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We narrowed to 885 results for: ANT;

Showing: 541 - 570 of 885 results

  1. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...traveling for campus visits. This is definitely an advantage of surviving in a virtual world over the course...Having notes close to your computer with things you want to highlight in the interview is another way to ...staff have become a new way to allow you to get important information about the campus even if you can’t...and the in-person portion got canceled, taking advantage of virtual meetings can still be a great opportunity...exception. However, using the virtual world to your advantage can strengthen your application and help you decide...

  2. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ... in high-throughput libraries My labmates and I wanted to find a way to predict the efficiencies of pegRNA...Figure 1). Through this method, we found key determinants of prime editing efficiency such as the length...et al., 2024) have highlighted how chromatin substantially affects prime editing outcomes across many genomic..., A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante, F. G., & Parts, L. (2023). Prediction of prime...efficiencies using sequence features and DNA repair determinants. Nature Biotechnology 2023, 1–11. https://doi.org...

  3. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...gRNAS1, and pAdSh.U6.gRNAGFP. Gonçalves says that advantages of AdVs include their episomal nature and very...efficient introduction of DNA into therapeutically relevant, non-transformed mammalian cells. These viral ...Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used so far) or for cancer...used, the CRISPR approach based on HDR has the advantage of being a potentially universal treatment (Stephens... could be realized via simple switching of the relevant gRNAs and donor DNA Adenoviral CRISPR/Cas9 genome...

  4. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ... your research. RNA editing advantages RNA editing has multiple advantages over more traditional DNA editing...stretches of duplexed RNA. Using an ADAR2DD catalytic mutant, they showed that editing is mediated by ADAR2DD...precise than shRNA knockdown, it still displays substantial off-target activity. By mutating residues in ...discussed above, the ability to edit RNA has multiple advantages, including reversibility and use in non-dividing...

  5. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...months, pausing or terminating employment for a significant fraction of the workforce. Wet lab scientists... open-ended question is difficult to answer quantitatively without broad, unbiased data. Therefore, we...cases being reported (right, red), likely due to quarantining {% video_player "embed_player" overrideable...exponential decay model, with dips during weekends and significant holidays. Figure 4: Plasmid request trajectories...parameters from relatively sparse data from China. An important additional caveat is that our dataset contains...

  6. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...and reported results are encouraging. Importantly, a significant amount of basic research is being done...that can be monitored non-invasively over time. Importantly, it is an immune privileged compartment, meaning...meaning that it can tolerate the presence of antigens (such as viral vectors) without triggering a destructive...improve the technology: engineering of new capsid variants, designing expression cassettes to regulate and...

  7. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...learning experiences happened in the lab, and he wanted to provide the same kinds of opportunities for ...interaction with other proteins.   It is important to note that research activities in a CURE must...hypotheses being rejected, etc.), while reminding participants of the value of this approach and encouraging...hMDH1). They then expressed the original and recombinant enzymes in bacteria, purified them to near homogeneity...by making a greater number of students active participants in the research process – so if this sounds ...

  8. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...below). Let’s take a look at the features and advantages of the optimized human CRISPR libraries. Brunello... enough for efficient library performance, an important criterion for probing a limited number of cells..., confirming again that sgRNA design plays a significant role in library efficiency. Try the Dolcetto ...al. demonstrated that the Calabrese library substantially identified more hits than the SAM library approach...Libraries available at Addgene Learn about the advantages of pooled CRISPR libraries for large-scale functional...

  9. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ... should not even be the real goal. We are all constantly negotiating our energy and attention. The goal...you’ll need to care for an elderly family member, want to train for a triathlon, finally buy that charming... someone there clapping more often than not. You want your family and friends to know you care, but they...they need to know that you have an important life too. They will respect you if you show respect for your...You can handle that, right? Big tip: this is an important topic to discuss and agree on before you get enmeshed...

  10. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...detectable non-specific activity. Its major advantage is the significantly reduced amount of off-target toxicity...enzyme can lead to off targets and result in significant, negative effects in RNA targeting experiments...using Prime editing alongside some creative (and significant!) protein engineering. They combined Prime editing...The potential of CRISPR has yet to fully taken advantage of, but exciting new tools like these represent...

