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We narrowed to 715 results for: cas

Showing: 1 - 20 of 715 results

  1. CRISPR Plasmids - Cascade-Cas3

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    Collection
    ...still separate the individual Cas components. Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com... CRISPR-Cas and Cascade for large genomic deletions. CRISPR...comprised of a combination of Cas5, Cas6, Cas7, Cas8, and Cas11. Cascade is directed to target DNA using... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3 Browse ...recruits Cas3. After nicking the non-targeted strand, Cas3 starts unraveling DNA using its helicase properties...this, some CRISPR-Cas3 plasmids take an all-in-one approach and include Cas3 and Cascade on one single plasmid...Blog Posts Cas3 is a unique member of the CRISPR family. After nicking the target DNA, Cas3 initiates ...

  2. Plan Your Experiment

    Type
    Collection
    ...utilized for CRISPR/Cas gene editing. This image summarizes ways that the CRISPR/Cas system can be delivered...target cells translate the mRNA encoding Cas to produce the Cas enzyme, and the enzyme then forms a complex...directly combine purified Cas protein and in vitro transcribed gRNA to form a Cas-gRNA complex. You then ... joining (NHEJ) at the cut site Cas9 or Cas9 nickase High-fidelity Cas enzymes increase specificity Dual-nickase...components (Cas enzyme and guide RNA) for your experiment (Figure 1). You will decide how to express Cas9, the...generally only compatible with smaller Cas enzymes, like S. aureus Cas9. For more information, see our blog...deliver Cas-gRNA ribonucleoprotein (RNP) complexes directly into your target cells. In this case, your ...

  3. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID ...Drosophila and other insects. Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB... Gene/Insert Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant, you will need two...When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in the same cell...time. Single plasmids containing both the gRNA and Cas protein act as all-in-one vectors, but their function...Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 49410...

  4. CRISPR History and Development for Genome Engineering

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    Collection
    ...prevent the CRISPR-Cas complex from binding to DNA. Others inhibit the recruitment of Cas proteins to the...allowing multiple Cas9s to be used together. Researchers have engineered mutant Cas enzymes with alternative... Fu Y, Sander JD, Reyon D, Cascio VM, Joung JK. 2014. Improving CRISPR-Cas nuclease specificity using ...immune system - utilizing various CRISPR-associated (Cas) genes to not only store a record of invading phages...upon re-exposure. More specifically, specialized Cas proteins snip foreign DNA into small fragments approximately...stretches of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci... Through sequence homology, these crRNAs guide a Cas nuclease to the specified exogenous genetic material...

  5. CRISPR Plasmids - Yeast

    Type
    Collection
    ...that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID ...designed for use in yeast. Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB...Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant, you will need two...When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in the same cell...time. Single plasmids containing both the gRNA and Cas protein act as all-in-one vectors, but their function...PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...

  6. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID ...for use in zebrafish. Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB...When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in the same cell...time. Single plasmids containing both the gRNA and Cas protein act as all-in-one vectors, but their function...Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 46759...none, need Cas9 plasmid Chen and Wente 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  7. CRISPR Plasmids - CRISPR Transposases (CAST)

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    Collection
    ...two main classes of CASTs are type I and type V-K. Type I systems use a type I Cas effector, composed ...composed of several separate Cas enzymes that form a complex called Cascade ( C RISPR- as sociated c omplex for...target site. Type V-K systems use a type V-K Cas effector, Cas12k, and three transposase components (TnsB,...transposases (Tns) with the precise DNA-targeting of CRISPR-Cas systems, allowing for the integration of large fragments...fragments of DNA at specific locations. The Cas effector lacks nuclease activity, instead recruiting the... CRISPR CASTs CRISPR Plasmids: CRISPR Transposases (CASTs) Browse...transposases (CASTs) for insertion of donor DNA. CRISPR...

  8. Gibson Assembly Protocol

    Type
    Protocol
    ...Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO. (2009). Enzymatic assembly of DNA molecules...opens in a new window) Gibson DG, Smith HO, Hutchison CA, Venter JC, Merryman C. (2010). Chemical synthesis...

  9. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID ...for use in C. elegans. Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB...When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in the same cell...time. Single plasmids containing both the gRNA and Cas protein act as all-in-one vectors, but their function...Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 47549 pDD162 (Peft-3::Cas9 + Empty ...need Cas9 plasmid Joung 46170 PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection none, need Cas9 plasmid... none, need Cas9 plasmid Boxem 47941 pMB60 T7 BsaI In vitro transcription none, need Cas9 plasmid Boxem...

  10. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID ...for use in bacteria. Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB.... ID Plasmid Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant, you will need two...When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in the same cell...time. Single plasmids containing both the gRNA and Cas protein act as all-in-one vectors, but their function...PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...

