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  1. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...optimized for your application. Depending on the tag, directly imaging or tracking your protein may also be possible...is not ideal with Myc, as low pH conditions are required during the purification, which may affect protein...

  3. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...: Do not cut within your insert Are in the desired location in your recipient plasmid (usually in ...amplified (the ORF in this case), not the Tm of the entire primer. If you are amplifying from a plasmid or...

  8. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...ml This method is quick and simple and doesn’t require any special reagents. However, there’s limited ...absorbance-based methods, fluorescence-based methods require a standard curve, a set of samples with a known...

  10. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...Berkeley’s Jennifer Doudna. “They are the first direct demonstration of use of genome editing to correct...need to get a handle on how cleavage sites are repaired.” Those insights will be critical for scientists...

  12. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ... presence influences cell fates. The atlas of directed differentiation To understand how each human ...Joung J. et al., A transcription factor atlas of directed differentiation. Cell. 2023. 186 (1) 209-229. ...

  13. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...illuminated with green light. They found that KillerRed required longer exposure times (5 minutes vs 10 seconds...longer exposure times. Additionally, KillerRed requires mitochondrial targeting to increase cell ablation...

  21. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...response, the variable region of an antibody is paired with any of the other isotype heavy chain constant...deleted.     Figure 2: Isotype switching requires DNA recombination of the antibody gene locus. ...

  23. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...clarified, especially with regard to licensing models. Direct sharing of work from universities and research ...Deepak Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew Chai, Manu Prakash, "Squid: Simplifying ...

  24. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...new ligands for unknown GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth...chemogenetics receptors, GPCRs were first mutated via site-directed mutagenesis to bind non natural ligands. These...

  27. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...targeting efficiency of only 27.9% and homology-directed repair (HDR) taking place in 41.2% of edited cells...NHEJ rather than HDR. Figure 1: Homology-directed repair in targeted zygotes proceeds from the maternal...

  29. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...of ccdB, leading to positive identification of desired clones.   ...Becomes a powerful cloning tool... efficiently, as those that do not contain the desired insert should not grow.   Figure 2: Image...

  30. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...those planning a career in academia, it may not be required, but many academic scientists are starting to ...use LinkedIn messaging, not everyone likes to. A direct email can be the best way to get in touch if you...
Showing: 511 - 540 of 758 results