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We narrowed to 830 results for: Eras

Showing: 341 - 360 of 830 results

  1. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    Published
    Nov. 12, 2019, 2 p.m.
    ...previously yielded valuable enzymes. Famously, Taq polymerase – from Thermus aquaticus bacterium – was discovered...processing environmental samples Since the high temperatures and unstable ground around these hot springs...collection site, we took GPS coordinates, water temperature readings using a heat gun, and pH measurements... continued profiling samples, and years later, several whole genome sequencing projects were launched ...Parvibaculum lavamentivorans, whose optimal growth temperature is 30°C. Find IgnaviCas9 here! What can you do...applications where reagents must be stored at elevated temperatures for long time periods and workflows where CRISPR...

  2. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...Thailand, our annual operating budget is approximately 20,000 USD and we are working on several genetic diseases...: This year, the BeHEARD (Helping Empower and Accelerate Research Discoveries) Award attracted submissions...affected by AHC suffer from recurrent attacks of unilateral paralysis (hemiplegia) that are often painful...stressors, including emotional stress or changes in temperature.  “The disease was first described in 1971,” ...elucidating association between SOX10 and WS4 and also several other rare genetic diseases. We hope kids in Thailand...

  3. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...outcrossed knock-in ready for experimentation. In 2014, several manuscripts published in Genetics detailed approaches... DNA as repair templates which reduces time to generate edits to only four days (Paix et al., 2017). The... demonstrated that selection for repair of a temperature-sensitive pha-1 mutation significantly enriches...design The Meyer lab meticulously characterized several aspects of editing. First, they devised guidelines...modifying the 5’ modification of oligos used to generate linear dsDNA repair templates by PCR boosted efficiency...from a single injected animal.  PAM sequence considerations For changes 5’ to the PAM, sequences corresponding...elegans The Meyer and Mello labs also identified several experimental set ups that could improve CRISPR ...

  4. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...part of my job, in addition to preparing DNA and generating/replenishing kits, is to find new ways to make...extract libraries from previously fragmented DNA.The general process for library preparation using this kit ...total of about 3 hours. It was robust, capable of generating a wide range of insert sizes and also produced...participants from different biotech companies to interact and learn more about one another. They also provided...representatives. I got to talk with a technician from Neon Therapeutics, a biotechnology company that uses neoantigen...extremely helpful since she was familiar with library generation. Also, I now know what to expect at a workshop...

  5. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...2012). The polarizing impact of science literacy and numeracy on perceived climate change risks. Nature... between what I was doing in the lab and the interactions I was having outside of the “ivory tower.” I... acute “adeolitis” – received the best ratings overall, simply because none of the participants had preconceived...show that people with greater levels of science literacy are especially prone to this, possibly because...Fischhoff, B. (2017). Individuals with greater science literacy and education have more polarized beliefs on controversial...

  6. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...for a higher number of MPE. Therapeutic potential  To explore the therapeutic potential of the DAP array...pathogen. Over the past decade, CRISPR tools have leveraged such host-defense mechanisms to enable multiplex...to develop a strategy that would independently generate multiple gRNAs for nCas9-BE, or any other kinds...drive-and-process.” To optimize DAP use, they developed a generalizable method to acquire the most effective DAP array... demonstrated up to 31-loci MBE with over 50% averaging editing efficiency in human cells. MBE with DAP...

  7. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...remote collaborations Transferable Skills Guide: External collaborations Transferable Skills Guide: Teamwork...how to seek out such an arrangement, and how to operate once in that position. What is a visiting scientist...new placement. Make sure you read any relevant literature for the techniques you will be performing to ...home institute. Discuss rights Do you expect to generate data that will contribute to a publication? A ...authorship and rights for anything that could be generated during the visit. If you don’t feel comfortable...

  8. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...development of useful diagnostic methods and improved therapies for PZA-resistant Mycobacteria tuberculosis. Investigation...to tag individual strains of bacteria. Their interactions and growth within the biofilms will then be ... taraxacifolia leaves are widely used to treat several diseases. Her goal is to identify and isolate pure...Therefore, understanding neuronal networks, their interactions and information processing during this condition... to get new insights into functional network interactions. Combining synthetic biology with engineering...resources and microfabrication technologies to generate a self renewable sensor, a bacterium that can ...

  9. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...synthetic) pFZ19 (yellow flame) Temperature inducible promoters Temperature sensitive expression systems ...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...Types of inducible promoters Chemical agents, temperature, and light are all examples of factors that can...promoters; they show near-zero expression at regular temperatures but can be induced by heat or cold exposure....since expression can be repressed by glucose. Temperature inducible promoters are also known for their ...

  10. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ... these interactions, and can give valuable insight into DNA expression and protein interactions, including...technique that provides insight into protein:DNA interactions. ChIP works by using antibodies to capture protein... focuses on the DNA your protein is actively interacting with, reducing background information. Your options...could mean needing to increase crosslinking to counteract the effects of increased sample agitation; a ... this investment can well be worth it once you generate your data. However, it may be a good idea to add...

