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We narrowed to 630 results for: Son

Showing: 181 - 190 of 630 results

  1. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...visualization tools using CRISPR In 2015, Thoru Pederson’s lab at the University of Massachusetts Medical...CRISPR-Sirius Recently, Hanhui Ma and the Thoru Pederson lab developed CRISPR-Sirius (Ma et al., 2018)....order to label two loci simultaneously, Ma and Pederson created another sgRNA containing the PP7 hairpin...

  2. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...science trainee will spend 4-7 years working for one person and with a group who all strive for a common big-picture...rotation, which reveals their working habits, personality, and gives them the opportunity to see if the...extreme openness” of the lab (i.e projects like the Personal Genome Project and open source software, hardware...

  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...comparable experimental results. Using PiggyBac transposons, the Woltjen Lab compared different variants ...reprogramming discrepancies. The polycistronic piggyBac transposon vectors tested in this work are available at ...tool for use as a donor in FRET (fluorescence resonance energy transfer) and FLIM (Fluorescence Lifetime...1kb fragments are produced (see figure above). 3 Reasons The PSU Ladders Are Awesome Tools: They are comprised...

  4. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...error rate for long sequence knock-ins by: Lucie Wilson Le Cong’s lab has developed a method using dCas9...error rates overall ranged from 0.3% to 10%, in comparison to the 62-90% error rate seen with Cas9 editors...barcodes for live cell clonal multiplexing by: Rachel Leeson Multiplexed images in live cells remains a challenge...

  5. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ... variations in DR sequences. Figure 2: comparison of Cas 9 vs. Cas 12a expression cassettes when...0600-6. The Penn State Protein Ladder  by: Rachel Leeson This simple, inexpensive plasmid-based ladder system...mammalian genome regulation and editing by: Rachel Leeson The large size of CRISPR-Cas effectors and fusion...

  6. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B, Aldred SF, ...JoVE. https://doi.org/10.3791/54832  Lipman NS, Jackson LR, Trudel LJ, Weis-Garcia F (2005) Monoclonal ...

  7. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ... Cpf1 for multiplex genome editing.  For a few reasons, Cpf1 is a simplified system for editing multiple...guide page for a review of Cas9.  For a brief comparison of Cpf1 vs. Cas9, see the table below. Table...expression vector.  See the graph in figure 2 for a comparison of editing frequency resulting from transfection...

  8. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ... proto-oncogenes and disrupt their expression (Robinson, 1982). To add insult to injury, retroviruses ...immunogenicity low! References and Resources References Robinson HL. Retroviruses and cancer. Rev Infect Dis. 1982...Gene and Cell-Based Therapies in the New Era of Personalized Medicine. Genes Dis. 2017 Jun;4(2):43-63. DOI...

  9. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    Published
    July 28, 2015, 2:30 p.m.
    ... the genome. Mendenhall and Richard Myers of HudsonAlpha (where Mendenhall is also an adjunct faculty ...the function of the protein. Myers: We’ve done comparisons of our new system versus standard ChIP-seq for... for transcription factors, but there are many reasons you might want a system like this. It’s actually...

  10. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ... Renaud J-B, Sallmyr A, Ruis B, Oh S, Tomkinson AE, Hendrickson EA, Giovannangeli C, Jasin M, Brunet E...P, Burkhalter MD, Oh S, Qaqish BF, Moore DT, Hendrickson EA, Ramsden DA (2014) The fidelity of the ligation...
Showing: 181 - 190 of 630 results