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We narrowed to 885 results for: ANT;

Showing: 511 - 540 of 885 results

  1. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...plexWell™’s sequential tagging procedure, the quantities of library molecules for each sample remain highly...plate) will typically have a wide range of input quantities. This variation is problematic for conventional... individual libraries can be influenced by the quantity or quality of input samples. To minimize these...from multiple samples will then also need to be quantified and re-normalized in order to be combined with...proprietary chemistry). This turns out to be critically important for getting adequate sequencing depth and coverage...

  2. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...growing the cells on solid plates, where each transformant forms its own colony, uninfluenced by the growth... from both. These products are then purified, quantified, and sent for sequencing. Analyzing your sequencing...corresponding sequencing quality. Generally, you want to have 100x-1000x coverage of your library: in ...very similar to each other - that’s exactly what we want! At the end of the day, it’s up to you to decide...hope this post has helped clarify some of the important considerations when using, amplifying, and verifying...

  3. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ... of being honest and straightforward, it’s an important part of being a “good scientific citizen” and ...hurdle in front of publication when all they really want to do is get the paper off their desk and move on...only a partial solution.  How do we get where we want to be? The part that is still missing is a clear...management plans and for evidence of sharing for grant renewal—but there is clearly room to do more. If.... Sounds like a win for everyone. If we agree we want to get there, that’s half the battle. Many thanks...

  4. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...great for lab life. These apps prevent you from constantly searching the interwebs for lab resources, and... Lab Archives by LabArchives The single, most important part of lab life is the lab notebook. Lab Archives...always comes first with research! It is always important to know what kind of reagents you work with in...MSDS for a lab chemical of choice. Now, I can’t guarantee every reagent in your chemical closet is in this...iGreenEarth AMINO is a very simple app that packs important biological information, amino acid abbreviations...

  5. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ... active variant, the phage is not infectious. The editing speed dictates which phage variants can propagate...complete ABE protein. If the phage delivers an active variant of the base editor, a functional gene III product...stringency PACE, resulted in the evolution of TadA variants with much higher base editing activity compared... TadA-7.10 enzyme. After testing numerous TadA variants, they identified TadA-8e, which edits 590-fold...

  6. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...however, did not improve at the same pace as the quantity and quality of the information. As a result, resources...different strategies for finding what they need, and we wanted to support all of them — searching, browsing, drilling... supported and combined?  Luckily, we had the advantage of a very specialized domain of information so...search engine to return only the filters that are relevant to a specific query and category. For example,...that make our new search engine unique. Most importantly, with this new implementation, we laid the foundations...

  7. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...Harvard. Roo wants to simplify access to academic resources among economically disadvantaged populations...graduate students. In order to gain a competitive advantage this cycle, consider the advice below:  1. Eligibility... and lighting for a smooth performance. Most importantly, make certain that everyone you live with is ...increase your competitive edge, they alone, are no guarantee of success. Don’t luck into an internship, prepare...

  8. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ...most important resource that Addgene provides the scientific community? Katz: The most important resource...beyond. In your perspective, what do you think are important considerations for the future of open science?... on average 20 PhD’s a year and we have a very dominant presence in the biotech sector in both patent ...from Babson College. He is a certified public accountant (CPA), certified information systems auditor ...

  9. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...in low penetrant phenotypes that are hard to study. To overcome this, most scientists use mutant animals...leads to significantly stronger phenotypes than standard RNAi protocols without the need for mutant genetic...norovirus is enriched with synbodies--or synthetic antibodies--attached to magnetic beads, allowing the virus...

  10. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...several advantages over traditional vaccines, including 1) cellular responses in addition to antibody response...but this step can be engineered to be anything you want delivered to your target cells. This delivered DNA...the target cell or transiently expressed. It’s important to note that for all the applications we discuss...Gene expression control Researchers are always wanting to manipulate gene expression – turn it up, turn...

  11. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ... autophagy and, in a mouse model led to improved anti-cancer immunosurveillance and reduced tumor mass...on novel cancer therapeutics such as  (Chimeric Antigen Receptors) CAR T cells and PD-1 modulators. An ... et al. "Caloric restriction mimetics enhance anticancer immunosurveillance." Cancer cell 30.1 (2016):...PubMed PMID: 27411589. 2. Todoric, Jelena, Laura Antonucci, and Michael Karin. "Targeting inflammation in...: eaal3118. PubMed PMID: 28495700. 4. Zelenay, Santiago, et al. "Cyclooxygenase-dependent tumor growth...

