We narrowed to 432 results for: sequence

...adding long-read sequencing capabilities to complement our next-generation sequencing, setting new standards... We’re also building a portal to easily share sequences and characterization data for over 150,000 plasmids...

...containing the modifying sequence flanked by approximately 50 base pairs of sequence homologous to the chromosomal... typically contain both a protein modification sequence and a selectable gene product for isolation of...

... reaction for traditional restriction cloning. Sequence and Ligation Independent Cloning (SLIC) could ...Invitrogen and based on lambda phage integration sequences, relies on site-specific recombination reactions...components and define their final order through sequence homology. Similarly, Golden Gate assembly allows...FastCloning: a highly simplified, purification-free, sequence-and ligation-independent PCR cloning method." ...

... the exact original sequence, it can restore the original protein-coding sequence using a recoding strategy...synthetic sequence will only spread in transgenic laboratory populations with that sequence2. Both methods...; a subsequent drive can restore the wild-type sequence (save for the residual sgRNAs and possibly cas9...unpredictable ecological effects. Because the consequences of such a mistake would necessarily extend far...

...immunoprecipitation followed by high-throughput sequencing, ChIP-Seq, is the go-to method for mapping where...noise, reducing the read depth necessary when sequencing and lowering the amount of tissue needed per ...subsequent library preparation and high-throughput sequencing. Improvements to the CUT&RUN method and using...

...fluorescence, like in the pMuSIC library, or by sequencing the unique DNA barcodes. Figure 1: Schematic...libraries accounts for chromatin, position, and sequence features in the selection of sgRNAs. Other CRISPR...library consists of broad host range regulatory sequences ready to be assembled with other elements using...

...Transposons are sequences of DNA that can move around in a genome. In a laboratory setting, transposons...the tranposon to integrate. Additionally, the TA sequence is duplicated near the gene of interest insertion...between a greater number of extinct transposon sequences and then testing various combinations of changes...

...?  scFv sequences have traditionally been cloned by amplifying the VLand VH antibody sequences of hybridomas...semi-synthetic or entirely synthetic VL and VH sequences. Phage display also allows for screening scFv ...antibodies. To do this, antibody variable domain sequences are expressed as scFvs and screened with in vitro...assays to select for strong binders. Then the sequences for the variable domains of the selected scFvs...

... generating insertions or deletions in the DNA sequence. This same approach is effective to target short...very small deletions and insertions in the DNA sequence can completely disrupt expression of the transcribed...Cas9 generates double-strand breaks, a new DNA sequence can be knocked-in, or introduced, into the break...off-target effects since promoter and enhancer sequences remain intact (Engreitz et al. 2019). The interactions...

...The raw data is then normalized to a reference sequence present in the same amount in all samples (usually...to run multiplex assays, allowing the reference sequence and the gene of interest to be amplified in the...for both the gene of interest and the reference sequence. At the end of the run, each droplet is read as...Complexity of the gene of interest Some gene sequences are harder to amplify and quantify than others...