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We narrowed to 847 results for: STI

Showing: 361 - 380 of 847 results

  1. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...2016 Addgene’s research team has been actively investigating ways to improve viral vector production. Whether...Whether redesigning viral packaging plasmids, investigating different viral production strategies or developing...were unable to deduce the mechanism responsible we still believe that these results will be beneficial to...method to improve AAV yields. Although we only investigated 3 serotypes it’s possible that this approach...

  2. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ... have the potential to provide improvements to existing technologies based on polymerase processivity....stable, highly soluble, and non-immunogenic yet are still just as specific to their target as regular antibodies...podcast segment Steven Carr’s lab at the Broad Institute focuses on proteomics: developing and applying...analysed. In this work, the authors specifically investigated protein binding at the TERT gene, but this method...

  3. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ... mountain – with many routes up and only one destination – a career in the sciences is more like an intergalactic...developmental programs unfold, absorbing the stochasticity of molecular interactions and embedding individual... weird and rare organisms so I found it hard to stick to vertebrates alone.  And a longstanding inclination...with things like word choice, punctuation, and stylistic consistency. If you’ve ever doubted the importance...

  4. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ... replicate the host's chromosomal DNA, but they still rely on the host machinery to make additional copies...Patrick says researchers can ask themselves a few questions to get started: Will the plasmid be used exclusively...that plasmids with low to medium copy numbers can still express massive amounts of protein given the proper...cells have not started to die off. Have any questions? Need something clarified? If so please, post ...

  5. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ... of the primer assist with restriction enzyme digestion (usually 3-6bp) Restriction Site: Your chosen...and then TAAGCA to improve restriction enzyme digestion. This gives us a sequence of 5'-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA... You can generate the reverse-complement using existing software (a quick internet search will lead you... The PCR product is now ready for restriction digestion. As such, the later steps in this process are ...

  6. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...labs. We’re overjoyed to provide you with these fantastic educational resources and will be sure to keep...us at [email protected]. Thank you again for a fantastic year and happy reading! Top 10 blog posts of 2015...UNC Chapel Hill explain some of the molecular gymnastics behind DNA repair by non-homologous end joining...career coaches do and offers up some career coach suggestions. 8. A Match Made in Heaven: CRISPR/Cas9 and ...

  7. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...Serendipitous Stain. https://hardydiagnostics.com/wp-content/uploads/2016/05/Hans-Christian-Gram.pdf Smith, A. ...infected lung tissue, a Danish biologist named Christian Gram realized that certain stains were retained...Stain. https://my.clevelandclinic.org/health/diagnostics/22612-gram-stain Hardy, J. Gram’s Serendipitous...

  8. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...scientific question that they are eager to discuss with others and sending out these questions ahead of ...Figure 1: Virtual lab meeting? No problem! You can still creatively collaborate and brainstorm! Image from... be written by several authors from different institutes, disciplines, and cultural backgrounds.” This...

  9. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...for sleep. At Addgene, our mindfulness group is sticking with their Headspace practice even while working...for bench scientists to assist in labs that are testing for COVID-19. You can also give back by supporting...interview with you, or look for professional societies hosting virtual networking events. Download Addgene’s Science...expectations for your productivity Set a routine and stick to it. Plan defined work hours as well as time for...

  10. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    Published
    Dec. 10, 2019, 1:58 p.m.
    ... T helper type 2 (Th2) cells from mice that constitutively express Cas9 and screen for genes that affect... Steven Farber and colleagues at the Carnegie Institute for Science have developed the LipoGlo system ...UAS). The Wang Lab created a tripartite system consisting of dCas9, a gRNA fused to an RNA binding motif...displays high dynamic range. However, the promoter in question must be σ54-dependent and the system may not be...

  11. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...complicated entities with a large range of characteristics (color, brightness, photostability, maturation...can submit new fluorescent proteins, add data to existing proteins when missing, or edit inaccuracies as...these parameters and you’ll find matching FP suggestions. If you’re not sure exactly what you’re looking...Plans for future development of FPbase FPbase is still very much in the early stages of development, and...

  12. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...and white. The decor was very contemporary and futuristic with block chairs in the hallway for people to...what to expect at a workshop and what kinds of questions to ask myself when I am bombarded with a lot of...’m hoping to learn from this workshop? These questions will help me avoid being overwhelmed by all the...instead allow me to pay attention and answer my key questions. As an added bonus, I also got the opportunity...

  13. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...Relationships need nurturing. Perhaps you’ve met someone interesting at an event. Or, perhaps you have a colleague... “it was nice meeting you.” Include follow-up questions or requests for advice based on your discussion...LinkedIn I once helped by answering one simple question so I accepted the connection invitation. They ...became closer. She supported me and answered my questions and honestly, she was the only person who really...

  14. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...just focus on the parts where people had questions or suggestions, instead of reading the whole thing together...gets a chance to talk and less vocal team members still have their voices heard. Here's a template for team...other’s projects. For all-company meetings, monthly still works best. Figure out what works for you for each...

  15. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...experiment.  For example, I used to constantly underestimate time needed to prep for an experiment (labeling...protocol, from a full page to a few key reminders on a sticky note   Visual Planning  Plate maps, pre-labeling...you need to be successful! Slowing Down At the suggestion of a postdoc, to whom I am forever grateful, ... of things, including (and maybe especially) suggestions you are initially resistant to. Just like any...

  16. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...answer to any of these questions is “yes,” then I would suggest either testing the antibody efficacy through...incident to your records, and consider the following questions as you move forward:  Was a light-sensitive conjugate...for your ELISA and Western, I’d highly recommend sticking with what works.  To balance this out, however...

  17. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...biology – they can deliver materials to specific destinations, haul a variety of cargo, and even handle a ...cassettes typically contain a promoter (inducible or constitutive), your gene of interest, and often a selectable...your construct in target cells transiently or constitutively depending on the virus used. All of these ...response 2) very high immunogenicity 3) and long-lasting immune response even after a single dose. ...

  18. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...a talk about the different parts of the brain stimulated by stories. In retrospect, I should have expected...neuroscience and you don’t have a background in statistics and programming, you’d be wise to start working...surprised at how easily I could jump in and ask questions about the various posters I visited. If you’re...neuroscientist looking to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene...

  19. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...focus of the application was changed, but Sample6 still leverages the core engineering and scientific concepts...communication styles in academia and industry vary drastically, he reassures that one can learn it by reading...into the local academic community. However, Mike still could not remove himself from the idea of starting... many universities throw out old equipment that still works. “We use functional spectrometers from the...

  20. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...Mentoring series will cover 2 of the 7 mentoring questions I set out to answer. First, I will describe some...diligent about replying and thinking about good questions and answers. Professional networking groups will...and move on. If they say yes, be prepared with questions and topics to discuss. The mentee is responsible... needs to be open to change and constructive suggestions and feedback. The best thing to do to make your...
Showing: 361 - 380 of 847 results