We narrowed to 695 results for: prot

...The catalytically dead Cas9 protein (dCas9) is well known for its ability to bind DNA targets without.... To do so, they fused a different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9...boxB) that each recruits a different fluorescent protein (BFP, GFP, or RFP) to the genomic loci of interest...) showing recruitment of different fluorescent proteins. (B) CRISPR-Sirius used to visualize relative ...Download our CRISPR eBook Learn about fluorescent protein-based tools Read all CRISPR blog posts Resources...

...structured surfaces. Plasmids expressing fluorescent proteins will be used to tag individual strains of bacteria...complications of diabetes mellitus. Recombinant human proteins that play a role in insulin regulation and diabetes...and the activity of the plant compounds on these proteins will be tested. Investigating changes in neuronal...condition is crucial. Plasmids expressing fluorescent proteins and a new two-photon laser scanning microscope...containing promoter regions that activate fluorescent protein transcription in response to river contaminants...

...CRISPR protein required is the Cas9 endonuclease or a variant thereof. This individual protein has all...RNA provided that target is upstream (5') of a protospacer adjacent motif (PAM) Cas9 endonuclease binding... RNA and a 3-base pair sequence known as the Protospacer Adjacent Motif or PAM. In order for dsDNA to ...HNH-like nuclease domain near the center of the protein. Upon target binding, Cas9 undergoes a conformational...

...binding with Rep proteins and mutations in the trs site reduce Rep nicking. Since Rep proteins are important...with the viral rep (replication) and cap (capsid) proteins, which bind to the ITRs and initiate replication...resolution site that serves as the target site for Rep proteins (Savvy et al. 2013). Figure 2: AAV2 wild type... based vector improvements unrelated to capsid protein modification. Front Med, 10, 1106085. https://doi.org...

...trying to find a relatively rare protein in an experiment with many proteins of interest! Research the brightness...  However, antibodies conjugated to horseradish protein (HRP), primarily used in Western blots and ELISAs...of the most complex subjects in antibody-based protocols - but thankfully this is an Antibody 101 post,...Try to inversely pair fluorophore strength with protein abundance.    Second, you’ll need to consider...

.... Antibody heavy chain proteins as well as the genes that encode those proteins are designated by the ...antibody isotypes. Binding to complement system proteins, which can initiate a cascade that helps attack... IgG2, IgG3, and IgG4. IgE IgE antibodies help protect against parasites but also play a role in allergies...secretions (e.g. tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly...

... GEMCherry2, an improved monomeric fluorescent protein for assessing CRISPR/Cas9 activity Guide RNA structure... labor-intensive, require multiple fluorescent proteins, and aren’t that efficient. To overcome these ...developed a modified monomeric red fluorescent protein (RFP), named GEMCherry2, that can rapidly and efficiently... M (2018) A Modified Monomeric Red Fluorescent Protein Reporter for Assessing CRISPR Activity. Frontiers... CRISPR blog posts Find more about fluorescent proteins Get a refresher on CRISPR with our CRISPR 101 ...

...Envelope proteins determine which types of cells a virus can infect. The VSV-G envelope protein enables... the Bob Weinberg lab and contains an envelope protein for producing lentiviral and MuLV retroviral particles...For more information, see the Addgene’s pLKO.1 protocol. Pro-tip: this plasmid grows more slowly than ...pSpCas9n(BB)-2A-GFP (PX461) expresses both a Cas9 protein and a gRNA. But instead of Cas9, this plasmid expresses...

...awards to early career scientists using fluorescent proteins in their research. These awards help early career...the development and dissemination of fluorescent protein tools. This year, we’ve made some changes. In addition...thiol-containing acceptors. These hydropersulfides modulate protein activity and have important roles during oxidative...inhibits the neuron (via hM4D). She fused fluorescent protein tags to the receptors to monitor their localization...-expressed with a photoconvertible fluorescent protein mEOS4a. Cells containing a sensor that shows a ...

... elements – promoters, origins of replication, protein tags, and antibiotic resistance markers (just to...as the “cyclization recombinase”), is a 38 kDa protein responsible for intra- and inter-molecular recombination... Cre acts independently of any other accessory proteins or co-factors, thus allowing for broad applications...single loxP site are recognized and bound by a Cre protein, forming a dimer. The two loxP sites then align... a parallel orientation, allowing the four Cre proteins to form a tetramer. A double-strand DNA break ...