  12. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    Published
    Nov. 19, 2019, 2:08 p.m.
    ...choose your own protein purification adventure. You want to purify your favorite protein (YFP). You have ...light-oxygen-voltage sensing domain from the oat plant (Avena sativa). In darkness, the AsLOV2 domain folds...15 kDa), single monomeric variable heavy chain antibody domain.  How do these components interact? When...binding ability of its nanobody partner, so it’s important to insert AsLOV2 at a spot in the nanobody that...tagged, which limits these approaches to use with recombinant proteins. Purifying an untagged protein with ...

  13. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...base editors in vivo and achieved therapeutically relevant editing efficiencies in the mouse brain, liver...voltage indicator Article contributed by Shreya Vedantam Genetically encoded voltage indicators (GEVIs)...McCormick lab generated knock-out and knock-in mutants, determined protein expression levels from different...the CyanoGate kit at Addgene. Vasudevan et al., Plant Physiol 2018. PubMed PMID: 30819783. Light up CreLite...PIF6, phytochrome-like fusion proteins from the plant Arabidopsis thaliana. In the presence of red light...

  14. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...not be important for a good friend, but if you are making a professional inquiry, you always want to read...many academic scientists are starting to see the advantages of using social networking to start, build, and...the easiest ways to help is to connect people that want to meet or that you think might be helpful to each...Do not post or comment on anything that is not relevant to business or volunteer organizational work. ...

  15. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...can be both sorted using FACS and isolated with antibiotic selection. The team tested this system in vitro...the addition of standards, the assay is semi-quantitative and can approximate mRNA concentration within...bacteria of clinical significance and four clinically relevant host biomarkers. The lab validated their platform...host-range of the plasmids and suitability of various antibiotic resistance markers, promoters, and inducible ...microbiome responds to infection, dysbiosis, and antibiotic treatment. To facilitate these studies, Pamela...

  16. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    Published
    Feb. 28, 2019, 1:34 p.m.
    ...slides, handouts, and resources were hosted on the Plantae website blog post written by Ben Schwessinger, ...I realized that not all the materials would be relevant to everyone at this specific conference, and I...’s How-to-guide covers everything that you would want to know before, during, and after organizing such...was also recently awarded a Mozilla Science mini-grant, which will fund a project to support a dedicated... and impactful opportunities - There are many brilliant minds out there, but how do you get them to connect...

  17. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...added using PCR-based amplification (Option 2). Advantages of Golden Gate cloning Golden Gate cloning is...potential clones. Golden Gate assembly has a few advantages over other cloning methods. Exonuclease-based...destination vector, making multiplexing easy.  Disadvantages of Golden Gate cloning Golden Gate cloning is...may not be amenable to "domestication", you might want to consider using an alternative method like Gateway...Gateway cloning or Gibson assembly. Another important consideration is the design of flanking overhangs...

  18. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ...other references to scientific literature to substantiate the facts upon which it builds. This means you...your area. When you are in ‘writing-mode’ for a grant or a scientific manuscript, the reading will likely... you an overview of the topic and point you to relevant primary research articles. Then just start reading...key words and download what seems interesting or relevant. Harness the power of the Google search engine...Google Scholar will send you daily updates with relevant literature. Organizing the literature As a scientist...

  19. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the more loosely bound...acetyltransferases or CARM1 methyltransferase. This attendant signaling feature afforded by coactivator complexes...comprehensive datasets that collectively address many important and fundamental questions in nuclear receptor ...states, their circadian regulation, as well as in important physiological states such as adipogenesis and ...coregulators. We look forward to growing this important relationship with Addgene for the benefit of the...

  20. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...altogether different. They are filled with blind anticipation. Their trust in the quality of the content is... manuscript could only have been viewed as a significant improvement. As a result, the software piñata...those engaging in CRISPR design, but it is a significant hurdle made worse by an ever increasing marketplace...for this purpose. This second area is extremely important and given the lack of attention to it, it is an... interact with the software. This category is important for users wishing to select tools based on their...Matchmaker, it provides a list of terms I think are important to define for the CRISPR software space. It is...

