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  1. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...and Geneious, I was able to determine if any discrepancies between what we sequenced in house and the submitted...

  3. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...preparation in E. coli. Addgene depositor Indu Maiti has created a new and versatile binary Ti vector for both transient...

  10. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ... foreign DNA into another cell. Minimally, lab-created plasmids have an origin of replication, selection...

  11. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...resin. The MBP tag, although large, can actually increase expression and solubility of the tagged protein...

  12. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...immune response against a pathogen. The Fc domain creates a link between the antibody and other parts of ...

  13. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...mutating these sequences. Pro tip! Various labs have created Cas9 enzymes with looser PAM requirements, which...

  17. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ... of combining “new discoveries or technologies increase chances that your project will be fresh as well...

  20. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ... lunch or beer) and in 1:1 conversations. This creates a level of familiarity and confidentiality to ensure...

  26. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...to bind to a previously hidden epitope (right). Created withBioRender.com.   Biological Processes  ...

  28. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...FLAG, but can be more!) to a protein’s termini to increase detection. The tag can also be removed by treating...

  29. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...for the transduction step. Viral particles are secreted into the cellular media, making viral collection...

  34. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...only need to skim the alphabet on the side of the screen to find the sequence for your enzyme of interest...

  35. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...points. At Addgene, we like to do this live on the screen using a Google Doc. Action items: Before leaving...

  38. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...these projects could also lead to novel yeast creations that may not be pursued in an academic or industry...

  39. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ... skills, which are useful to many employers. Increasingly, computer skills are highly desired, as roles...
Showing: 681 - 720 of 788 results