We narrowed to 842 results for: Eras

...polyclonal antibodies are readily available from several sources, fewer sources of recombinant antibodies...appropriate buffer, it’s time to quantify it. There are several options to choose from depending on the time, reagents...2018). Aggregation can arise from a shift in temperature, pH, or salt concentration (Amin et. al., 2014... shipped to customers on ice, preventing any temperature shifts. Of course, we are also cautious during...

...set up a standard PCR reaction (primers, dNTPs, polymerase) using the supernatant of lysed bacteria as template...standard PCR reaction: combine template, primers, polymerase, and dNTPs and then incubate with a standard ...heating step of the PCR reaction. A standard Taq polymerase is sufficient. Controls: Controls can make ...them onto the gel. The figure above summarizes generalized expected results for the three primers previously...

...RuvC-like), and Cas12a has one (RuvC-like), TnpB also operates with a single RuvC-like domain. In simple terms..., viral vectors have been successfully used to generate gene-edited plants without the need for tissue... additional enzymes or domains (increasing the overall size), using TnpB as the core nuclease could help...Conclusion & Future Directions TnpB represents a new generation of compact, efficient, and versatile genome editing...

...Figure 1 for a visual overview! Figure 1: General overview of the MoClo assembly process to produce... common MoClo nomenclature. The overhangs are generated using type IIS restriction enzymes (BsaI, BbsI...MoClo plate, always do so in cold conditions and preferably on dry ice. Careful use of your glycerol stocks...plates. However, even though the MoClo system is generally very efficient and precise, positive colonies ...

... are several different versions of the plasmid partitioning system in the bacteria, but generally it consists...coresident plasmid. Asymmetric plasmid loss occurs for several reasons, including one plasmid blocking replication...compatible or not.  Using plasmid incompatibility for therapeutic advantage Although it may seem like plasmid incompatibility...

...multiple gRNAs into separate AAV vectors, increasing overall packaging capacity but necessitating purification...labeling, enrichment on streptavidin and next-generation sequencing). Using this sensitive method, they...that the field has overcome with split AAVs. In general split AAVs break a large transgene in two pieces...  Wang D, Zhang F, Gao G (2020) CRISPR-Based Therapeutic Genome Editing: Strategies and In Vivo Delivery...

...multi-drug resistance: definitions vary in the literature, but generally organisms with multi-drug resistance are...abstract, convoluted scientific concept. It is imperative that we all understand what antibiotic resistance...meats. We can focus on creating new antibiotic therapies, and investigate more effective drug targets (...

...quantitative imaging experiments and cell physiology in general. Pitfalls of Fluorescent Protein Fusions Observing...Previously, we have described a number of practical considerations that should be taken into account when deciding...cellular compartments other than the cytoplasm. Generally, FPs evolved in or were engineered for use in ...N-glycosylation consensus sequences in popular FPs. This generated a palette of inert green, cyan, yellow, and blue...

...different size and structural complexity. After several steps of tissue processing prior to running my ...voltage in the cytometer’s software in order to generate signals in a reasonable range. If colleagues in... being considered for the analysis. (C) A more liberal gate allows more cells to be considered for the...some like to make looser ones (Figure 3). A more “liberal” gate in the beginning should not have a great ...

...efficient method to generate precise, unmarked mutants, but it frequently requires considerable optimization ...transformation and plasmid curing steps, and can generate off-target mutations. Modifying bacterial genomes...clinical infectious diseases at the Massachusetts General Hospital where he is now an attending physician...

...of understanding how the body works, generating new therapeutics, and sometimes formulating new biological...Bio-protocol all work to provide more descriptive and interactive protocols that make it much easier to learn new...Initial positive results and the excitement they generate can, however, bias us. We’ve all heard stories...

...binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily...green, insert) are cut with restriction enzymes to generate compatible overhangs that allow them to bind. ...single tube allowing the compatible overhangs generated by restriction digestion to anneal to one another...midiprep or a maxiprep. In these purifications, you generally lyse the bacteria; add chemicals to precipitate...

...base editing from the first generation of base editors to the eighth generation (and dual base editors, too...been updating many of these posts over the last several months. Now the #12DaysOfCRISPR is back on Twitter...

