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...Jan 27, 2015 and last updated Dec 17, 2020. This post was contributed by Jordan Ward who is an assistant...the majority of knock-ins were occurring 24 hours post-injection, making screening for knock-ins much more..., some of the “elegans” methods outlined in this post – particularly the use of co-selection methods –...

...This guest post was contributed by Sana Khan Khilji, a PhD student at the Max Planck Institute of Colloids...progress.Learn more about cell culture contamination in this post from the ATCC Declutter! Keep the hood clean Hoods...

...This post was contributed by Greg Dingle, a software engineer with the Chan Zuckerberg Initiative. We...resources on the Addgene blog Read our CRISPR 101 blog posts Learn about some considerations when designing gRNAs...

...also work equally well in dividing and quiescent, post-mitotic mammalian cells. Applications of CRISPR...Optimizing sgRNA Activity by John Doench All CRISPR Posts  ...

...their interaction. You can read our previous blog post for more on BiFC principles and experimental design...resources on the Addgene blog Addgene Blog Biosensors posts Resources on Addgene.org Fluorescent Biosensors...

...into play? We’ll dive into those questions in this post as we explore… the life and times of the myc tag...substance P has an amide group added to its C-terminus post-translationally, which turned out to be critical...

...Here THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED...second chance” mechanism. To prevent new editing post-HDR, repair templates should remove the PAM sequence...1053 aa/3.2 kb) with AAV vectors for efficient postnatal editing. LbCpf1 and AsCpf1 are 3.7 kb and 3.9 ...

...make the most of these pages, we've written this post to guide you through them and make the best use ...publications which have used the plasmid (see our blog post) for more information about this feature!).  This...

...This post was contributed by Gal Haimovich of greenfluorescentblog. Be honest. Do you really know how... and their relevant mutations, check out Marcy's post from last week on GFP. Additionally, many other ...

... Thisted, Thomas, and Kenn Gerdes. "Mechanism of post-segregational killing by the hok/sok system of plasmid...to efficient cloning Read all Plasmids 101 blog posts Read about knockout/knock-in plasmids Resources...

...experiment, they definitely made my life easier!  In this post, I’ll share some AAV production, purification, and...account for batch-to-batch variability. Check out this post to learn more about different ways to titer AAV....

...Additional Resources on the Addgene Blog Read this blog post about CRISPR Kinome Libraries available at Addgene...for large-scale functional screens Read this blog post about genome-wide screening using CRISPR Resources...

... and memory. We’ll cover these in a  future blog post, so stay tuned!  References Alpár, Alán, et al...Addgene blog Read all of our viral vectors blog posts Find tips for getting started with viral vectors...

...CRISPR blog posts Download our CRISPR cheat sheet Read our science communication blog posts Resources ...past 20 years has been aimed at grad students, postdocs, and other working scientists, creating a presentation...

...This post was contributed by Patrick Miller-Rhodes, a Ruth L. Kirschstein NRSA Predoctoral Fellow at ...resources on the Addgene blog Browse our CRISPR blog posts Download our CRISPR cheat sheet Download our CRISPR...

...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at the Janelia...transferable skills guide Read all of our career blog posts Resources on Addgene.org Learn more about careers...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...and now serves as the Director of Student and Postdoctoral Programs at the Janelia Research Campus of the...

... including plants, mammals, and insects. In this post, we will compare two of the most widely used systems... time, or reagents and equipment. Hopefully this post has provided some insight on these differences in...

...cells, but mammalian cells can perform human-like post-translational modifications that bacterial and most...effective compared to those aliquots frozen immediately post-harvest. Stress-tested antibodies that are not equally...

...This post was contributed by guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia ...the Addgene blog Browse all of our CRISPR blog posts Check out other CRISPR methods for bacteria Resources...

...please sign up here.  Here's what you'll find in this post: Yeast-based protein evolution Fluorescent tools...from Chertkova et al., 2020. In their BioRxiv post, the authors generated and described several improved...