Skip to main content
Addgene
Showing: 541 - 570 of 758 results

  1. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...of ccdB, leading to positive identification of desired clones.   ...Becomes a powerful cloning tool... efficiently, as those that do not contain the desired insert should not grow.   Figure 2: Image...

  2. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...available for a large number of targets, and can require specific conjugates. And, of course, once you have...either re-develop or re-optimize your assay for an entirely new affinity reagent.    Fig. 1: A comparison...

  3. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...and the lab, you may not be able to carry out the entire problem solving process on your own, but you can...skillset is a part of your toolkit is important. Paired with your other skills, both technical and transferable...

  5. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...scientists can receive recognition for their efforts, inspire future research, and increase their impact. Open...’ comments, and author responses can be shared directly alongside the preprint while the work is still...

  6. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...targeting efficiency of only 27.9% and homology-directed repair (HDR) taking place in 41.2% of edited cells...NHEJ rather than HDR. Figure 1: Homology-directed repair in targeted zygotes proceeds from the maternal...

  8. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...Therefore, co-infection with a helper adenovirus is required to provide the viral proteins in trans. The Gutless...virus. Purification (2 days) - Purification is required if rAdV is to be used in vivo. The standard method...

  10. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...genotype each animal to identify the ones with the desired genotype. Inducible and conditional systems Conditional...conditional knockout mouse, loxP sites are inserted directly into the genome to flank the gene that will be...

  11. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...Just like with any sport, hours of practice are required and no one lab or professor becomes an overnight...they are looking for in grant submissions and will direct you on how to submit your work either through seminars...

  12. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... elegans with CRISPR can be difficult since it requires the much less efficient double stranded templates...cell cycle. Cells that had IL-3 withdrawn became entirely red as they could not proliferate. Eastman et...

  13. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...antibody affinity: stronger binding antibodies will require harsher stripping to remove, so start with the ...with caution. Unlike PVDF, they can be returned directly to buffer without a re-wetting step. Important...

  14. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...for your next experiments, they will also likely inspire further-improved versions. Thanks to Christophe...Leterrier, whose FocalPlanes piece on mStayGold inspired and informed this section.  Find mStayGold plasmids...

  15. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...reverse transcriptase (RT) template including the desired edit. Figure 1: Prime Editing schematic....guide Find CRISPR plasmids by function Browse our entire plasmid collection ...

  16. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...functional loss (Ho et al. 2014). To overcome this, paired gRNAs can be used to target specific sizes of a...visualizing ncRNA localization and function, but directly incorporating very long ncRNAs is impractical....

  17. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... inhibitor response 1 (TIR1) expressed under a desired promoter with a specific spatiotemporal expression...motif (PAM). They're used to determine the PAM requirements of Cas enzymes. CRISPR-ARE is a new system from...

  18. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...strategy helps your sections stand out, and makes the entire poster more navigable.  Choose your content wisely... that links readers to more information if they desire it. Shapes. In this type of poster, written and...

  19. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...capabilities. Specifically this includes primer directed isothermal RNA amplification, new toehold switch...samples; we showed that we can detect the virus directly from infected monkey blood plasma without purification...

  20. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...suggestions and take your project in the wrong direction. The best way to avoid miscommunication is to ...proactive person that all employers are eager to hire. An excellent resource to learn more about assertiveness...

  21. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...their academic science careers in order to head to Ireland and take a chance as entrepreneurs. To make things...in fact, our color changing flower project was inspired by a Facebook comment.  In our day job, we worked...

  22. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...poster: those that want you to walk them through the entire poster, or those that want to look through themselves...handle on your nametag for the tweep up (and for the entire conference, in general). After the conference Review...

  25. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    .... A special class of FPs, the FP timers, add an entire new dimension to monitoring localization; using...emission and absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers...

  26. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis. Nature Plants,..., M. D., Wu, J. C., & Garcia-Marcos, M. (2024). Direct interrogation of context-dependent GPCR activity...

  27. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...in the depositing lab, although you may have an entirely different use in mind! This section of our plasmid...in the E. coli lab strain DH5a, but others may require strains such as ccdB survival (for Gateway plasmids...

  28. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ..., the drive could eventually spread through the entire wild population with unpredictable ecological effects...ecological, and reproductive confinement typically require very little effort to implement (depending on the...

  29. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...tools work in any plant? Patron: The MoClo kit is entirely for dicots, not grasses and wheat, but we do work... Patron: I started my Twitter account recently inspired by an academic in literature who gave a talk to...

  30. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...emission is green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors...many FPs (particularly those derived from GFP) requires oxygen. Therefore, these FPs cannot be used in...
Showing: 541 - 570 of 758 results