We narrowed to 920 results for: Gene
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TypeBlog PostPublishedJan. 18, 2024, 2:15 p.m....disponible en español! Esta traducción fue regalada a Addgene por Daniel Mejías Sánchez, ¡y no podríamos estar...emocionados! Después de recibir la traducción, Addgene contrató a una traductora científica profesional...Descargar CRISPR 101: Una herramienta de edición Addgene tiene una gran audiencia internacional, incluido...available in Spanish! This translation was gifted to Addgene by Daniel Mejías Sánchez, and we could not be more...more excited! After receiving the translation, Addgene contracted with a professional scientific translator...Download CRISPR 101: Una herramienta de edición Addgene has a large international audience, including working...
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Enabling high school research at the Journal of Emerging Investigators
TypeBlog PostPublishedSept. 5, 2018, 12:01 p.m....Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast. Additional... In this episode of the Addgene Podcast, we introduce you to the Journal of Emerging Investigators, an...recently submitted to the journal with funding from Addgene. Listen to the interview with the Journal of Emerging...Additional Resources from the Addgene Blog Find education resources Use our Plasmids 101 eBook to teach ...genome enegineering technology Resources at Addgene.org Find basic molecular biology protocols Check... -
Selecting Your Plasmid Purification Kit
TypeBlog PostPublishedMay 27, 2025, 1:15 p.m....kit to use? Obtaining higher quantities of DNA generally means starting with higher volumes of bacterial...reduce your lab’s environmental impact, check out Addgene’s blog post on bringing sustainable practices for...Monarch®, QIAGEN’s QIAwave, and Milllipore Sigma’s GenElute™ kits were all specifically designed with environmental...Series NEB Monarch QIAGEN QIAwave Millipore Sigma GenElute Reduced waste from kit materials Reduced plastic...relatively little work after the initial setup. (Addgene actually uses some of these — if you’re curious... (These are mostly miniprep kits! Larger kits generally take longer.) Make sure to take a look at the...Nyla Naim. Resources Additional resources on the Addgene blog Plasmid Preps: Different Purity, Different... -
Twelve Amazing Guest Blog Contributors!
TypeBlog PostPublishedNov. 26, 2024, 2:15 p.m....intern who shared their experience working at Addgene. An Addgene Summer Internship Matteo Tardelli, who ...contributing to the Addgene blog? We'd love to hear from you! Email us anytime at news@addgene.org. ... very thankful time of year for those of us at Addgene! And we can’t think of any better way to start ...to the blog. Thank you! More resources on the Addgene blog 10 Great Guest Posts We're Thankful For Lucky... -
DIY DNA Ladders from Penn State University
TypeBlog PostPublishedJuly 14, 2017, 2:30 p.m....molecular weight ladders were recently deposited with Addgene. A team of undergraduate students led by Dr. Song...restriction digested with PstI or EcoRV, these plasmids generate 100 bp or 1 kb DNA ladders, respectively. Unlike...pPSU1 and the pPSU2 plasmids are available from Addgene. Learn How to Analyze a Restriction Digest References.... L., & Tan, S. (2017). The pPSU Plasmids for Generating DNA Molecular Weight Markers. Scientific Reports...Central PMCID: 5446395. Additional Resources on the Addgene Blog Are you a Frugal Scientist? Save Money with...the Evolution of Lab Techniques Resources on Addgene.org Use the Kit-Free Alkaline Lysis Plasmid Miniprep... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m....production requires animal sacrifice and precludes genetic engineering of a tag or fusion with a reporter ...described in Pleiner et al, the key steps for generating nanobodies are outlined below; no alpaca required...your work. Species Antibody Subclass Nanobody Addgene Plasmid Rabbit N/A* TP897 pTP1183 (1x cysteine...nanobodies from the column. bdNEDP1 protease can be generated using plasmid pDG02583. Label the Nanobodies: ...29263082 Find plasmids from this publication at Addgene. 2. Bates, M., Böhning, M.I., Chug, H., Görlich...PubMed PMID: 23495938. Additional Resources on the Addgene Blog DIY DNA ladders from Penn State University... protein expression Additional Resources on Addgene.org Find more nanobody expression plasmids here ... -
