...This post was originally published on the Quilt Genomics Blog and is republished here with permission. Quilt...enhancers, check out the Appendix. Conclusion: Shedding light on dark matter Precise knowledge of which...identified 23-mer gRNA. Off-target determination was established with Bowtie2 using the parameters first described...

...centrifugation or a magnet. 4) Unbound proteins are washed away. 5) The target protein is eluted.     ...and as in the first method, unbound proteins are washed away and the target protein eluted.  Immobilization...providing a solid support while unbound proteins are washed away.  While both Protein A and Protein G have ...because they do not require centrifugation between washes which can cause loss of beads, break weak protein...

... to only do the dishes once a month, resulting in a dangerous mountain of dirty dishes or you could choose...choose to do the dishes after every meal and be reassured that your dirty dishes won’t tumble out of the...

...chamber is divided into two halves that are distinguished visually through different patterns and the ...recently studied in detail thanks to optogenetics. In Goshen et al, inhibition of the CA1 region using HR was...your own neuroscience sub-discipline that has published using the technique and ask if they would be willing... opsins." Nature methods 9.2 (2012): 159-172.5. Goshen I, et al. Dynamics of retrieval strategies for ...

...factors OCT3/4, SOX2, KLF4, and c-MYC is the established model for reprogramming somatic cells. A common...Polycistronic cassettes that contained KLF4s had overall diminished KLF4 expression levels; this altered stoichiometry...as inhibitory GABA-ergic interneurons, Gordon Fishell’s lab has developed a new AAV approach using regulatory...to Choosing the B(right)est Fluorescent Protein. Shen Y, et al. PLoS ONE. 2017. PubMed PMID: 28241009 ...independent of another TTAGGG binding complex, shelterin. Swapping the DNA binding domain of another telomeric...

...publications and funding. If any of these three pillars crashes, the lab's success and the careers of its researchers...not enough. A 2013 study showed that out of 238 published scientific articles, only 46% could be reproduced...adopting simple note-taking tools, others using excel sheets or organizing folders in complex ways, there are...everything in one place. From pictures to excel sheets, researchers today generate vast amounts of digital...

... Additional resources ThermoFisher Scientific: Biotinylation ThermoFisher Scientific: Biotinylation...more information on ABC and IHC check out Thermo Fisher Scientific's guide. There are an array of other...which biotinylation reagent to use check out Thermo Fisher Scientific's Biotinylation Reagent Selection Tool...

...cytoskeleton and cell membrane dynamics. First, a quick refresher.  The tools below use the following optogenetic...reversible. Late last year, the Akhmanova Lab published a new optogenetic tool for localized microtubule...the membrane itself? The Baskin Lab previously published OptoPLD, an optogenetically controlled phospholipase...available for controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-CIB, and other optogenetic...

...sequencing or other readout. Cellular memory is accomplished by the use of sequence-specific enzymes (e.g...also available, N1-LOC8R.  These plasmids are unpublished Unnatural amino acid incorporation in mammalian...detection of neuronal action potentials. The Cohen lab wished to improve on the Arch indicator and their collaborators... that their sgRNA-RNA fusions were functional, Shechner et al used the constructs in their study to target...contiguous with the sgRNA core (see image above). Shechner, et al. Nat Methods. 2015. PubMed PMID: 26030444...regulation in live cells since their introduction by Sherf and Woods in the nineties. However, reliance on ...guess what? pXPG is now available at Addgene. Sherf, et al. 1994. Promega Notes Mag. Giannakis, et...

...system (PNS). (Capsids, if you recall, are the outer shell of an AAV vector, which determine the vector’s tropism...Addgene’s Data Hub and the CLOVER repository of published systemic AAVs to find commonly used vector cargo...capsid, while Table 1 gives a brief overview of published descriptions of commonly used or emerging capsids... plasmids available from Addgene Capsid Published Tropism Model Organism Mouse Strain-Specific...Caltech’s CLOVER Center’s extensive repository of published systemic AAV experiments and Addgene’s AAV Data...your validation data to our Data Hub once you’re finished!) Good luck and happy transducing! This post was...