ESCs die...differentiate over time, we periodically use next-generation sequencing to measure the relative proportion...apply this knowledge to create targeted disease therapies with minimal side-effects on healthy cells and...Hypersensitive Sites and Intergenic CRISPRs Yours truly generated the DHS data set by starting with all of the DHS...

...A7: A few notes below are considerations for designing HR donor. Generally if off-target (non-specific... and surveyor. Q15: I used DNA polymerase Takara Ex Taq™ DNA Polymerase for my genomic PCR, but couldn't...In our hands, Herculase II Fusion polymerase or Kapa Hifi Polymerase work very well. Some groups have ...concentration of Cas9 and guide RNA (gRNA). These considerations only matter if the PAM sequence is immediately...additional PAM sequences should not be sufficient to generate off-target DSBs; there needs to be extensive homology...most efficient and easiest way is to co-transfect several plasmid together, with each plasmid having a targeting...purified oligo is fine based on new data). For general transfection, you could use 1 µl of 10 µM oligo...

... are typically DNA preps, but we do distribute several of our most popular CRISPR pooled libraries and... A) Amplification, validation, and lentiviral generation of a pooled library. An aliquot of the pooled...experiments. Pooled library lentiviral preps are generally aliquoted to reduce the number of freeze-thaw ...libraries we amplify in house, but these are not general amplification protocols. Rather, each protocol ...amplified, you'll need to verify your library by next-generation sequencing (NGS). The representation of your ...our protocol on Lentivirus Production provides a general approach for producing lentivirus in Lenti-X 293T...you can transduce your cells.  Titering After generating your lentivirus, you’ll want to infect your cells...