5 Science Rap Videos & Their Creator: Tom McFadden
TypeBlog PostPublishedFeb. 9, 2017, 3:30 p.m....Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast. Additional...Plasmid Rap Plasmid rap video made with support from Addgene. Check out more science education tools...looking for creative ways to implement the next generation science standards and communicate science. Learn...Additional Resources on the Addgene Blog Get Advice on How to Build a Grad Student Community Check out All...Plasmids 101 Series Additional Resources on Addgene.org Check out Our Protocol Pages for New Protocol... -
Call for Guest Bloggers
TypeBlog PostPublishedMarch 2, 2016, 3 p.m.... Sign up to write for the Addgene blog! To sign up to write for the Addgene blog simply email us. We can...write for us! Click Here to Contribute to the Addgene Blog Possible guest topics Some topics you might...Exposure - With over 100,000 views each month, the Addgene Blog is a great way to get your name out there!...include on CVs and Resumes. An Expanded Network - Addgene will promote your blog on a variety of social media...applying CRISPR for mouse genome editing. Post: Generating Mouse Models Using CRISPR/Cas9 Erik L. Snapp... -
#DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La
TypeBlog PostPublishedDec. 2, 2021, 2:15 p.m....our best guess). Follow @Addgene, use the hashtag #DeckTheLab, and tag @Addgene in the post! In January...It’s finally December, and here at Addgene HQ in Massachusetts that means it’s time for near-freezing...winner, you’ll receive a winter care package from Addgene. Here are the details: Contest 1: Deck the ...CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com...however you celebrate! And if you want to see how Addgene celebrates and decorates, follow us on Twitter ... -
Newly Updated AAV Data Hub!
TypeBlog PostPublishedFeb. 7, 2023, 2:15 p.m....experiments, let us know (datahub@addgene.org) and we might just send you a new Addgene Data Hub t-shirt! ... out the redesigned, revamped, and re-launched Addgene AAV Data Hub, the place for scientists to share...curated collection of community submissions for Addgene and CLOVER AAV experiments - and we've updated ...Submitting to the Data Hub If you've ever validated an Addgene or CLOVER AAV, you can (and dare we say, should...step-by-step submission guide or email datahub@addgene.org to learn more. A great submission includes: ... -
2022 and 2023: Reflections and Looking Forward
TypeBlog PostPublishedFeb. 23, 2023, 2:15 p.m....quietly learning about Addgene’s past and present, but the real promise of Addgene lies in our future. As...Addgene saw big changes in 2022, from the launch of a new material to the redesign of a community resource...collection, which includes both neuroscience and general-use antibodies, is available in both trial- and...organization. One thing that will remain the same is Addgene’s commitment to supporting the research community...number of people. I look forward to working with Addgene colleagues, collaborators, partners, and the larger... -
Science Communication Snapshot: DayCon 2015
TypeBlog PostPublishedJune 17, 2015, 9:04 p.m....accessible to the general public both in terms of cost and content level. Sponsorship from Addgene and Harvard... Here at Addgene, we’re dedicated to advancing and sharing science! In association with the Harvard graduate...DayCon. DayCon is a one-day conference aimed at the general public that provides an accessible introduction...introductory lectures targeting three broad concepts: the genetic basis of disease, cancer, and viral infections.... do you share your science with the community? Addgene would love to hear about your efforts to engage... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog PostPublishedJuly 10, 2018, 1:08 p.m.... Biology Resources on the Addgene Blog Deposit your plasmids with Addgene and make your work more accessible...copes with the growing amounts of digital data generated in labs. While many labs approach the situation...are available on the market today, ranging from general note taking tools, to more scientific field-based...One comment reflected the desire for a flexible generic solution, rather than an ELN designed for a specific...From pictures to excel sheets, researchers today generate vast amounts of digital data during their work...Twitter @scinoteELN Additional Resources on the Addgene Blog Learn about more tools for reproducibility... -