  11. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ...were less significant than when non-targeting guides are used as controls. Less significant p-values lead...genome-wide screens.   Figure 1: Summary of the advantages of the new approach to genome-wide CRISPR screen... Screens Before diving into the results, it’s important to note that in Morgens et al., guides were considered...of on-target activity. However, there was no significant difference in depletion of ricin sensitizer genes...

  12. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...External collaborations are becoming increasingly important as more complex approaches to scientific questions...collaborations and partnerships are also very important in a non-research context, especially when considering...initiative to find a suitable partner, connect the relevant teams, coordinate implementation, and now must...start and maintain external collaborations is important? In my current job at Addgene I work with people...work with others outside your organization is important for success in research-intensive careers. In ...

  13. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...viral genome and the absence of unexpected DNA contaminants. The data is also used to determine recombination...properties of the final vector preparation, the quantity of gram-negative bacterial endotoxin is ensured...within representative vector preparations was quantified with electron microscopy after negative staining...Approximately two weeks post-thaw, cell culture supernatant is tested for mycoplasma contamination. To date... Addgene's Data Hub Digital Droplet for AAV Quantification Viral Vectors 101: An Introduction to AAV References...

  14. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...thought to activate programmed cell death or a dormant state for bacterial cells that have been infected... Cas13a orthologs can function in mammalian and plant cells (Abudayyeh et al., 2017). They characterized...displays similar knockdown efficiency but no significant off-target effects, a distinct contrast to the... In short, the Cas13a molecule brings a much anticipated element of diversity to the CRISPR toolbox and...Check Out Our CRISPR Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 Resources...

  15. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ... comments on the AMA process below, but we also wanted to share some thoughts on why we decided to do ...Software for Biology) on Reddit and got some substantial traffic that we thought it would be a good idea...worried about selling yourself and really do just want to get information about yourself and your work ...internet content for scientists in training is abundant, helpful, and sometimes excellent. Finally, as...as I said in the AMA, it’s incredibly important to find good mentors and incentivize good mentorship: ...

  16. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...volume of lower titer virus. In such cases you may want to consider concentrating your viral prep. Storage...al. found that lentivirus produced at pH 6 are resistant to multiple freeze thaw cycles. Consequently, ...protein attaches to cell surface receptors, other important binding events take place during the course of...source. Labs that routinely produce lentivirus may want to consider testing a variety of cation and serum...Ward T.H., Margison G.P. “Mycoplasma infection significantly alters microarray gene expression profiles.”...

  17. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...thanks to our guest bloggers Shravanti Surest from Iowa State University. Shravanti Suresh is a PhD candidate...This post was contributed by Shravanti Suresh from Iowa State University. Since its appearance, SARS-...cut will continue to flow and be captured by an antibody further along on the strip. Highlights: Of the... cDNA. Isothermal amplification is done at one constant temperature, eliminating the need for thermocyclers...

  18. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...should be supplied (Baloban et al., 2017).  It is important to consider effective brightness of NIR FPs in...molecular brightness (a product of the fluorescence quantum yield and the extinction coefficient) (Shcherbakova...They were engineered to incorporate BV. Their advantages are their small size (17 kDa for miRFPnanos, ... highlighted in blue. aExtinction coefficient. bQuantum yield. cDetermined in mammalian cells. dDetermined... near-infrared proteins provide a scaffold for antigen-dependent fluorescent nanobodies. Nat Methods submitted...

  19. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ... out of fashion but is still useful and most importantly, effective. In the research world, there is a...plasmids The design of shRNA plasmids requires three important steps: Designing the shRNA sequence Choosing ...polymerase II promoters. The design of these plasmids is meant to more closely mimic pre-miRNAs, and the use of...19px;font-family: helvetica;","color":"#FFFFFF","variant":null,"font":"helvetica","style":"font-family: ...resources on the Addgene blog RNA Interference in Plant Biology: New Tools for an Old Favorite CRISPR 101...