  21. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...CRISPR activation uses dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes...themselves. SunTag, SAM, and VPR have all shown significant improvements upon the initial dCas9-VP64 method... this method is its construction: it relies on antibody chains, which are relatively large and are not...Performance Generally, VPR is found to have significantly higher activation levels than the initial dCas9...activation levels are similar to that of SunTag. An advantage to this method compared to other notable CRISPR...

  22. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...Outbred strains of mice are useful for comparing dominant and recessive traits and their impact on diseases... case, a positive selection marker such as an antibiotic resistance gene is also included to select for... are injected into a blastocyst which is then implanted into a female animal for gestation. Nucleases ...are microinjected into a zygote, which is then implanted into a female mouse for gestation. All three can...nucleases to generate an engineered mouse model has advantages over the injection of a targeting vector alone...

  23. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...these communities, which is in turn crucial if we want to make sure that everyone can be informed and empowered...individual consumers of technologies and as societal participants in the political process. My role at pgEd I ...who are interested in such careers should take advantage of their academic environment and attend seminars...either giving or running these events will be important both in preparing you for future work in these...communicate your work to a non-technical audience is an important skill for every scientist. After all, scientific...

  24. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ... would be infected by a lab-grown virus, it's important to understand the risks of the specific virus ...necessary step during production), there is a significant risk for aerosol contamination. As most centrifugation...ethanol (other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol, 0.5% peracetic...sharps procedures for your organization will significantly reduce the risk of an accident (the most common...more likely to make a mistake and more likely to want to cut a few corners. For many researchers, viruses...

  25. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...advocacy non-profits like Phipps Conservatory or the Brilliant Club and share your knowledge at local schools...Student Voice are two examples. Many blogs like Plant Science today, Soapbox Science (hosted by Nature...Science, Media and Communication. In the US, UC Santa Cruz, MIT, UW Madison, and Boston University offer...where you may take a science track. If you don’t want to commit time and money to a full-time graduate...communication. If science journalism is the path you want to follow, check out the distance learning courses...

  26. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...advocacy skills “bootcamp” for a focused group of participants. More information can be found at http://futureofresearch.org...graduate students who work as teaching or research assistants to join unions. The topic of unionization continues...productive routes for advocacy efforts and help participants develop the efficacy of their advocacy skills...of Howell Communications Philip Brenner, PhD - Assistant Professor of Sociology. University of Massachusetts... Developing effective science advocates is an important way to push the balance from challenge to opportunity...

  27. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...strength as a determinant of gene expression levels, the terminator also plays an important role in RNA ...expression vectors use double terminators to reduce unwanted translation of downstream elements. A high affinity...polyadenylation. The addition of the poly(A) tail is important for stability of the mRNA, protection from degradation...Maitland NJ. An Internal Polyadenylation Signal Substantially Increases Expression Levels of Lentivirus-Delivered...

  28. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...at my workshops, I get nods from nearly every participant. Robert Bramson, author “Coping with Difficult...people: Hostile-aggressives, who are usually antagonistic and impolite, Complainers, who spend most ...people. Some students and postdoctoral fellows are hesitant about expressing their ideas, especially if their...solutions that will meet both of your needs. 6) Put important agreements in writing One of the most frequent...from your supervisor. Whenever possible, discuss important issues in person or over the telephone instead...

  29. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ...regulation, but enhancers and chromatin looping play important supporting roles. Enhancers are cis regulatory...ligation creates chimeric circles of DNA that are distant linearly but close together in space. The chromatin...interactions. The chromatin story told by CAPTURE 2.0 is important, but it’s not the only plot line in gene regulation...CRISPR users Learn how to Choose the Best Cas9 Variant for Your Next Experiment Learn More About Designing...Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic Regions of Interest Here...

  30. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ...design, batch primer design or batch analysis. We wanted to relieve scientists of the burden of having to... to analyze  CRISPR repair outcomes, which is important for quality control. The only ones we found that...that worked with NGS data were: Crispresso CrispRVariants AmpliCan We chose to adapt Crispresso by Luca...their "summarisation score".) For CrispyCrunch, we wanted to consider both factors, as a bench scientist ...coding region in a UTR, CrispyCrunch flips the two important base pairs of the PAM (NGG -> NCC, CCN -> GGN)...
Showing: 511 - 540 of 885 results