Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 834 results for: Eras

Showing: 541 - 570 of 834 results

  1. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...quantitative imaging experiments and cell physiology in general. Pitfalls of Fluorescent Protein Fusions Observing...Previously, we have described a number of practical considerations that should be taken into account when deciding...cellular compartments other than the cytoplasm. Generally, FPs evolved in or were engineered for use in ...N-glycosylation consensus sequences in popular FPs. This generated a palette of inert green, cyan, yellow, and blue...

  2. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...different size and structural complexity. After several steps of tissue processing prior to running my ...voltage in the cytometer’s software in order to generate signals in a reasonable range. If colleagues in... being considered for the analysis. (C) A more liberal gate allows more cells to be considered for the...some like to make looser ones (Figure 3). A more “liberal” gate in the beginning should not have a great ...

  3. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...efficient method to generate precise, unmarked mutants, but it frequently requires considerable optimization ...transformation and plasmid curing steps, and can generate off-target mutations. Modifying bacterial genomes...clinical infectious diseases at the Massachusetts General Hospital where he is now an attending physician...

  4. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...of understanding how the body works, generating new therapeutics, and sometimes formulating new biological...Bio-protocol all work to provide more descriptive and interactive protocols that make it much easier to learn new...Initial positive results and the excitement they generate can, however, bias us. We’ve all heard stories...

  5. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily...green, insert) are cut with restriction enzymes to generate compatible overhangs that allow them to bind. ...single tube allowing the compatible overhangs generated by restriction digestion to anneal to one another...midiprep or a maxiprep. In these purifications, you generally lyse the bacteria; add chemicals to precipitate...

  6. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ...base editing from the first generation of base editors to the eighth generation (and dual base editors, too...been updating many of these posts over the last several months. Now the #12DaysOfCRISPR is back on Twitter...

  7. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...process, simply enter your preprint title or a general description of the deposit. Select your desired...millions. Pooled libraries must be verified by next-generation sequencing. Please see the help center article...that can be used under BSL1 conditions were generated in your lab are necessary for use of a plasmid...need information on how to verify any genomic alterations unique to your strain. Please contact us at deposit...

  8. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    Published
    Nov. 15, 2016, 3:30 p.m.
    ...a Clinical Fellow in Pathology at Masachusetts General Hospital and Postdoctoral Fellow in the Church ...gene drives focusing on safety and the ability to generate mutant microbial strains faster than ever before... Advisory Board with a particular interest in generating Cas9 based tools to enable facile control of ...

  9. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...Kit from John Chodera's Lab - Get high expression of His-tagged human kinases in bactera. Listen to Our...Listen to the entire Hot Plasmids episode! We generally publish 5-10 Hot Plasmid articles with each of...

  10. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    Published
    July 28, 2020, 1:15 p.m.
    ...service to further contribute to our mission to “accelerate research and discovery.” Here are the details...in the research on the SARS-CoV-2. Nine of the overall plasmid selection will be open to industry scientists...preparation is quality controlled through next generation sequencing and spectrophotometry to test purity...

  11. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ...researchers facilitate and accelerate scientific discovery. How can Addgene help accelerate your research? Let...Addgene headquarters every day. That would be an average of 673 plasmids each day. This requires tremendous...

  12. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ...dual-nuclease Cas9-Cas9 chimeras Scot Wolfe’s lab has developed dual-nuclease Cas9-Cas9 chimeras with higher target... in Nature Communications Find the Cas9-Cas9 chimera plasmids A modular toolkit for genome editing...

  13. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...information that is entered at checkout is used to generate the legal agreement (MTA), which streamlines the...Pay by Purchase Order.” Enter the PO number you generated through your organization in this field. Please...purchasing department requires a quote in order to generate a PO, learn more about how to create a quote. ...

  14. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...Twenty years of accelerating scientific discovery. Over 150,000 plasmids empowering researchers worldwide...sequencing capabilities to complement our next-generation sequencing, setting new standards for plasmid... not just sharing research materials — we're accelerating the future of science. Want to learn more about...

