...part of my job, in addition to preparing DNA and generating/replenishing kits, is to find new ways to make...extract libraries from previously fragmented DNA.The general process for library preparation using this kit ...total of about 3 hours. It was robust, capable of generating a wide range of insert sizes and also produced...participants from different biotech companies to interact and learn more about one another. They also provided...representatives. I got to talk with a technician from Neon Therapeutics, a biotechnology company that uses neoantigen...extremely helpful since she was familiar with library generation. Also, I now know what to expect at a workshop...

...2012). The polarizing impact of science literacy and numeracy on perceived climate change risks. Nature... between what I was doing in the lab and the interactions I was having outside of the “ivory tower.” I... acute “adeolitis” – received the best ratings overall, simply because none of the participants had preconceived...show that people with greater levels of science literacy are especially prone to this, possibly because...Fischhoff, B. (2017). Individuals with greater science literacy and education have more polarized beliefs on controversial...

...for a higher number of MPE. Therapeutic potential  To explore the therapeutic potential of the DAP array...pathogen. Over the past decade, CRISPR tools have leveraged such host-defense mechanisms to enable multiplex...to develop a strategy that would independently generate multiple gRNAs for nCas9-BE, or any other kinds...drive-and-process.” To optimize DAP use, they developed a generalizable method to acquire the most effective DAP array... demonstrated up to 31-loci MBE with over 50% averaging editing efficiency in human cells. MBE with DAP...

... systems that measure biological phenomena by generating an output which is directly proportionate to ...antibody, enzyme, DNA, etc. The receptors job is to generate a quantitative signal upon recognizing the analyte... (fluorescence, pH, etc.). The transducer will generate a numerical value which represents the amount ...disciplines! Most of the sensors designed for calcium generate a fluorescent output, which will turn on, change... It’s a good thing, though! Viral delivery has several advantages over other mechanisms. For one, many...permanently integrate and/or express long term (up to several years!). Biosensors tend to be more reproducible...

...more effectively used in a technology or as a therapeutic.  A (very) brief history GCE came onto the synthetic...specific to the ncAA and orthogonal to (i.e. non-interacting with) the cells’ own natural translation machinery...system (including media, cell type, and growth temperature) may need to be individually optimized for each...focused on turning GCE tools developed in the literature into robust and universal techniques that researchers...fulfill their mission. While they’ve shortlisted several protocols, including their phosphoserine ncAA protocol...., Griffith, M. C., & Schultz, P. G. (1989). A General Method for Site-specific Incorporation of Unnatural...

... these interactions, and can give valuable insight into DNA expression and protein interactions, including...technique that provides insight into protein:DNA interactions. ChIP works by using antibodies to capture protein... focuses on the DNA your protein is actively interacting with, reducing background information. Your options...could mean needing to increase crosslinking to counteract the effects of increased sample agitation; a ... this investment can well be worth it once you generate your data. However, it may be a good idea to add...

... In each case, we leveraged the team’s knowledge of the scientific domain in general and of our data in... that we needed to improve more than that: the general “findability” of the information on the site. Different...approached the issue from multiple angles: the overall user experience of our website, the different website...resources and handle millions of queries. In an iterative process driven by our user experience experts,...approach much more than if we were dealing with general purpose information. Below are a few of the strategies...

...proteomics--the study of the distribution and interactions of proteins within these structures. Enzyme-...substrate to map the spatiotemporal distribution and interaction networks of specific proteins in living cells...process is much slower and can take as long as several days. In 2015, the laboratory of long-time Addgene..., the enzymes function across a much broader temperature range than BioID, which only functions optimally...modular domains that facilitate protein-protein interactions and mediate biochemical processes. One of the...than previously thought. Sidhu’s group has made several hundred SH3 domain containing plasmids available...

...synthetic) pFZ19 (yellow flame) Temperature inducible promoters Temperature sensitive expression systems ...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...Types of inducible promoters Chemical agents, temperature, and light are all examples of factors that can...promoters; they show near-zero expression at regular temperatures but can be induced by heat or cold exposure....since expression can be repressed by glucose. Temperature inducible promoters are also known for their ...

...interest, as it is prone to generating indel errors. Indel errors generated in the course of repair by ...vast toolbox of processing factors, including polymerases (Pol μ and Pol λ), nucleases (Artemis), and structure-specific...illustrated below, NHEJ-mediated repair of Cas9-generated breaks is useful if the intent is to make a knockout...overhangs is available. Cas9 can also be altered to generate a targeted single strand break; when two such ...Chromosomal Translocations in Human Cells Are Generated by Canonical Nonhomologous End-Joining. Molecular...

...a high imidazole buffer that outcompetes the interaction between the protein of interest and the metal...polyhistidine tagged protein of interest (purple) and several contaminating proteins (blue, green, orange) flows... low imidazole wash buffer disrupts the weak interaction of the contaminating protein but not that of ...A high imidazole elution buffer disrupts the interaction of the protein of interest and it is successfully...protein conformation, the polyhistidine-metal ion interaction is not disrupted by denaturants (Hochuli, 1988... of a protein. Neither the N or C terminus is preferable over the other however, tag placement can affect...