  11. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...more effectively used in a technology or as a therapeutic.  A (very) brief history GCE came onto the synthetic...specific to the ncAA and orthogonal to (i.e. non-interacting with) the cells’ own natural translation machinery...system (including media, cell type, and growth temperature) may need to be individually optimized for each...focused on turning GCE tools developed in the literature into robust and universal techniques that researchers...fulfill their mission. While they’ve shortlisted several protocols, including their phosphoserine ncAA protocol...., Griffith, M. C., & Schultz, P. G. (1989). A General Method for Site-specific Incorporation of Unnatural...

  12. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ... systems that measure biological phenomena by generating an output which is directly proportionate to ...antibody, enzyme, DNA, etc. The receptors job is to generate a quantitative signal upon recognizing the analyte... (fluorescence, pH, etc.). The transducer will generate a numerical value which represents the amount ...disciplines! Most of the sensors designed for calcium generate a fluorescent output, which will turn on, change... It’s a good thing, though! Viral delivery has several advantages over other mechanisms. For one, many...permanently integrate and/or express long term (up to several years!). Biosensors tend to be more reproducible...

  13. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ... In each case, we leveraged the team’s knowledge of the scientific domain in general and of our data in... that we needed to improve more than that: the general “findability” of the information on the site. Different...approached the issue from multiple angles: the overall user experience of our website, the different website...resources and handle millions of queries. In an iterative process driven by our user experience experts,...approach much more than if we were dealing with general purpose information. Below are a few of the strategies...

  14. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...proteomics--the study of the distribution and interactions of proteins within these structures. Enzyme-...substrate to map the spatiotemporal distribution and interaction networks of specific proteins in living cells...process is much slower and can take as long as several days. In 2015, the laboratory of long-time Addgene..., the enzymes function across a much broader temperature range than BioID, which only functions optimally...modular domains that facilitate protein-protein interactions and mediate biochemical processes. One of the...than previously thought. Sidhu’s group has made several hundred SH3 domain containing plasmids available...

  15. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...interest, as it is prone to generating indel errors. Indel errors generated in the course of repair by ...vast toolbox of processing factors, including polymerases (Pol μ and Pol λ), nucleases (Artemis), and structure-specific...illustrated below, NHEJ-mediated repair of Cas9-generated breaks is useful if the intent is to make a knockout...overhangs is available. Cas9 can also be altered to generate a targeted single strand break; when two such ...Chromosomal Translocations in Human Cells Are Generated by Canonical Nonhomologous End-Joining. Molecular...

  16. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...PubMed PMID:29483642. Making protein-protein interaction studies easier with a 2in1 vector cloning system...cloning system is well suited for protein-protein interaction (PPI) studies in plants that rely on transient...Grefen Lab has utilized the 2in1 cloning system to generate a series of plasmids to study PPI using Förster...Plasmid Toolkit consists of 4 plasmid toolkits generated with 4 different, binary 2in1 vectors with all...powerful approach to control protein-protein interactions in cells because the protein binders can be ... that these photo-reversible protein-protein interactions can control subcellular localization processes...

  17. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    Published
    Feb. 28, 2019, 1:34 p.m.
    ... moment that Teytelman realized that running interactive workshops to train researchers on tools and best...next steps. April Clyburne-Sherin had already run several reproducibility workshops as Director of Scientific...to this open science collaboration. The first iteration of these workshops took place at Caltech in Jan...being adopted by a wider group of researchers. Generating material to empower others to practice and teach...team was assembled when it was realized that interactive workshops that train and provide researchers ...on the Addgene blog: Check out this guide on Transferable Skills: External Collaborations Learn about ...

  18. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...way they are, the research antibodies in your refrigerator often need a little help to be useful. Mainly...enzymes. The most common enzyme conjugates are Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP...This chemical coupling can be achieved through several different mechanisms. Most take advantage of chemical...sequence or that rely on strong protein-protein interactions with other peptides, like an Fc-binding domain...acids or peptide tags. 5) Strong protein-protein interactions with other peptides. Created with BioRender....Ligation Assay for Detecting Protein-Protein Interactions and Protein Modifications in Cells and Tissues...

  19. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...a high imidazole buffer that outcompetes the interaction between the protein of interest and the metal...polyhistidine tagged protein of interest (purple) and several contaminating proteins (blue, green, orange) flows... low imidazole wash buffer disrupts the weak interaction of the contaminating protein but not that of ...A high imidazole elution buffer disrupts the interaction of the protein of interest and it is successfully...protein conformation, the polyhistidine-metal ion interaction is not disrupted by denaturants (Hochuli, 1988... of a protein. Neither the N or C terminus is preferable over the other however, tag placement can affect...

  20. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ... only for translational research, such as gene therapy, but also basic research. To improve cell-type ... with high rates of labeling are ideal of gene therapy applications, promoters that produce sparse labeling...makes it easier to trace a neuron’s projections. Generating animal models: Transgenic animals allow for the...tissues, but they are costly and time-consuming to generate, especially for large animals such as primates...basic as well as translational research for gene therapy. The Roska lab hopes to design improved versions...blog Read more about using AAV for retinal gene therapy Find more about precise neuronal targeting using...
Showing: 341 - 360 of 830 results