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We narrowed to 610 results for: REV

Showing: 341 - 360 of 610 results

  1. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ... you will not be a graduate student or postdoc forever, and you need to be thinking about what comes next... development center, if one exists. Get online! Review company and institution career pages. Make yourself...

  2. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...takes on cases requiring in-depth analysis, data review, in-house testing etc. Technical support roles ...too commercial or “sales-y”. The work includes reviewing customer data sets, troubleshooting protocols,...

  3. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...company you currently use. You can also find many previously validated gRNAs at Addgene. In vitro screening... and stuff ups). This is a huge improvement on previous mouse work, which could take 8 months or more....

  4. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...subcultured at densities that are low enough to prevent confluency but high enough to promote cell growth...Incidence, sources, effects, detection, elimination, prevention.” Cytotechnology 39.2 (2002). 75-90. Pubmed PMID...

  5. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...seizures or some other unwanted phenomenon or it could reveal something fascinating. Behavior is based primarily...behavioral test might be contextual fear conditioning (reviewed in [4]). Contextual fear conditioning is a behavioral...

  6. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...co-opted toward that aim (i.e. sacB). Importantly, to prevent activation of toxins before the second crossover... in 5 days. Counter-selection with sacB has revolutionized allelic exchange. Even now, only half our lab...

  8. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...encoded tag and exogenously added components. For a review see Lotze et al. (2016). To date, small probes ... tools engineered from phytochromes." Chemical reviews 117.9 (2017): 6423-6446. PubMed PMID: 28401765....

  9. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...operator is in a different room.   Design: The previous cycle made me realize we needed to focus on ways...wouldn’t be in there with them. I knew from my previous cycles that I wanted to be able to talk to people...

  10. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...antibody dilution — both factors we’ve covered in our previous blog posts (1, 2) on western blotting. If the ...confidence in the accuracy of western blots. Expert Review of Proteomics, 11(5), 549–560. https://doi.org/...

  12. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...strategies. As an example, we’ll use one of my previous experiments where I harvested a melanoma tumor...single cell + live” population, acquired from the previous gating pipeline, about 53% of my cells are dendritic...

  13. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...CRISPR editing CRISPR, of course, has started a revolution in gene editing. However, while CRISPR is remarkably...CRISPR-based approaches is described in several recent reviews (Howes and Schofield, 2015; Moser and Hirsch, 2016...

  14. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...expensive sequencing fuels a sequencing revolution that still prevails. Sequencing whole genomes or profiling...

  15. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...expressed for a short period of time, which would prevent the accumulation of unwanted mutations over time...upregulating HDR, NHEJ must be downregulated to prevent the introduction of new mutations by CRISPR; editing...

  16. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...your success. Therefore, it only makes sense to reverse engineer your budgetary needs from your experimental...going through some of the questions we mentioned previously.  There is value in the process of creating a...

  17. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...retrotransposon insertion, a combined endonuclease-reverse transcriptase uses homology arms flanking an RNA...repair (HDR), and insertion is scarless, unlike previous integrase-based approaches. Find STITCHR plasmids...

  18. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    .... Of note, most of these optimized Cas enzymes prevent cutting at mismatched guide sites, but not necessarily...alternative pathways. See (Guo et al, 2023) for a review of other targeted sequencing methods. Candidate...

  19. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...an alternative but well-studied kinase known to prevent tau hyperphosphorylation. You could run brain samples...means an exhaustive list and we encourage you to review the literature for your specific experimental technique...
Showing: 341 - 360 of 610 results