We narrowed to 370 results for: Numb

1. Treating Muscular Dystrophy with CRISPR Gene Editing

   Type

   Blog Post

   Published

   Jan. 26, 2016, 3:30 p.m.

   ...excited. This approach may also be applicable to a number of rare genetic diseases caused by splicing defects...

2. How to Negotiate a Successful Lab Start Up Budget

   Type

   Blog Post

   Published

   Sept. 1, 2015, 2:30 p.m.

   ...problem is that the discussions usually focus on the numbers, rather than the necessary resources. This is why...

3. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

   Type

   Blog Post

   Published

   May 18, 2018, 7:32 p.m.

   ...Biology is messy enough that by chance, some small number of the scientists will get a result that confirms...

4. How to Design Your gRNA for CRISPR Genome Editing

   Type

   Blog Post

   Published

   Sept. 24, 2020, 1:15 p.m.

   ...prediction is ever perfect, but this can decrease the number of guides one needs to test in the lab. Importantly...

5. CRISPR 101: Epigenetics and Editing the Epigenome

   Type

   Blog Post

   Published

   June 24, 2020, 5:45 p.m.

   ...loci is possible using CRISPR, and Addgene has a number of tools for this purpose. Epigenetics began as...

6. Hot Plasmids: Spring 2025

   Type

   Blog Post

   Published

   June 3, 2025, 1:15 p.m.

   ... is challenging to monitor because only a small number of glutamate molecules are released in each synaptic...

7. Antibodies 101: The Basics of Western Blotting

   Type

   Blog Post

   Published

   March 8, 2021, 2:41 p.m.

   ...treatment.  Westerns are also a useful readout for a number of cellular assays. They can determine protein ...

8. Antibodies 101: Multiplex Immunofluorescence

   Type

   Blog Post

   Published

   May 23, 2023, 1:15 p.m.

   ...is expressed in the hippocampus, or counting the number of puncta in each cell. When making figures for...

9. Plasmids 101: Repressible Promoters

   Type

   Blog Post

   Published

   Dec. 20, 2022, 2:15 p.m.

   ... repressible binary systems. In recent years, a number of more complex systems have also been developed...

10. Technique: Probe Phage Genomes for Host Binding Proteins

    Type

    Blog Post

    Published

    Sept. 29, 2016, 2:30 p.m.

    ...would for a Western blot in order to reduce the number of cells that will bind non-specifically to either...

11. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type

    Blog Post

    Published

    July 28, 2016, 2:30 p.m.

    ...target states are only represented in a very small number of registers. Instead, one may use qPCR to determine...

12. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type

    Blog Post

    Published

    Oct. 17, 2019, 12:28 p.m.

    ...ions across the axon's membrane: once a certain number of positive ions crosses the cell membrane, a threshold...

13. 9 tips for a successful postdoctoral experience

    Type

    Blog Post

    Published

    Oct. 9, 2018, 12:21 p.m.

    ...Institute. Today's postdoctoral fellows (PFs) face a number of challenges ranging from long periods of training...

14. Transferable Skills Guide: Teamwork

    Type

    Blog Post

    Published

    Nov. 28, 2017, 2 p.m.

    ...communication options. Addgene, along with an increasing number of academic labs, uses Slack constantly. Whether...

15. Quick Guide to All Things Lentivirus

    Type

    Blog Post

    Published

    March 21, 2017, 2:30 p.m.

    ...FACS dosing method correlates viral titre to the number of cells transduced by a GFP encoded virus, and...

16. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type

    Blog Post

    Published

    Dec. 15, 2016, 3:57 p.m.

    ...Bacterial strain with integrated defective prophage A number of E. coli strains exist that stably express lambda...

17. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    Published

    June 28, 2018, 3:01 p.m.

    ...efficiency in the transfected cells, we count the number of tag-positive cells (tag ICC) as a percentage...

18. With an Eye Towards the Future, We Look Back at the March for Science

    Type

    Blog Post

    Published

    Oct. 24, 2017, 1:54 p.m.

    ...few years, there's been a disturbing trend in the number of high profile people denying science, from celebrities...

19. Custom CRISPR Screens & the Green Listed Software

    Type

    Blog Post

    Published

    July 11, 2017, 2:30 p.m.

    .... For example, the analysis becomes easier, the number of cells needed is lower, and perhaps most important...

20. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type

    Blog Post

    Published

    April 25, 2017, 2:30 p.m.

    ...super-resolution imaging, they still do not emit the large number of photons achievable with photoswitchable dyes...