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We narrowed to 373 results for: proc.2

Showing: 341 - 360 of 373 results
  1. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...PMID: 24901218. PubMed Central PMCID: PMC4047077. 2. Pagliara, Patrizia, et al. "Ostreopsis cf. ovata ...bisphenol A-induced perturbation in biological processes in ER-negative HEK293 cells." PLoS One, 2014. ...
  2. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...PMID: 25538079. PubMed Central PMCID: PMC4353587. 2. Stephen, Andrew G., et al. "Dragging ras back in ...from rogue cells when something does go awry. The process of programmed cell death, or apoptosis, is one ...
  3. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...variety of environmental conditions, and double every 2 hours. Yeast are also the first eukaryotic genome ...actually share many biological properties and processes with our own cells. Like human cells, yeast DNA...proteins and tissues to better assess developmental processes. For example, the Stainier lab studied the proliferation...making it ideal to study basic molecular biology processes. C. elegans are also quite amenable to genetic...investigate an array of biological systems and processes including the nervous system, development, cell...β-cells identified by HOTcre mediated labeling." Proceedings of the National Academy of Sciences 106.35 (2009... in freely behaving Caenorhabditis elegans." Proceedings of the National Academy of Sciences 113.8 (2016...
  4. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...PMID: 27151215. PubMed Central PMCID: PMC4938170. 2. Zuris, John A., et al. "Cationic lipid-mediated delivery... to genome editing of mouse zygotes, through a process known as CRISPR-EZ (CRISPR RNP Electroporation ...
  5. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...of these issues are not particularly new. Chapter 2, “The Professional Scientist,” addresses the challenges...consequence of the undermining of the scientific process and the marginalization of science, particularly...
  6. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ... the Cas machinery where to cut.      Fig. 2: Heatmap indicating loci where SNPs reside on sgRNA...with an ancestry-based bias.   The correction process does not stop there. Currently, they are working...
  7. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...community is active, engaged, and has great questions! 2. The r/Science subreddit is well moderated. One of...You can see some of Joanne’s comments on the AMA process below, but we also wanted to share some thoughts...submission to avoid rousing the trolls. 3. The AMA process was simple and well organized. You can check out...
  8. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ... Methods 2009; 6, 343-345. PubMed PMID: 19363495. 2. Wang JW, Wang A, Li K, Wang B, Jin S, Reiser M, Lockey... menu of common cloning options in the deposit process in 2014 because of its gain in popularity. Gibson... the Miller Lab Protocol). The Gibson assembly process can be used to assemble up to 6 fragments in one...time commitment. Another advantage is that this process makes it easy to generate wild type and mutant ...drawback to the Gibson assembly technique is that the process works best with fragments over 200 nucleotides....
  9. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...rules are: (1) don’t use the same overhang twice; (2) avoid palindromes; (3) no overhangs with the same...genomes, it required a very stringent selection process and benefited from a longer reaction time of 15...
  10. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...al (Nature. 1988 Jul 28;334(6180):320-5.) PubMed. 2. A segment of the 5' nontranslated region of encephalomyocarditis... a novel gene, is not always a straightforward process. Rather than try to directly detect your gene of...
  11. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...rate of 10-55% - the PASTE system.     Fig. 2: Schematic of programmable gene insertion with PASTE... a guide RNA. The enzyme has dual activities: processing pre-CRISPR RNA into mature CRISPR RNA and cleaving...
  12. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ... vivo and in vitro applications.   Figure 2: Modular features of DIRECTED particles where the ... more accessible by simplifying the production process and could even enable the creation of CAR-T cells...
  13. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...Nutr, 129 (2009a): 477–484. PubMed PMID: 10064313. 2. Chapman-Smith, A., & Cronan, J. E. “The enzymatic...These biotin dependent enzymes are essential for processes such as gluconeogenesis, lipogenesis, amino acid...energy transduction. Biotinylation is the general process by which biotin is added to a protein or macromolecule...biotinylation occurs naturally in cells and is the process by which biotin is covalently bonded to a specific...
  14. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...become a valuable asset yourself.     Fig 2: A diagram of the Thomas-Kilmann Conflict Model, courtesy...communicated. The next step is to come up with a viable process that will avoid such miscommunications in the future...and examples that have guided your resolution processes in the past. Types of Resolution There are of...
  15. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS) General protein expression...auxillary plasmids may be isolated during the prepping process. How does inducible expression work? As mentioned...
  16. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...Postdoctoral Associations around Boston - From Panel Session 2: Defining the Postdoc: An Institutional Perspective...questions or concerns throughout the publishing process. The diversity panel - Equity and inclusion The...
  17. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...signal separately for each target.   Figure 2: Examples of simultaneous multiplex IF labeling of... appropriate secondary antibodies. The general process is similar to standard immunofluorescence, but ...spatial analysis of cells in complex tissues. Proceedings of the National Academy of Sciences, 117(52),...
  18. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...of concept for further development. Figure 2: Mechanisms of deaminase-based adenine transversion...editor. Black arrows indicate natural cellular processes. Created with BioRender.com. The first deaminase-free...
Showing: 341 - 360 of 373 results