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Showing: 341 - 360 of 597 results

  1. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...encoded tag and exogenously added components. For a review see Lotze et al. (2016). To date, small probes ... tools engineered from phytochromes." Chemical reviews 117.9 (2017): 6423-6446. PubMed PMID: 28401765....

  2. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...antibody dilution — both factors we’ve covered in our previous blog posts (1, 2) on western blotting. If the ...confidence in the accuracy of western blots. Expert Review of Proteomics, 11(5), 549–560. https://doi.org/...

  3. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...co-opted toward that aim (i.e. sacB). Importantly, to prevent activation of toxins before the second crossover... in 5 days. Counter-selection with sacB has revolutionized allelic exchange. Even now, only half our lab...

  4. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...expensive sequencing fuels a sequencing revolution that still prevails. Sequencing whole genomes or profiling...

  5. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...expressed for a short period of time, which would prevent the accumulation of unwanted mutations over time...upregulating HDR, NHEJ must be downregulated to prevent the introduction of new mutations by CRISPR; editing...

  6. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...your success. Therefore, it only makes sense to reverse engineer your budgetary needs from your experimental...going through some of the questions we mentioned previously.  There is value in the process of creating a...

  7. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...an alternative but well-studied kinase known to prevent tau hyperphosphorylation. You could run brain samples...means an exhaustive list and we encourage you to review the literature for your specific experimental technique...

  8. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    .... Of note, most of these optimized Cas enzymes prevent cutting at mismatched guide sites, but not necessarily...alternative pathways. See (Guo et al, 2023) for a review of other targeted sequencing methods. Candidate...

  9. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...retrotransposon insertion, a combined endonuclease-reverse transcriptase uses homology arms flanking an RNA...repair (HDR), and insertion is scarless, unlike previous integrase-based approaches. Find STITCHR plasmids...

  11. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ... anneal to the backbone in either a forward or reverse orientation so you’ll need some way to verify that...digestion, you will need to use a phosphatase to prevent re-circularization of the backbone plasmid (see...

  12. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...super-resolution imaging of ER and mitochondria reveals dynamic organelle interactions. a) Labeling scheme...filament assembly-regulating proteins in vitro reveals complex events. a) Micrographs of a fluorescently-labeled...

  13. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...really does not take much time at all. Much of the previous literature using AAVs for DREADD delivery indicates... of neurons showing a very specific phenotype. Previous neural tracing methods used viruses that were ...

  15. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ... should review before using an antibody.  Antibodies and Reproducibility First, let’s review why validation...

  16. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...excitation. After a short period, the electrons revert to a lower energy state, whereby a photon is emitted...markers they label) can occur. As I mentioned previously, you can also use fluorescent reporters, such...

  17. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...the original reference library, as well as GC%, reverse compliment sequences, and more. Output_Compact:...Wang, Tim, et al. "Gene essentiality profiling reveals gene networks and synthetic lethal interactions...

  18. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...Single Molecule Localization (PALM/STORM), or Reversible Saturable Optical Linear Fluorescence Transitions.... Lens-based fluorescence nanoscopy. Quarterly reviews of biophysics 48, 178-243 (2015). PubMed PMID: ...

  19. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...preparation, staining procedures, and controls. To prevent instrument clogs, ensure that samples are single... LA (1972) Fluorescence Activated Cell Sorting. Review of Scientific Instruments 43:404–409 . https://...

  20. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...evolutionary classification of CRISPR–Cas systems. Nature Reviews Microbiology, 13(11), 722–736. https://doi.org/... burst of class 2 and derived variants. Nature Reviews Microbiology, 18(2), 67–83. https://doi.org/10.1038...
Showing: 341 - 360 of 597 results