Filming Science Videos in the Age of Social Distancing
TypeBlog PostPublishedMay 20, 2021, 1:15 p.m....Are the shots clear enough for the audience? As Addgene’s Science Video Production Specialist, I’m constantly...March 2020, just four months after I started with Addgene, I was about to have a check-in with my boss Jennifer...Cycle 3: A Return to HQ Figure 3: Even Blugene is practicing social distancing and wearing their...traditional solution was completely out of reach for Addgene. So, onto research! I needed to design a system... needed to figure out a microphone situation. Generally, I record into an external recorder using a lav...the Lab Design: The lab is the beating heart of Addgene. It’s why we exist, storing plasmids, and distributing... out all of the written and video protocols at Addgene? If you need help in the lab, we have you covered... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog PostPublishedNov. 25, 2015, 4 p.m....Addgene recently attended and sponsored the Future of Research (FOR) Boston Symposium 2015. I personally...fun and surprisingly exhausting experience. As Addgene believes many of the topics discussed during the...are many companies and organizations (including Addgene), that need people who are data oriented, scientifically...skills beyond those required at the lab bench. At Addgene we try to do our part in boosting awareness of ...write for a university publication, improving your general writing skills will help you in your academic publishing...problems within the research environment itself. At Addgene we hope that all parties involved in making academic...Publications of Interest Can Be Found Here Resources at Addgene Read David Riglar's FOR Boston 2015 Blog Post... -
What's New in CRISPR - December 2019
TypeBlog PostPublishedDec. 17, 2019, 2:37 p.m.... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR... optimized for Burkholderia, they identified 67 genera of Proteobacteria that this toolkit might be used...TadA) to a catalytically impaired Streptococcus pyogenes Cas9. They called this editor Target-ACE (adenine...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by... -
Scientific Sharing in the Time of COVID-19: Databases and Resources
TypeBlog PostPublishedMay 12, 2020, 1:15 p.m....thread ⬇️⬇️⬇️ — Addgene (@Addgene) April 15, 2020 Additional resources on the Addgene blog Read about...essential research into COVID-19 (like here at Addgene!), and new databases are supporting their access...” said Guillaume Bourque, a Professor in Human Genetics at McGill University. “There is a need to pool...guide for external collaborations Resources on Addgene.org Browse our COVID-19 plasmid collection ... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog PostPublishedNov. 6, 2015, 3:30 p.m....people and society. Future generations of IMP Motivating future generations is key to the success of this...from international financial struggle, our new generations feel thrown, to sink or swim on their own, into...knowledge, experience, and new professional capacity generated during this process result directly in positive... growing demand from universities and future generations of professionals has made possible a significant...School, where she studies age-related macular degeneration. She is also a blogger who writes posts for ...Advancemnet of Higher Education website Resources at Addgene Read Other Career Blog Posts Read the Networking... -
We're Updating Our Hold for Publication Policy
TypeBlog PostPublishedJune 24, 2025, 1:15 p.m....sometimes unclear or missed altogether. Emailing Addgene might not be as high on the published-paper-celebration...in this status — a win-win situation for all. Addgene will release plasmids on hold if they are clearly...celebrate!), send us a quick email at deposit@addgene.org with the citation and your deposit or plasmid...distribute unpublished materials. What about preprints? Addgene supports preprints and encourages authors to share... for reading, and for depositing plasmids with Addgene! We'll continue to review our policies to make ...website, Help Center, or email us at deposit@addgene.org. ... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog PostPublishedSept. 