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We narrowed to 729 results for: NSI

Showing: 181 - 190 of 729 results

  1. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...are some potential complications that must be considered when using this method:     Cells must be amenable...to directly deliver a ribonucleoprotein (RNP), consisting of the Cas9 protein in complex with a targeting...gRNA from a commercial vendor, this can often be expensive. An alternative approach is to express and purify...is most appropriate for a given experiment. Considerations for RNP-mediated CRISPR experiments While using...with these types of experiments. One important consideration is that the Cas9-gRNA RNP will be degraded by...

  2. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...immunoprecipitation (locus-specific ChIP). These methods consist of insertional chromatin immunoprecipitation (iChIP...Advisory Board.  Addgene: Your lab has worked extensively with enChIP systems. Can you describe this technology...technologies. The locus-specific ChIP technologies consisting of iChIP and enChIP are recent examples. We also...community is a kind of duty for researchers when considering the peer-review system of journals and grant ...Identification of Proteins Associated with an IFNgamma-Responsive Promoter by a Retroviral Expression System for...

  3. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...and it will teach them to respect their own responsibilities. Another tip: don’t miss your grandfather’... the evening, or in a few years) when life responsibilities are less You can work at home sometimes, but... the burden alone You, alone, are not solely responsible for care and attention to your home and family...doctor without your help. Your partner is equally responsible and capable. The only exception is that men cannot...tasks – try to resist the impulse to control by insisting things always be done your way. Be patient with...

  4. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ... package stays in transit for a certain number of days and tracking average transit time for each courier...having some materials available to order, yet not consistently accessible, wasn’t an option. So we identified...For example, when we began offering temperature-sensitive materials, we noticed a large percentage of these...distributor allowed us to ensure that temperature-sensitive materials like viral preps and libraries were ...

  5. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...Specificity concerns? If off-target effects are a worry, consider a dual-sgRNA system. This system uses a “nicking...little homology to other regions of the genome and consider using a Cas9 variant with higher specificity (...requirements, giving it more flexibility. A consideration with this tool is that the desired edit will...activators The simplest of the dCas9 activators consists of dCas9 fused to a single transcriptional activation...Mediator complex, generally the strongest activator, consists of a dCas9-VP64 fusion and a modified gRNA that...

  6. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...viral vector solution. Since this technique is intensive and requires an electron microscope, it is not...image shows that the vast majority of the vectors consist of full particles (white arrowhead) relative to...from different sources in the same experiment, consider retitering both AAV in your lab. The absolute ...does not give an indication of infectious titer, consider titrating each lot of AAV in vivo to determine...Additional Resources on the Addgene Blog Important Considerations When Using AAVs Addgene's AAV Quality Control...

  7. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...resistant to antibacterial drugs. Resistance can be intrinsic, meaning a basic characteristic that naturally...resistant somehow overtime. One fantastic example of intrinsic resistance would be Mycoplasma species’ resistance...possess cell walls. As a result, Mycoplasma are intrinsically resistant to this class of antibiotic. Acquired...be unharmed by the compound it produces (think intrinsic resistance), but surrounding microbes would be...prescribed to them This may seem counter-intuitive considering the other three scenarios, but a short treatment...

  8. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...emerged from intensive engagement with a wide range of people at the University of Wisconsin in 2011/12 ...reproducibility is limited in two ways – only data inside the ELN is reproducible, and only the highly restricted...principles emerging from the engagement with the Wisconsin research community were to: (1) prevent vendor... choosing an ELN for your research, be sure to consider how its features do and don’t support reproducibility...

  9. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...AAV delivered genes. AAV serotypes have been extensively studied in order to define those that target ...weakly expressed. As these epitopes tags have no intrinsic visual signal, they cannot be detected directly...neurons. As compared to epitopes tags, FPs are intrinsically fluorescent and can therefore be used in live...darkened chromophores. These probes are both intrinsically fluorescent and can be detected with highly ...tags like GFP. Specifically for STORM, the high intensity signal provided by smFPs can result in better ...

  10. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...sure your cells have first reached an appropriate density. Cells in the exponential growth phase are alive... prevent the culture from reaching the desired density within a reasonable time-frame. For these cases... growth media, or if your plasmid is low-copy, consider reducing the antibiotic concentration. Growth ...accomplished by growing your culture to the desired density at 37°C and reducing the temperature or moving ...TB or 2XYT is optimal because of the high cell-density they support; however, minimal media supplemented...
Showing: 181 - 190 of 729 results