...PubMed PMID:29483642. Making protein-protein interaction studies easier with a 2in1 vector cloning system...cloning system is well suited for protein-protein interaction (PPI) studies in plants that rely on transient...Grefen Lab has utilized the 2in1 cloning system to generate a series of plasmids to study PPI using Förster...Plasmid Toolkit consists of 4 plasmid toolkits generated with 4 different, binary 2in1 vectors with all...powerful approach to control protein-protein interactions in cells because the protein binders can be ... that these photo-reversible protein-protein interactions can control subcellular localization processes...

... moment that Teytelman realized that running interactive workshops to train researchers on tools and best...next steps. April Clyburne-Sherin had already run several reproducibility workshops as Director of Scientific...to this open science collaboration. The first iteration of these workshops took place at Caltech in Jan...being adopted by a wider group of researchers. Generating material to empower others to practice and teach...team was assembled when it was realized that interactive workshops that train and provide researchers ...on the Addgene blog: Check out this guide on Transferable Skills: External Collaborations Learn about ...

...way they are, the research antibodies in your refrigerator often need a little help to be useful. Mainly...enzymes. The most common enzyme conjugates are Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP...This chemical coupling can be achieved through several different mechanisms. Most take advantage of chemical...sequence or that rely on strong protein-protein interactions with other peptides, like an Fc-binding domain...acids or peptide tags. 5) Strong protein-protein interactions with other peptides. Created with BioRender....Ligation Assay for Detecting Protein-Protein Interactions and Protein Modifications in Cells and Tissues...

... only for translational research, such as gene therapy, but also basic research. To improve cell-type ... with high rates of labeling are ideal of gene therapy applications, promoters that produce sparse labeling...makes it easier to trace a neuron’s projections. Generating animal models: Transgenic animals allow for the...tissues, but they are costly and time-consuming to generate, especially for large animals such as primates...basic as well as translational research for gene therapy. The Roska lab hopes to design improved versions...blog Read more about using AAV for retinal gene therapy Find more about precise neuronal targeting using...

...interesting discoveries. You’ve analyzed the data and generated beautiful figures. And, you can’t wait to tell...need to first make your poster. While a poster generally contains the same sections as a primary research...references and contact information, and it is generally acceptable for the font here to be much smaller...is better! Your poster should be readable from several feet away. It can also be useful to use two different...it’s good to let your ingenuity shine, and you generally have free reign over how you put your poster together... as rows. It can be more catchy because it is generally less common and will stand out; however, be careful...

...prep is straightforward and rapid as there are several commercially available p24 ELISA kits that can ...plasmid was used in the transfection; if preparing several different lentiviruses in parallel, this approach...expansion, and confirmation. This procedure can take several months but should be considered in labs that will...virus or require precise titers. Additional considerations for titering lentivirus Volume and transduction...4.4 (2009): 495-505. PubMed PMID: 19300443. 2. Geraerts, Martine, et al. "Comparison of lentiviral vector...predicting gene transfer events." Genetic vaccines and therapy 2.1 (2004): 1. PubMed PMID: 15291957. PubMed Central...

...and their use for HDR editing and mouse-model generation. Key components of Nanoblades Nanoblades are ...hematopoietic stem cells (HSCs) using Nanoblades. Overall, editing efficiencies were between ~50-70% for ...lower rates of off-target effects. Nanoblades generated up to 76% editing efficiency in mouse BM cells...-target effects, with electroporation of RNPs generally having lower off-target edits compared to DNA ...gRNA, Cas9, and a HDR donor template DNA can be generated by incubating Nanoblade particles with polybrene...Nanoblades are a potential alternative method for generating transgenic mice strains and for non-invasive ...

...them to see how feasible they were, conducting literature reviews and having weekly journal clubs outside...this process, we had an idea that survived the literature review, survived technical feasibility analysis...evenings on the project, but still continued to generate new ideas as they popped into our heads. Anything...color change until a few months later. RevBio accelerates with help from SynBio Axlr8r We limped along ...business. We ended up applying to a business accelerator freely stating that we had an idea, knew how ...rough day, and we have great partners in this accelerator program who want us to succeed. We’re excited...

...DNA, the CTD is also sometimes called the PAM-interacting (PI) domain. The guide RNA is bound primarily...most likely through unstructured electrostatic interactions. It also can’t recognize PAMs, so it associates... less stringent recognition mechanism that can tolerate mismatches in this region. The crystal structure.... As mentioned above, however, Cas9 sometimes tolerates mismatches in the next 8-10 bases after perfect...this region of Cas9 also allows the protein to tolerate RNA:DNA mismatches between base pairs 18 and 20...Each new structural insight also spurs the next generation of engineered CRISPR enzymes with their own tradeoffs...Molecular graphics and analyses performed with UCSF ChimeraX, developed by the Resource for Biocomputing, Visualization...