  11. CRISPR Guide

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    Collection
    ...type I Cas effector, composed of several separate Cas enzymes that form a complex called Cascade (see Cas3...Base Editing with CRISPR CasPEDIA: A Functional Classification of Cas Enzymes Cas Enzymes and PAM Sequences...application. Cas9 Orthologs While SpCas9 is the most commonly used Cas enzyme, additional Cas9 orthologs ...editing with Cas3 Browse Plasmids: Cascade-Cas3 Want more information on the wide variety of Cas enzymes? ...from. Many variations of Cas9 exist, along with other Cas enzymes like Cas12a, which are available for...paired with the appropriate Cas enzyme. Specific enzymes, such as Cas12a or Cas13 , can improve multiplexing...Other natural Cas orthologs include Hsp1Cas9 (from Helicobacter sp. MIT 11-5569 ) and Nme2Cas9 (from Neisseria...

  12. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. Do ... expression systems. Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB...Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant, you will need two...When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in the same cell...time. Single plasmids containing both the gRNA and Cas protein act as all-in-one vectors, but their function...PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...

  13. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID ...When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in the same cell...time. Single plasmids containing both the gRNA and Cas protein act as all-in-one vectors, but their function...Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 51306...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...designed for use in Xenopus. Cut Fully functional Cas9 enzymes designed to introduce a double-strand break...surrounding the DSB is introduced along with the Cas9 and gRNA plasmids, the cell may instead repair the...

  14. CRISPR Plasmids - Plants

    Type
    Collection
    ...that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids. ID ...designed for use in plants. Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB...Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant, you will need two...When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in the same cell...time. Single plasmids containing both the gRNA and Cas protein act as all-in-one vectors, but their function...PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...

  15. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems...Protocols gRNA Design Tools CRISPR Blog Posts CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant, you will need two...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  16. CRISPR References and Information

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    Collection
    ... range of Cas enzymes (Cas9 and Cas12) and organisms. Developed by the Jin-Soo Kim Lab . Cas-Designer ...probability of off-target effects. Cas-Designer integrates information from Cas-OFFinder and Microhomology predictor...design. The current version supports SpCas9, SpCas9-VQR, and enAsCas12a Cas enzymes. Developed by the Manuel...National Laboratory at the National Cancer Institute. Cas-OFFinder (Link opens in a new window) Identifies ...a new window) Labs. Off-Spotter: tool for CRISPR/Cas design (Link opens in a new window) Program for designing... S. pyogenes Cas9, S. aureus Cas9, S. thermophilus Cas9, N. meningitidis Cas9, or Cas12a from your input...vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H Cas9 ; p201N Cas9 ; pUC gRNA Shuttle PDF, 500 KB...

  17. CRISPR Plasmids - Tagging

    Type
    Collection
    ...proteins. This CRISPR/Cas plasmid-system consists of two components: A vector containing Cas9 and a validated...marker. The first deposited plasmids in this CRISPR-Cas tagging system were tested by tagging transcription...into human FBL locus Jorgensen Lab SapTrap CRISPR/Cas Toolkit SapTrap is a modular toolkit from Erik Jorgensen's...GFP targeting vector. Jorgensen Lab SapTrap CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible Degron Tagging...Kanemaki's lab has developed a simple and scalable CRISPR/Cas-based method to tag endogenous proteins by using ...Auxin-inducible degron (AID) tagging with CRISPR/Cas to generate conditional alleles of essential nuclear...Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which expresses an untagged Cas9 AND a gRNA...

  18. CRISPR Plasmids - dCas9-FokI

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    Collection
    ... CRISPR dCas9-FokI CRISPR: dCas9-FokI...Tools CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes...with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...gRNAs, binding ~15-25 bp apart, are required for dCas9-FokI to dimerize at a given region of the genome...

  19. CRISPR Plasmids and Resources

    Type
    Collection
    ...Engineering Cut Wild-type Cas enzymes generate double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate... generate single-strand DNA breaks. Prime Edit Cas nickase enzymes fused to reverse transcriptase install... without promoting DSB formation. Delete NEW Cascade-Cas3 generates large genomic deletions. Insert NEW...Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused...repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin..., not DNA, across multiple systems. RNA Editing Cas13 enzymes fused to deaminases can edit bases in RNA...powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used to isolate a...

  20. Rinehart Lab Phosphoprotein Reagents

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    Collection
    ...for using iSPI, rEcoli XPS, and pSerOTS-C1* (V70) (ca. 2022) from the Rinehart lab: Methods for iSPI, rEcoli...29920078 (Link opens in a new window) Improved Reagents (ca. 2015) described in: Pirman, N. L., Barber, K. W....window) Protocol for using the Improved Reagents (ca. 2015) from the Rinehart lab: Users Guide for Improved...Phosphoprotein Reagents (DOCX, 525.9 KB) Reagents (ca. 2014) described in: Heinemann, I. U., Rovner, A....Link opens in a new window) Protocol for Reagents (ca. 2014) from the Rinehart lab: Users Guide for 2014...Phosphoprotein Reagents (PDF, 691.3 KB) Original Kit (ca. 2011) described in: Park, H., Hohn, M. J., Umehara...
Showing: 1 - 20 of 715 results