...process, simply enter your preprint title or a general description of the deposit. Select your desired...millions. Pooled libraries must be verified by next-generation sequencing. Please see the help center article...that can be used under BSL1 conditions were generated in your lab are necessary for use of a plasmid...need information on how to verify any genomic alterations unique to your strain. Please contact us at deposit...

...Kit from John Chodera's Lab - Get high expression of His-tagged human kinases in bactera. Listen to Our...Listen to the entire Hot Plasmids episode! We generally publish 5-10 Hot Plasmid articles with each of...

...a Clinical Fellow in Pathology at Masachusetts General Hospital and Postdoctoral Fellow in the Church ...gene drives focusing on safety and the ability to generate mutant microbial strains faster than ever before... Advisory Board with a particular interest in generating Cas9 based tools to enable facile control of ...

...service to further contribute to our mission to “accelerate research and discovery.” Here are the details...in the research on the SARS-CoV-2. Nine of the overall plasmid selection will be open to industry scientists...preparation is quality controlled through next generation sequencing and spectrophotometry to test purity...

...researchers facilitate and accelerate scientific discovery. How can Addgene help accelerate your research? Let...Addgene headquarters every day. That would be an average of 673 plasmids each day. This requires tremendous...

...dual-nuclease Cas9-Cas9 chimeras Scot Wolfe’s lab has developed dual-nuclease Cas9-Cas9 chimeras with higher target... in Nature Communications Find the Cas9-Cas9 chimera plasmids A modular toolkit for genome editing...

...information that is entered at checkout is used to generate the legal agreement (MTA), which streamlines the...Pay by Purchase Order.” Enter the PO number you generated through your organization in this field. Please...purchasing department requires a quote in order to generate a PO, learn more about how to create a quote. ...

...Twenty years of accelerating scientific discovery. Over 150,000 plasmids empowering researchers worldwide...sequencing capabilities to complement our next-generation sequencing, setting new standards for plasmid... not just sharing research materials — we're accelerating the future of science. Want to learn more about...

...and in my personal experience, is a quick way to generate many mutated plasmid constructs. The second post...and discusses a newer, CRISPR-based technique to generate C->T or G->A transitions in a target sequence ...potential specificity issues, and other means of generating mutations in live cells are all discussed further...

... and PTPN2, which are of interest as cancer immunotherapy targets but are embryonic lethal, hindering ... titer lentivirus preparations than the first generation CHIME (pXPR_053). Find X-CHIME plasmids here!...., & Sharpe, A.H. (2019). PTPN2 regulates the generation of exhausted CD8+ T cell subpopulations and restrains...

...communicating science to the general public and training the next generation of science communicators. In... is a source of scientific information for the general public as well as a platform for scientists to ...

...languished in the pages of thick textbooks are now liberated to adorn the walls of universities and the front...Science is now being adapted to inspire to a new generation. Daniel Stoupin is a PhD scientist and photographer...aspect alone. They can study molecules, analyze terabytes of information on black screens, but not to know...

... Plasmids available for Packaged on Request consideration will have a banner on their material page. Interested... in-stock preps. If a request has technical considerations that may affect expected titers, a customer...review: 1–2 days Prep and QC completed: 6–8 weeks Average shipping time: 1–2 days (USA); 3–4 days (international...

...organizations to share materials more broadly to accelerate COVID-19 research: Sinai Health System                                     ... available to industry? Over the past 8 years, several organizations have made their plasmids available...Shop’ for Fluorescent Proteins, and plasmids from several labs at Florida State University became openly ...

...back from other summer programs. Amy took into consideration just how busy I was and ensured that my decision...and the insert size should be a max of 500 bp (preferably). I compiled a list of three options from the...Through Addgene, I learned the basics of lab safety, general work skills — such as using Google workspace — ...

...needs to integrate within an exon of a gene to generate a transcriptional fusion. This may disrupt the...reading frame, in fact, they can be activated a considerable distance away from a gene which can make identification...there are many ways to insert your construct to generate a collection of monitored genes and mutants - ...

...seqWell and going into detail about how we use next generation sequencing results to perform quality control... We’ve also talked about how our new Snapgene generated maps provide improved feature detection with an...is always to send prepped plasmid DNA for next generation sequencing. We then copy the contents of the ...