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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 609 results for: cas

Showing: 341 - 360 of 609 results

  1. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at...Viral Transductions Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect...

  3. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...positively affect scientific research. As Maria Castro of University of Michigan says, “Addgene has transformed... helped accelerate scientific discoveries.” Occasionally, a plasmid tool is so influential that it is...

  4. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...genes at the site of insertion. In both of these cases, transposons combine the advantages of viruses and...2) a transposon containing the gene-expression cassette that can translocate within the genome. The Sleeping...

  5. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ... the other posts in this series. Listen to our podcast interview with Connie Cepko to learn about her ...leaders rarely give orders (although they must occasionally). Good leaders exert influence in other ways...

  6. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ... a PI who is a good mentor is important in both cases. As a PhD student you need to learn how to be a ...you pursue any career alternative? Listen to our podcast interview with Harvard Medical School researcher...

  7. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...non-specific staining can be a challenge in the best of cases. But it can be particularly challenging when your...primary antibody that has your reporter (in this case a fluorophore) directly attached to it. (B) Another...

  8. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ... is not suitable for use in apoptotic cells as Caspase3/7 both cleave after the DVPD sequence. Check out...protein functionality (Arribere, et al). In rare cases, tags can also affect protein localization and solubility...

  9. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...transgene free individual. For example, with CRISPR-Cas9 gene editing, transformation may be identified by...CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 Tips for Arabidopsis Transformation CRISPR 101: ...

  12. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...of a hairpin structure within your primer. In our case, we will add TAAGCA, resulting in a final Forward...to the sequence to be amplified (the ORF in this case), not the Tm of the entire primer. If you are amplifying...

  13. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...computer modeling and predictive algorithms to forecast the drug epitopes likely to be presented by T ...between 2D assays and in vivo animal models. In these cases, they are designed to mimic the human lymphatic ...

  15. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ... strategic knowledge to solve a clinical disease case. As a teacher, you explain the game rules and then...face each other on a single chosen clinical disease case. Then, each team received a whole set of cards (...

  16. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...really matters. Well, it turns out this isn’t the case for using AAV. When it comes to infecting cells,... delivering receptor constructs with AAV. In the case of channelrhodopsins, fewer channel proteins are...

  17. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ... common event. What are exosome AAVs? In 2012, Casey Maguire and colleagues from Massachusetts General...28082074. PubMed Central PMCID: PMC5368844.  Maguire, Casey A., et al. "Microvesicle-associated AAV vector as...

  18. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...we all shared a passion for DIY Biology and, on occasion, would meet in the same space where BosLab exists...journal club discussion on Kevin Esvelt’s CRISPR/Cas9 gene drive paper was very popular. People kept asking...

  19. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ... start of the CRISPR craze, but he says, “In the case of CRISPR, the hype was completely justified… it...the audio engineer and producer for the Addgene Podcast.       Resources at Addgene Find CRISPR resources...

  20. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...of having all the pages in a Curricula Vitae to showcase your publications, academic performances, and ...related to the job you are applying for, in which case talk about your research, as an understanding of...
Showing: 341 - 360 of 609 results