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Showing: 341 - 360 of 694 results

  3. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...into play? We’ll dive into those questions in this post as we explore… the life and times of the myc tag...substance P has an amide group added to its C-terminus post-translationally, which turned out to be critical...

  4. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...Here THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED...second chance” mechanism. To prevent new editing post-HDR, repair templates should remove the PAM sequence...1053 aa/3.2 kb) with AAV vectors for efficient postnatal editing. LbCpf1 and AsCpf1 are 3.7 kb and 3.9 ...

  5. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...make the most of these pages, we've written this post to guide you through them and make the best use ...publications which have used the plasmid (see our blog post) for more information about this feature!).  This...

  6. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...This post was contributed by Gal Haimovich of greenfluorescentblog. Be honest. Do you really know how... and their relevant mutations, check out Marcy's post from last week on GFP. Additionally, many other ...

  7. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ... and memory. We’ll cover these in a  future blog post, so stay tuned!  References Alpár, Alán, et al...Addgene blog Read all of our viral vectors blog posts Find tips for getting started with viral vectors...

  8. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...CRISPR blog posts Download our CRISPR cheat sheet Read our science communication blog posts Resources ...past 20 years has been aimed at grad students, postdocs, and other working scientists, creating a presentation...

  9. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...This post was contributed by Patrick Miller-Rhodes, a Ruth L. Kirschstein NRSA Predoctoral Fellow at ...resources on the Addgene blog Browse our CRISPR blog posts Download our CRISPR cheat sheet Download our CRISPR...

  10. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at the Janelia...transferable skills guide Read all of our career blog posts Resources on Addgene.org Learn more about careers...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...and now serves as the Director of Student and Postdoctoral Programs at the Janelia Research Campus of the...

  11. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...This post was contributed by guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia ...the Addgene blog Browse all of our CRISPR blog posts Check out other CRISPR methods for bacteria Resources...

  12. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...please sign up here.  Here's what you'll find in this post: Yeast-based protein evolution Fluorescent tools...from Chertkova et al., 2020. In their BioRxiv post, the authors generated and described several improved...

  13. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...their interaction. You can read our previous blog post for more on BiFC principles and experimental design...resources on the Addgene blog Addgene Blog Biosensors posts Resources on Addgene.org Fluorescent Biosensors...

  14. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...libraries can be quite challenging to use! In this post, I’ll walk you through how to prep and transduce...perform your pooled library screen…but that’s a blog post for another day.  Using pooled libraries can be ...

  15. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...applications from across disciplines now available. In this post, we’ll review the inspiration behind ExM, how it...samples can sometimes take on a fragmented appearance post-expansion, but that doesn’t mean we should start...

  16. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ... including plants, mammals, and insects. In this post, we will compare two of the most widely used systems... time, or reagents and equipment. Hopefully this post has provided some insight on these differences in...

  17. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...cells, but mammalian cells can perform human-like post-translational modifications that bacterial and most...effective compared to those aliquots frozen immediately post-harvest. Stress-tested antibodies that are not equally...

  18. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...This post was contributed by the Mesothelioma Cancer Alliance. We have learned much about the causes ...be protected from this dangerous material. This post was contributed by the Mesothelioma Cancer Alliance...

  19. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...acid purification methods not mentioned in today’s post? Let us know in the comments! Additional resources...protocols and tips Browse our plasmid cloning blog posts Resources on Addgene.org Find protocols for molecular...

  20. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ... blog posts related to viral vector protocols and tips Read all of our viral vectors blog posts Resources...
Showing: 341 - 360 of 694 results