14, 2017, 12:40 p.m....Additional Resources on Addgene.org Brush Up on Your CRISPR Background with Addgene’s CRISPR Guide Page Find... heat loving) bacteria, such as Streptococcus pyogene’s SpCas9. These Cas9 proteins don’t work well at...try, as always, the plasmids are available from Addgene. Happy CRISPRing! References Harrington, Lucas...bioRxiv (2017): 138867. Additional Resources on the Addgene Blog Read about CRISPR Methods for Bacterial Genome... Find CRISPR plasmids at Addgene ... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m....language is ... The general representation of data is... The figures are ... In general, I think that this...language in the first (and clunky) version of my general comments and adjust them if my opinion changed ...can be deposited in the specific repositories (Addgene, WormBase, Arabidopsis.info). These repositories...PMCID: PMC4528400. Additional resources on the Addgene blog Find blog posts on scientific sharing Get...tips on writing a review article Resources on Addgene.org Learn about depositing plasmids pre-publication... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog PostPublishedJan. 25, 2018, 2:09 p.m....involves amine-reactive biotin derivatives that generate covalent bonds with positively-charged amino acids...solution was to exploit the best of both worlds. We generated a plasmid that produces biotin ligase and a protein...the biotin ligase BirA backbone (Eric Campeau, Addgene plasmid #26624) was unsuccessful. Nothing more ...pMal-T-Avi-His/BirA plasmid now available through Addgene. We have successfully used this plasmid to biotinylate...cell signaling. Additional Resources on the Addgene Blog Check out other Hot Plasmids Find Additional...Posts on Plasmid Technologies Resources at Addgene.org Learn about Popular Plasmids in the Repository... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog PostPublishedJune 22, 2021, 1:15 p.m....about pseudotyping Resources on Addgene.org Find plasmids in Addgene's COVID-19 collection Find SARS-COV...coronavirus and SARS-CoV. These two viruses are quite genetically distinct from SARS-CoV-2 and its variants. Yet...Balazs lab was quick to deposit their plasmids at Addgene to accelerate ongoing COVID-19 research. “I hope...j.cell.2021.03.013 Additional resources on the Addgene blog Browse all COVID-19 related blog posts Learn...-COV-2 pseudotype virus plasmids from Addgene ... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog PostPublishedApril 28, 2021, 1:15 p.m....writing for a general audience. The tool highlights jargon terms or words rarely used in general writing in... defining terms. The phrases in Phrasebank are generic, so they need to be edited to suit your particular...desired formatting style. This tool is great for generating citations for a few papers at a time. Mendeley...method in grad school Additional resources on the Addgene blog: Tips for writing scientific manuscripts:...writing a scientific review article Write for the Addgene Blog ... -
Antibodies 101: Flow Cytometry Controls
TypeBlog PostPublishedJune 6, 2024, 1:50 p.m.... Cytometry Resources on Addgene.org Addgene's Antibody Guide Browse Addgene's Antibody Collection ...single color controls On a flow cytometer, you generally want your positive cells to read between 104 and...isotype controls, while others don’t. However, the general consensus seems to be that if you are looking for...Harvard Medical School. More resources on the Addgene blog Introduction to Gating in Flow Cytometry Antibodies... -
Clear and Accessible Writing
TypeBlog PostPublishedFeb. 15, 2024, 2:15 p.m....Addgene was built on the concept of accessibility: making plasmids more accessible to scientists around...the next twenty years. But learn we did! From Addgene's very beginning, we had to consider digital accessibility... accessibility on our website. As members of Addgene’s content team, we — Jo, Brook, and Angela — most... in a poster compared to an online abstract or Addgene blog post. Here, we are presenting this image ... with the rest of the workshop. For Figure 1, Addgene chose this alt text: "The left panel is a profile...writing! Resources Additional resources on the Addgene blog Five Steps to Writing Grant Proposals to Grad... