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Showing: 341 - 360 of 704 results

  1. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...This post was contributed by guest blogger Stephanie Hays, a researcher at the University of California...expected. Jennifer Stancill, Ph.D., is currently a post-doctoral fellow at Vanderbilt University. She studies... Resources on the Addgene Blog Read Our Previow Post on the March for Science Learn How Future of Research...chair of the postdoctoral engagement committee and active member of the university's Postdoctoral Association...in the veracity of its work. Alina Chan is a postdoctoral researcher at Harvard Medical School working...runs hand in hand with policy. Alexandra is a postdoctoral researcher in the University of Michigan Department...continue the dialogue. Erin Golden is currently a postdoctoral fellow at the University of Colorado Anschutz...

  2. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...Jan 27, 2015 and last updated Dec 17, 2020. This post was contributed by Jordan Ward who is an assistant...the majority of knock-ins were occurring 24 hours post-injection, making screening for knock-ins much more..., some of the “elegans” methods outlined in this post – particularly the use of co-selection methods –...

  3. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...Here THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED...second chance” mechanism. To prevent new editing post-HDR, repair templates should remove the PAM sequence...1053 aa/3.2 kb) with AAV vectors for efficient postnatal editing. LbCpf1 and AsCpf1 are 3.7 kb and 3.9 ...

  4. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...This guest post was contributed by Sana Khan Khilji, a PhD student at the Max Planck Institute of Colloids...progress.Learn more about cell culture contamination in this post from the ATCC Declutter! Keep the hood clean Hoods...

  7. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...into play? We’ll dive into those questions in this post as we explore… the life and times of the myc tag...substance P has an amide group added to its C-terminus post-translationally, which turned out to be critical...

  8. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...make the most of these pages, we've written this post to guide you through them and make the best use ...publications which have used the plasmid (see our blog post) for more information about this feature!).  This...

  9. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...This post was contributed by Gal Haimovich of greenfluorescentblog. Be honest. Do you really know how... and their relevant mutations, check out Marcy's post from last week on GFP. Additionally, many other ...

  11. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ... including plants, mammals, and insects. In this post, we will compare two of the most widely used systems... time, or reagents and equipment. Hopefully this post has provided some insight on these differences in...

  12. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...cells, but mammalian cells can perform human-like post-translational modifications that bacterial and most...effective compared to those aliquots frozen immediately post-harvest. Stress-tested antibodies that are not equally...

  13. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...This post was contributed by guest blogger, Luke Lavis, a Group Leader at the Janelia Research Campus... blogger, Luke D. Lavis! All images in this blog post were contributed by Luke D. Lavis. Luke D. Lavis...

  15. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ... Thisted, Thomas, and Kenn Gerdes. "Mechanism of post-segregational killing by the hok/sok system of plasmid...to efficient cloning Read all Plasmids 101 blog posts Read about knockout/knock-in plasmids Resources...

  16. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...experiment, they definitely made my life easier!  In this post, I’ll share some AAV production, purification, and...account for batch-to-batch variability. Check out this post to learn more about different ways to titer AAV....

  17. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...Additional Resources on the Addgene Blog Read this blog post about CRISPR Kinome Libraries available at Addgene...for large-scale functional screens Read this blog post about genome-wide screening using CRISPR Resources...

  18. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ... and memory. We’ll cover these in a  future blog post, so stay tuned!  References Alpár, Alán, et al...Addgene blog Read all of our viral vectors blog posts Find tips for getting started with viral vectors...

  19. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...CRISPR blog posts Download our CRISPR cheat sheet Read our science communication blog posts Resources ...past 20 years has been aimed at grad students, postdocs, and other working scientists, creating a presentation...

  20. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...This post was contributed by Patrick Miller-Rhodes, a Ruth L. Kirschstein NRSA Predoctoral Fellow at ...resources on the Addgene blog Browse our CRISPR blog posts Download our CRISPR cheat sheet Download our CRISPR...
Showing: 341 - 360 of 704 results