... a DOI referencing primary literature, and every effort is made to moderate and cross-check all data with...affected by environmental conditions (such as temperature, pH, fusion protein, etc...).  There are many... Addgene blog – outlining various important considerations when choosing a FP, but much of the primary... making such a decision remains spread across literature in publications that introduce these tools. ..., and I collaborated with him on a web-based interactive visualization of FP attributes (which turned ...that allows users to see when a given page has unmoderated changes and to view previous versions.  Currently... with published literature as soon as possible after submission. Using FPbase The most obvious use case...

...your incubation temperature. Some proteins aggregate at 95 °C, a common lysis temperature, so consider a...background noise. Temperature and time You may need to optimize your antibody incubation temperature and time.... variants Check literature Posttranslational modifications Check literature Highly charged...said, normalizing to total protein expression is preferable, and more accurate, than normalizing to ubiquitously...recombinant multiclonal antibodies to increase overall signal from the primary antibody.  Pro tip! If ...common conditions are 4 °C overnight or room temperature for 1–2 hours, there is some evidence that antibodies... including trying longer incubations at both temperatures if needed. Washes Check your antibody manufacturer...

...biotinylation, the discovery of the biotin-avidin interaction and the uses of biotinylation in molecular biology... and energy transduction. Biotinylation is the general process by which biotin is added to a protein or...protein (BCCP). Discovery of the biotin-avidin interaction   Figure 1: Biotin is enriched in egg ... of the strongest noncovalent protein-ligand interactions found in nature. Once the bond is formed it ...it is unaffected by changes in pH, temperature or organic solvents. Uses of biotin in molecular biology...scientists began to exploit biotin and its strong interaction with avidin for molecular biology and biotechnology...that are coated with avidin. The biotin-avidin interaction has also been adapted for use in immunohistochemistry...

...we’ll go through some of the most important considerations to make when planning and performing an IF ...IF experiment. What is immunofluorescence? Generally speaking, IF is an application in which antibodies...iBiology). IHC and ICC are essentially just more general terms that can also refer to assays using non-fluorescent... visualize their target. These antibodies are generated against a specific antigen and then conjugated... more common is that secondary antibodies are generally polyclonal, which means that multiple secondary...amplification. Another reason is cost effectiveness. Generating and validating primary antibodies is a time consuming...antibodies that exist and you can begin to see why generating fluorophore-conjugated versions for each primary...

...many distinct CRISPR therapies to match these different mutations. For each therapy, effectiveness and ...’s size makes it difficult to deliver via gene therapy, so researchers have set their sights on another...work with dystrophin exon skipping? For such a therapy to be successful, multiple requirements must be...minimal risk of off-target editing. In order for the therapy to persist over time, stem cell editing would be...in DMD, and it’s estimated that exon skipping therapies would be applicable to 80% of DMD patients. ...characterizes additional, unique facets of CRISPR DMD therapy. Long et al. show that AAV-CRISPR does not cause...represent a great step forward for CRISPR gene therapy, it’s important to realize that DMD is a simpler...

...induced binding. The lab used this general design scheme to generate two more OptoNBs, one against mCherry...loop 1 always generated OptoNBs with dark-induced binding, but insertion into loop 6 generated both blue ...purification. After binding YFP to the column, you wash several times to remove non-specific proteins, and then...on the key components of OptoNBs, how they are generated, and their use for protein purification and controlling...chain antibody domain.  How do these components interact? When fused together to form an OptoNB, the AsLOV2...nanobody’s ability to bind its target protein.  Generating OptoNBs The opening and closing of the AsLOV2...over-activated in cancer due to its involved in cell proliferation and differentiation.  Since Ras is a membrane...

...Brookline Interactive. Learn more about Carl Paratore, MBA, CPA Mr. Paratore serves as the General Auditor...Suffolk University Law School and Paratore is the General Auditor at Tufts Health Plan. The addition of Katz...perspective, what do you think are important considerations for the future of open science? What about ...University Law School. Suffolk University graduates on average 20 PhD’s a year and we have a very dominant presence...presence in the biotech sector in both patent law and general life science regulatory law. My role at Suffolk...work at Tufts Health Plan and my role there is general auditor. I oversee a department of 20 individuals...the efficiency and effectiveness of the Plan's operations. We’re looking for those “what could go wrong...

... used to generate cDNA, which  was used as a template for PCR amplification with a degenerate primer set...limitation of hybridoma generated mAbs is that if the frozen cell line becomes non-recoverable, the stock of the... distributed by Addgene.  The plasmids used to generate these antibodies were based off of a large collection... they were also able to isolate RNA from non-recoverable hybridoma stocks, without having to grow up the... the cells. This RNA could then be used to generate functional recombinant monoclonal antibody (R-mAb)...manner. Cells were labeled with both a hybridoma generated mAb and the R-mAb, with the R-mAb being verified...encoding sequence within the plasmid backbone to generate R-mAbs of different subclasses, including subclasses...

...made much easier if the interaction between agent and customer is cooperative rather than combatitive.... daydreamed about the ideal customer service interaction - one that gives our customers the most utility...apply to any biology-related customer support interaction. Before contacting customer service In an age...that for you” meme is infamous enough to have generated it’s own tongue-in-cheek website.  If you are ...catalog number ready. I've attempted to answer several emails that start: “Hi! We ordered something from...concern. Please be courteous. We understand how exasperating it can be to lose time and money on a potentially...re going to get. There are questions that are answerable in minutes and questions that could take weeks...

...virus. In general, viral envelopes dictate infection of target cells through their interactions with target... between neurons are what define how the brain operates, and thus, are a major part of the answer to many...individual neurons they infect. However, they generally are not transmitted to adjacent neurons via synapses...monosynaptic tracing was used to examine how adult-generated neurons integrated into the brain over time (Deshpande...This study addressed the question of how newly generated neurons integrate into the complex pre-existing...the functions of these two neuronal populations. Overall, this study laid the foundation for how different...the brain receive inputs and compute outputs. In general, RABV monosynaptic tracing is a powerful tool that...