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog PostPublishedJune 2, 2016, 2:30 p.m....communication and epigenetics. Follow her on twitter @kc_palozola. Additional Resources on the Addgene Blog ...keep track of the files that I was continuously generating. I quickly found that there is no one way to...physical lab notebook, along with any figures generated from the analyses: Goal: Before you begin, briefly...control your commonly used scripts: If you edit a general purpose script for a single use, save the script...Software Matchmaker Additional Resources on Addgene.org Find Protocols for Your Next Experiment Find... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog PostPublishedFeb. 28, 2019, 1:34 p.m....workshops and organizational skills. Meanwhile, Addgene was also thinking about ways to address irreproducibility... to Teytelman’s connection with Joanne Kamens, Addgene also had the opportunity to contribute to this ...being adopted by a wider group of researchers. Generating material to empower others to practice and teach...anyone could present the material. For example, Addgene’s contributions by Tyler Ford included reagent sharing...Tsang for editing. Additional resources on the Addgene blog: Check out this guide on Transferable Skills...workshops Levelling Up: Reproducibility for Everyone Addgene: a global resource working to enhance scientific... -
Antibodies 101: Fab Fragments
TypeBlog PostPublishedSept. 5, 2024, 1:15 p.m....Mess in IHC Resources on Addgene.org Browse ready-to-use antibodies at Addgene...visualize a Y-shaped protein reminiscent of the Addgene mascot Abi? If so, you are not alone. Full-sized... divide up the different regions, both through genetic and proteolytic approaches. This comes in handy...when that might be later). Find scFv plasmids at Addgene Depending on how an antibody is broken up, there...j.crmeth.2022.100301 Additional resources on the Addgene blog: Introduction to AntibodiesSingle Chain Fragment... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....interaction. In as little was one minute BASU generated a strong streptavidin signal (~250-fold enrichment...point, but one minute of labeling with BASU still generated a >30-fold enrichment compared to E. coli’s BirA...host protein critical to Zika replication. Human genetic disease. RaPID can also be used to test how RNA... answer? The RaPID plasmids are available from Addgene! References Ramanathan, M., Majzoub, K., Rao, ...PMCID: PMC4054858. Additional Resources on the Addgene Blog Study Protein-DNA Interactions with CUT&RUN...resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids Find CRISPR plasmids... -
Antibodies 101: Secondary Antibodies
TypeBlog PostPublishedDec. 21, 2021, 2:15 p.m.... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...antibodies work? Secondary antibodies are antibodies generated against antibody isotypes from a specific species...classes, it is possible to get secondary antibodies generated against isotype subclasses, such as mouse IgG2...my goat anti-rabbit?!”* *a secondary antibody generated in goat against rabbit immunoglobulins. References...References and Resources More resources on Addgene’s blog Antibodies 101: Introduction to Immunofluorescence... -
Antibodies 101: Conjugation
TypeBlog PostPublishedMarch 26, 2024, 1:15 p.m.... Resources on Addgene.org Unconjugated antibodies at AddgeneAntibody plasmids at Addgene... functionality of the conjugate. For example, Addgene’s antibodies are provided in a buffer with the antimicrobial... Schibli R (2015) Antibody Conjugates: From Heterogeneous Populations to Defined Reagents. Antibodies ...10.1038/nmeth.4380 Additional resources on the Addgene blog Antibodies 101: Buffers, Storage, and ConjugatesAntibodies... -
Developing Lab Management Software for Biology
