We narrowed to 365 results for: erg
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TypeBlog PostPublishedJune 21, 2016, 2:30 p.m....at Harvard University currently studying the serotonergic system and behavior. References 1. Southern...
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How to Negotiate a Successful Lab Start Up Budget
TypeBlog PostPublishedSept. 1, 2015, 2:30 p.m....before hand, you’re saving the institute time and energy that would typically be wasted going through some... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog PostPublishedApril 24, 2015, 2:39 p.m....-1.0 expresses RFP when the construct has not undergone recombination. This default state was disproportionately... -
Antibodies 101: The Basics of Western Blotting
TypeBlog PostPublishedMarch 8, 2021, 2:41 p.m....wet transfer system the sandwich is completely submerged in a tank filled with a transfer buffer formulated... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog PostPublishedJune 24, 2020, 5:45 p.m....publication at Addgene. McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, Kramer A, Martens A, Edwards... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog PostPublishedJan. 16, 2025, 3:42 p.m....from AAV2 and engineered to infect neurons and undergo retrograde transport, traveling up the axon to ... -
A Control for All Seasons
TypeBlog PostPublishedOct. 5, 2023, 1:15 p.m....identical protocols that the experimental samples undergo. If the antibody is specific, then the target signal... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m....you can use Color>Split Channels and then Color>Merge Channels into whatever combination of colors you... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog PostPublishedJuly 12, 2016, 2:30 p.m....Goodwin LO, Morgan JL, Juan WC, Roca X, Ong ST, Bergstrom DE (2018) Viable Mice with Extensive Gene Humanization... -
Plasmids 101: Repressible Promoters
TypeBlog PostPublishedDec. 20, 2022, 2:15 p.m.....89.12.5547. PMID: 1319065; PMCID: PMC49329. Triezenberg SJ, Kingsbury RC, McKnight SL. Functional dissection... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog PostPublishedMay 31, 2016, 3:30 p.m....exonic regions. If the cell’s chromatin structure undergoes significant rearrangement (such as when a stem... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog PostPublishedNov. 2, 2021, 1:15 p.m....AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH (2018) An Alternate Route for Adeno-associated... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog PostPublishedOct. 8, 2020, 1:15 p.m....Biotechnology. References Degeling MH, Maguire CA, Bovenberg MSS, Tannous BA (2012) Sensitive Assay for Mycoplasma... -
Transferable Skills Guide: Teamwork
TypeBlog PostPublishedNov. 28, 2017, 2 p.m....experience on a successful scientific team came as an undergrad at Worcester Polytechnic Institute. Though some... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog PostPublishedMarch 7, 2017, 3:30 p.m....CRISPR-Cas9. Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog PostPublishedApril 16, 2024, 1:15 p.m.... your own ligands brings up bad memories of undergraduate organic chemistry lab, don’t worry – many SNAP-tag... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog PostPublishedDec. 17, 2020, 1:30 p.m....professor at the University of California, Santa Cruz. Emerging CRISPR/Cas9 editing technologies have transformed... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog PostPublishedApril 25, 2017, 2:30 p.m.... FP-protein fusions (FP-chimeras) is that they undergo normal protein turnover. FP-chimeras are continuously... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog PostPublishedMarch 14, 2017, 2:30 p.m....elimination by T cells. Immunotherapy strategies that synergize with these positive impacts on the immune system... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog PostPublishedMay 14, 2024, 1:15 p.m....Modular Vector Assembly Enables Rapid Assessment of Emerging CRISPR Technologies. Cold Spring Harbor Laboratory...