  20. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...but it’s also important not to load yourself up with too many tasks. If you’re constantly switching between... recommend the book, “Bored and Brilliant” for learning more fantastic reasons to remove yourself from...ll discuss in the planning section below, it’s important to dedicate specific amounts of time to specific...

  21. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...good article at NatureJobs: Interviews: The All-Important Job Talk. 1. Watch other people give great talks...Think Keystone Keynote presentation or Ted Talk. You want to give the audience something to think about, argue... time to prepare (and it does take time). For important talks, ask your peer mentors to check your slides...Speaking longer just makes you seem unprepared or arrogant. A bit short is always better than too long, and...to practice and over time you can master this important science skill. Additional Resources Joanne'...

  22. Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!

    Type
    Blog Post
    Published
    Dec. 11, 2017, 2:02 p.m.
    ...CRISPR tools for use in plants.  Listen to the segment on CRISPR tools for plants. Special thanks to our...enhancing iPSC production, and CRISPR tools for plants. You can find additional hot plasmids in our quarterly...: Interview with Darrell Kotton Find additional plant biology blog posts Resources at Addgene.org Sign...

  23. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...CRISPR, Addgene gave to me: Functional Genetic Variants Revealed by Massively Parallel Precise Genome ...prime editing systems by manipulating cellular determinants of editing outcomes🎵 On the 5th day of CRISPR...CRISPR, Addgene gave to me: High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral...prime editor with enhanced editing efficiency in plants 🎵 ...

  24. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    Published
    Jan. 17, 2020, 3 p.m.
    ...photo wearing holiday sweaters and Santa hats. There was even a giant taco... Miller lab annual #DeckTheLab...their door. What a fun video of Mario, dressed as Santa, delivering gifts to the Mushroom Kingdom. Kravitz.... Getting in the spirit for the holiday season. Santa frog is coming to town. @Addgene @UMich_MCDB #christmastaco...

  25. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...enable sorting and selection of transduced cells. Grant Challen’s lab also created constitutive (pCMV-dCas9...fused LSD1 to x-Cas9(3.7). x-Cas9(3.7) is a SpCas9 mutant that exhibits increased PAM flexibility, and this...recruit epigenetic machinery - but is there an advantage to using direct epigenetic modifiers? As with ...will determine the tool that you should use. If you want to study the effects of one particular modification...tool would be your best bet. Another potential advantage of CRISPR epigenetic tools is their persistence...

  26. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...expression and largely defines the amount of recombinant protein that will be produced. Many commonly-...Tet-Off vectors (Tet-Advanced)” are the most recent variants of the Tet-Off system.  For more information on...the first 24 hours of culture, when glucose is abundant and ethanol concentrations are low, pADH1 displays...pADH1 activity. Researchers have engineered pADH1 variants that are less sensitive to ethanol, including ... transcription from the UAS promoter. This is important because it allows them to be used together for...

  27. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...Goldilocks Antibody: Guest blogger Ava Shipman explains how the Wu lab is screening over 3,000 antibodies for... to RUBY-red, a bright red pigment reporter for plants.  Cas7-11 and PASTE: Omar Abudayyeh and Jonathan...your own work!) If you've got a research story you want to share on our blog, please reach out to [email protected]...

  28. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...can save you significant time and money. For instance, this report shows that anterograde, monosynaptic...see from the community is your negative data. We want to make sure people are selecting AAVs that will...work was cited by others?)  Ready to submit?  Fantastic! The next 25 submissions will receive a 20% off...

  29. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ...introduction of the model antigen, Ovalbumin, into cell lines to study antigen-specific responses. Find...screens have been used to interrogate T cells, an important immune cell type, in more complex and physiological...for a screen that assessed genes that regulate antigen-specific CD8+ T cell responses to a murine tumor...

  30. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

    Type
    Blog Post
    Published
    April 5, 2016, 2:30 p.m.
    ...Instrumental Access participants. Like Seeding Labs, Addgene also feels it’s incredibly important for scientific...challenges. However, these same researchers face significant barriers to scientific discovery and career development...accelerate biology research in the labs they’re granted to, as well as shine a light on some of the great...
Showing: 541 - 570 of 885 results