...example: in a cell there are proteins which interact with other proteins and with DNA, and these complexes...multigene systems whose protein products can interact with the DNA that encodes the protein itself or other genes... genes. What if one of those genes coded for a protein that regulated its own transcription, or the transcription...

...detect, purify, quantify, deplete, and visualize proteins of interest (Greenfield, 2014). Traditionally,...chain for the antibody and when translated into protein will assemble into a fully functional antibody....encoded in a plasmid. (Right) An assembled antibody protein binds an antigen. Image from Fvasconcellos. ...genes for antibody expression. If starting from a protein sample (for instance, affinity purified antibodies...well in animals because of similarities to host proteins. After the antibody of interest has been cloned...

...Confirming tags and fusion proteins Finally, we confirm promoters, tags, fusion proteins, and selectable markers...activation screening. Nat Protoc 12:828–863 . https://doi.org/10.1038/nprot.2017.016...entry or using BLAST.   Confirming tags and fusion proteins. We’ll break down each of these steps below. ...

...bacteria CRISPR-Casɸ mGold, a new yellow fluorescent protein New CRISPR plasmids New items from the viral service...use light to induce conformational changes in a protein.    Cells expressing an RFP reporter plasmid...Cre, Opto-Cre-Vvd, by splitting and joining the protein regions with Vivid (Vvd) photodimers. Upon the ...mGold, the most photostable yellow fluorescent protein to date By Justin Ng High-throughput screening ...types expressing variants of Yellow Fluorescent Protein (YFP). The team identified a new YFP variant, mGold...

...incomplete. Protein expression strains are not good for cloning Bacterial strains adapted for protein purification...levels of protein, not plasmid DNA. If you need large amounts of DNA from a plasmid in a protein expression...a DNA prep kit or your lab's favorite in-house protocol. DNA yields for this procedure are typically in...

...DNA N-glycosylase (UNG) enzymes. Various other proteins included in different CGBE tools allow the researcher... human AAG (remember, MPG and AAG are the same protein!) (Chen et al., 2024). They called their initial...considerably. And there’s so much more exciting protein engineering going on in the base editing field!...hypoxanthine from DNA, creating an abasic site. The same protein (or its homologs) has more than one name. Indel...Huang, Q., Ma, Y., Yuan, F., & Chang, X. (2024). Protein language models-assisted optimization of a uracil-N-glycosylase...

...that affected gene expression, mRNA splicing, or protein coding regions. In total, they tested 13 strains...phenotype, but only one, Ly6a, showed differential protein and RNA expression between the brains of C57BL/...similar to the Deverman Lab when they looked at LY6A protein and RNA expression patterns, the PHP.B permissive...17379763. Hordeaux, Juliette, et al. "The GPI-Linked Protein LY6A Drives AAV-PHP. B Transport across the Blood-Brain...Find ready-to-use AAV from Addgene or check out protocols for packaging your own virus ...

...based on what happens to an organism when a gene/protein is depleted in a knockout experiment. Certain scientific... break room for Addgenies, was named after the protein Groucho, a transcriptional co-repressor important...important in development. Groucho family proteins are found in flies, worms, frogs, mice, and even humans. ...Nusslein-Volhard and Wiechaus, 1980). The mammalian hedgehog protein, sonic hedgehog (SHH) was named by a postdoc in...including oogenesis and cytokinesis. Why name this protein after a vegetable substitute for spaghetti? I actually...

... vague and "what proteins regulate the Z pathway?" is an open-ended list of proteins. Instead, consider...of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent proteins,  and live cell...consider "how do proteins X and Y regulate the Z pathway?" A well-defined outcome makes it easier for the search...

...generally validate our viral vector preparation protocol, and we are pleased to report that over 95% of...Primer annealing can be affected by the presence of protein contaminants, which is a factor in AAV titration...viral vector solution (which contains viral capsid proteins) as opposed to purified DNA. This may be mitigated...preps contain similar and low levels of background protein and chemical components, ddPCR may not be an improvement...More about Virus Production Find Viral Vector Protocols ...