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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 640 results for: son

Showing: 341 - 360 of 640 results

  1. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ... A., Sousa, A. A., Tsai, S. Q., Bengtsson, N. E., Lööv, C., Ingelsson, M., Chamberlain, J. S., Corey, ...

  2. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...Byron Rubin, a crystallographer working with Jane Richardson, provided an initial solution to this problem...are interested in experiencing Cas9 up close and personal, visit the 3D Molecular Designs web site. Or, ...

  4. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...to capitalize on your experience. No matter the reason for attending, your preparation and day-of game...hear or understand what we are being asked. I am personally guilty of this, and have many times answered ...

  6. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...Thankfully, this is a hot topic with many resources! In Bondeson et al, they review all the tags discussed here...informed choice! References and Resources References Bondeson, D.P., Mullin-Bernstein, Z., Oliver, S. et al....

  7. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...these therapeutics. Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid...identified.     New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

  8. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...resources References Pyhtila, B., Kasowitz, S., Leeson, R., & Barrangou, R. (2023). The Expanding Dissemination...  Rachel Leeson...

  9. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...Why do these organisms have chromoproteins? One reason may be that chromoproteins protect corals from ...fluorescence lifetime imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) in living cells (Figure...

  10. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...consider performing PEG precipitation. Instead of sonicating the cell pellet, I lyse the cells with this AAV... Stone and Harshana de Silva Feelixge at Fred Hutchinson Cancer Center for teaching me about AAV production...

  11. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...science and society." Science 376, (2022): 250-252. Carlson J, Henn BM, Al-Hindi DR, Ramachandran S. Counter...  Rachel Leeson...

  12. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ... figure by figure organization with a different person responsible for each figure. Others still are more... avenues including casual request via email, in person, or instant messaging but we as scientists often...

  13. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...why should cell biologists talk to chemists? One reason is something called “the photon budget”: each sample...Protein Fusions Request Plasmids from the Michael Davidson Collection Find Fluorescent Protein Kits ...

  15. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...philosophy of  “work hard, play hard.” Part of the reason we sincerely like spending time together is that...work” all the time. She was a wise lady for many reasons, but this saying is especially true. If your event...

  16. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...Pk1] in a western blot assay. Image reused from Anderson et al., 2024 under CC BY license.    Find ...Find Anti-V5 [IPI-SV5-Pk1] Antibody here!   Anderson, Z., Zhu, H., Riedel, T., Moshinsky, D., Meijers, R...

  17. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...regulatory elements or use a non-canonical code (Gustafsson et al., 2004), such as Hoogsten base pairs and...Biol 13:30–34 . https://doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias...

  18. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...Langan, E., Siebert, S., Li, J., Greiner, D., & Anderson, D. (2015). Neutrophil Responses to Sterile Implant...  Rachel Leeson...

  19. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...References Lock, M., Alvira, M. R., Chen, S.-J., & Wilson, J. M. (2014). Absolute determination of single-stranded...https://doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., & Feschenko, M. (2018). Determination of ...

  20. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...proteins, and modifications to the pegRNA. The Britt Adamson Lab recently used a CRISPRi screen to identify ...useful materials for research into Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease, including plasmids...
Showing: 341 - 360 of 640 results