TypeBlog PostPublishedAug. 25, 2016, 2:30 p.m....placed on the Addgene website, it sets off a chain reaction of events throughout all of Addgene - within the... of thousands of plasmids a day, like we do at Addgene, pen and paper will fail you. The benefits of lab...fun too. Kris Shamloo is a Software Engineer at Addgene and is interested in writing software tools to ...krisshamloo.com Additional Resources on the Addgene Blog Learn about How We Use Barcodes in the Lab...Track Your Experiments Additional Resources on Addgene.org Find Plasmids for Your Research ... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog PostPublishedOct. 22, 2019, 12:20 p.m....interesting discoveries. You’ve analyzed the data and generated beautiful figures. And, you can’t wait to tell...need to first make your poster. While a poster generally contains the same sections as a primary research...references and contact information, and it is generally acceptable for the font here to be much smaller...it’s good to let your ingenuity shine, and you generally have free reign over how you put your poster together... as rows. It can be more catchy because it is generally less common and will stand out; however, be careful...make it your own! Additional resources on the Addgene blog Check out this blog post on navigating the... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog PostPublishedAug. 9, 2022, 1:15 p.m....monoclonal antibodies; Addgene collaborates with these companies to create and provide general availability of...recombinant forms (rAbs). mAbs and rAbs have higher homogeneity than polyclonal antibodies because of their immunoglobin...that can be produced in large “copies.” This homogeneity circumvents the batch-to-batch variability found...that the primary antibody is very weak or with general non-specific binding. If the sample differs from...10.1371/journal.pbio.1002165 More resources on the Addgene blog Antibodies 101: ValidationSelecting the Right... -
Tips to Make the Most of a Scientific Conference
TypeBlog PostPublishedMay 23, 2019, 12:26 p.m....skill set Resources on the Addgene website Become of a member of the Addgene depositor community ...a conference, be sure to practice beforehand. Generally, two types of people will visit your poster: those...the tweep up (and for the entire conference, in general). After the conference Review your favorite conference... considered networking, but here are some more general tips. Before the conference Networking is key even...your scientific conference? Resources on the Addgene blog Read other posts on scientific sharing, scientific... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....GFP/EGFP) for a transcriptional reporter or as a general cytoplasmic label of cells, there's no problem.... of the FP. The FP is confined to a plane and generally only rotates in 360°, increasing the probability...can be monomerized with an A206K mutation [2]. Addgene has many of these constructs and they are designated...PMCID: PMC3324619. Additional Resources on the Addgene Blog Read Professor Snapp's Post on mOX FPs Find...in Your Experiments Additional Resources on Addgene.org Browse our Fluorescent Protein Resources Find...Plasmids that Glow (Luciferase Plasmids) Find Optogenetics Resources ... -
Antibodies 101 Series on TikTok
TypeBlog PostPublishedMarch 19, 2024, 1:15 p.m....animation is becoming a TikTok series! Follow @addgene on TikTok for bite-sized portions of antibody concepts...an antibody?" or "what is an IgE isotype?" @addgene Ready to learn about antibodies? Our new antibodies...molecularbiology #science #scicomm ♬ original sound - Addgene We'll be posting these animations regularly ... all of our videos will also be shared on our @addgene Instagram account, or you can watch the full animation...on our YouTube channel. More resources on the Addgene blog Antibodies 101: Introduction to Antibodies... -
Trends in CRISPR and SynBio Technologies
TypeBlog PostPublishedFeb. 4, 2015, 3:58 p.m....program are here. This was a great opportunity for Addgene to present our own data on plasmid deposits and...and distirbution for these fast moving fields. Addgene is a global nonprofit plasmid repository. Over ...Over 2,000 labs have deposited plasmids to Addgene and we distribute over 130,000 plasmids in 2014. Thus,...combination of open-source distributors such as Addgene, as well as a number of online user forums such...Meeting Eric presented a poster with data comparing Addgene global deposit and distribution trends for Zinc... -
Antibodies 101: Immunoprecipitation
TypeBlog PostPublishedDec. 7, 2021, 2:15 p.m....resources on the Addgene blog: Introduction to Immunofluorescence View all of Addgene's antibody posts ...Resources on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene ...that interact with nucleic acids. Labs studying epigenetics routinely use the chromatin IP (ChIP) technique... found this blog helpful be sure to check out Addgene’s blog for other antibody topics. References...