  15. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...and in my personal experience, is a quick way to generate many mutated plasmid constructs. The second post...and discusses a newer, CRISPR-based technique to generate C->T or G->A transitions in a target sequence ...potential specificity issues, and other means of generating mutations in live cells are all discussed further...

  16. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ... and PTPN2, which are of interest as cancer immunotherapy targets but are embryonic lethal, hindering ... titer lentivirus preparations than the first generation CHIME (pXPR_053). Find X-CHIME plasmids here!...., & Sharpe, A.H. (2019). PTPN2 regulates the generation of exhausted CD8+ T cell subpopulations and restrains...

  17. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ...communicating science to the general public and training the next generation of science communicators. In... is a source of scientific information for the general public as well as a platform for scientists to ...

  18. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...languished in the pages of thick textbooks are now liberated to adorn the walls of universities and the front...Science is now being adapted to inspire to a new generation. Daniel Stoupin is a PhD scientist and photographer...aspect alone. They can study molecules, analyze terabytes of information on black screens, but not to know...

  19. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ... Plasmids available for Packaged on Request consideration will have a banner on their material page. Interested... in-stock preps. If a request has technical considerations that may affect expected titers, a customer...review: 1–2 days Prep and QC completed: 6–8 weeks Average shipping time: 1–2 days (USA); 3–4 days (international...

  20. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    Published
    June 23, 2020, 1:15 p.m.
    ...organizations to share materials more broadly to accelerate COVID-19 research: Sinai Health System                                     ... available to industry? Over the past 8 years, several organizations have made their plasmids available...Shop’ for Fluorescent Proteins, and plasmids from several labs at Florida State University became openly ...

  21. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...back from other summer programs. Amy took into consideration just how busy I was and ensured that my decision...and the insert size should be a max of 500 bp (preferably). I compiled a list of three options from the...Through Addgene, I learned the basics of lab safety, general work skills — such as using Google workspace — ...

  22. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...needs to integrate within an exon of a gene to generate a transcriptional fusion. This may disrupt the...reading frame, in fact, they can be activated a considerable distance away from a gene which can make identification...there are many ways to insert your construct to generate a collection of monitored genes and mutants - ...

  23. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...developed by Thomas Clandinin’s lab, and incorporates several common genetic tools in Drosophila (Fig. 1):  ...Ribozyme), a self-cleaving ribozyme. In the second-generation SPARC2 system, HDVR is placed upstream of the...organisms including mouse and fish, SPARC is likely generalizable to other systems as well. References: del...

  25. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    Published
    Feb. 1, 2018, 3:07 p.m.
    ...seqWell and going into detail about how we use next generation sequencing results to perform quality control... We’ve also talked about how our new Snapgene generated maps provide improved feature detection with an...is always to send prepped plasmid DNA for next generation sequencing. We then copy the contents of the ...

  26. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...familiar with the alarming amount of waste that is generated. This is a systemic problem that we need to address...account for an estimated 2% of global plastic generation. Less than 9% of plastics are ever recycled, ...boxes). We get these boxes a lot when receiving temperature-controlled reagents. Expanded polystyrene will...

  27. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...associated with the brain’s reward circuits are generally linked to dopamine (DA), a well-known reward chemical...more mysterious. Thus, scientists have developed several ways to sense dopamine. Methods for measuring Dopamine...Pavlovian conditioning Find the GRABDA sensor here! Overall these sensors are powerful new developments in ...

  28. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...EF1ɑ-driven viral vectors (AAV1) - and you can also find several other constructs in plasmid form. Fig. 1...neuroscience-related proteins, but we recently added several broadly useful antibodies for detecting protein... of the human proto-oncogene c-Myc. Since the generation of the first anti-c-Myc [9E10] (Even, et al.,...

  30. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...inefficient process– just 1 out of 10,000 cells on average! Without some means of quickly determining which...resistance!  What are antibiotics? Antibiotics are generally defined as agents that kill bacteria, or inhibit...lab, their mechanism for killing bacteria, and general working concentrations. For instructions on how...
Showing: 541 - 570 of 834 results