...rules are: (1) don’t use the same overhang twice; (2) avoid palindromes; (3) no overhangs with the same...genomes, it required a very stringent selection process and benefited from a longer reaction time of 15...

...al (Nature. 1988 Jul 28;334(6180):320-5.) PubMed. 2. A segment of the 5' nontranslated region of encephalomyocarditis... a novel gene, is not always a straightforward process. Rather than try to directly detect your gene of...

...rate of 10-55% - the PASTE system.     Fig. 2: Schematic of programmable gene insertion with PASTE... a guide RNA. The enzyme has dual activities: processing pre-CRISPR RNA into mature CRISPR RNA and cleaving...

... vivo and in vitro applications.   Figure 2: Modular features of DIRECTED particles where the ... more accessible by simplifying the production process and could even enable the creation of CAR-T cells...

...Nutr, 129 (2009a): 477–484. PubMed PMID: 10064313. 2. Chapman-Smith, A., & Cronan, J. E. “The enzymatic...These biotin dependent enzymes are essential for processes such as gluconeogenesis, lipogenesis, amino acid...energy transduction. Biotinylation is the general process by which biotin is added to a protein or macromolecule...biotinylation occurs naturally in cells and is the process by which biotin is covalently bonded to a specific...

...become a valuable asset yourself.     Fig 2: A diagram of the Thomas-Kilmann Conflict Model, courtesy...communicated. The next step is to come up with a viable process that will avoid such miscommunications in the future...and examples that have guided your resolution processes in the past. Types of Resolution There are of...

...gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS) General protein expression...auxillary plasmids may be isolated during the prepping process. How does inducible expression work? As mentioned...

...Postdoctoral Associations around Boston - From Panel Session 2: Defining the Postdoc: An Institutional Perspective...questions or concerns throughout the publishing process. The diversity panel - Equity and inclusion The...

...signal separately for each target.   Figure 2: Examples of simultaneous multiplex IF labeling of... appropriate secondary antibodies. The general process is similar to standard immunofluorescence, but ...spatial analysis of cells in complex tissues. Proceedings of the National Academy of Sciences, 117(52),...

...of concept for further development. Figure 2: Mechanisms of deaminase-based adenine transversion...editor. Black arrows indicate natural cellular processes. Created with BioRender.com. The first deaminase-free...

...two visible blue or magenta chromoproteins (Figure 2) (Liljeruhm et al., 2018). This system is a nice confirmation... polylinker sequences of molecular vectors". Proceedings of the Belarusian State University Series of ...

..., whereas the mVenus-APT1 fusion does not (figure 2). In the mVenus-APT1, a lipidation motif, which is...circularly permuted yellow fluorescent proteins." Proceedings of the National Academy of Sciences 101.29 (2004...

...excise the HIV-1 genome from every infected cell and 2. prevent reinfection of those cells. Kaminski et al...target sequence. Kaminski et al. next tested their procedure in HIV-1 infected T-cells to see if they could...

...as the protocol remains the same.    Figure 2: Microscopy-based antibody experiments should include...considerably, then the high degree of variability in the process could make it difficult to obtain meaningful results...

...15.4%) 46 (59%) of these students were in the last 2 years of their degree. Students filled in a pre-program...new professional capacity generated during this process result directly in positive impacts on the lives...

...experience in cancer research After graduating with a 2:1 BSc in Molecular Biology (roughly a B average in... controlled. I worked from Standard Operating Procedures (SOP’s) and every item I tested was constantly...the technology is so advanced that most of the procedures in the lab are done with robots. Young scientists...

...Bachelor's degree Generally a short-term position (1-2 years) Often a gap-year position taken to transition...medical practice, and understanding the scientific process can help us understand how new therapies can be...experience. Previous Addgene blogs have discussed the process that goes into choosing a good scientific mentor...

...ask new and interesting questions in your own lab. 2. Seek multiple mentors during your postdoc At the ...career and you'll become a better scientist in the process. 9. Avoid burnout The suggestions in this blog ...a diet rich in vegetables, foods with minimal processing, and low in empty calories will make you feel...

...start selecting and testing systemic capsids! Figure 2 contains a decision tree for selecting a capsid, while... highlighted below the table.     Figure 2: This decision tree can help guide your choice on ...transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt.3440 Goertsen...organs. AAV.CAP-B22 was developed through the same process as AAV.CAP-B10 and shows high transduction levels...

... from damage (Abe & Lim, 2024).    Figure 2: A) When proteins (blue) interact with the air-water... air-water interface (AWI) during the freezing process, resulting in sample loss. Ci Ji Lim’s lab found...