We narrowed to 798 results for: KIN

.... There’s also tips for setting writing goals, tracking progress, and starting a writing group. Loop Habit...literally a tomato! Image from Gerlos.   Tools for checking grammar and jargon Hemingway Editor  This is a...

...Incredibly imaginative, funny, bittersweet, and shocking at times."     - Jason Snair, Product Designer...on today! Addgene Book Club (ABC) founder, Eric Perkins, is Addgene’s Director of Science Product Management...

...contrast” variant provides improved detection for tracking large populations of neurons in wide-field imaging...fusion fluorescent proteins can affect DREADD trafficking and localization. The Sternson lab recently deposited...

...interpreting human variation at large scales and making personalized medicine a reality,” Chiasson explains...replicate current knowledge, but the sensor can also kinetically outperform current methods. Unger plans to use...

...Wang, Y., Missinato, M. A., Onuoha, E., Perkins, L. A., Watkins, S. C., . . . Bruchez, M. P. (2016). A ...

...researchers to utilize and develop these tools by making their own fluorescent protein technologies widely...learn more about Xin and Fatima's work and keep checking back in with the Addgene blog for future award...

...and how it can be used in a lab? Here we’ll be talking about the ins and outs of this classification system...negative (red/pink) staining. Image credit: Mark Perkins https://www.flickr.com/photos/occbio/7092385559...

...effectiveness of CRISPR/Cas9, scientists are still looking for ways to diminish the possibility of those off-target...s Disease.” Journal of Alzheimer's disease & Parkinsonism. https://doi.org/10.4172/2161-0460.1000439 6...

...diseases in which sequence replacement holds some kind of fitness advantage but error-prone repair of the... the gene would be disastrous (i.e. if breaking the gene is worse than leaving the mutation alone). CRISPR...

...box above. The number of action potential (AP) spiking events are indicated below the trace; asterisk ... now available through Addgene. Whether you're working with cell cultures, slice preparations, or in vivo...

... light Chemical reaction High-energy photons Kinetics of photon generation Slower Faster Cofactors...and more. These variant proteins offer different kinetics, substrate requirements, and photon emission wavelengths...

... those first. Depending on the markers you are looking at and the antibodies you are using, you may need... general consensus seems to be that if you are looking for a rare population or are unsure what amount...

...Addgene was built on the concept of accessibility: making plasmids more accessible to scientists around the...helpfully. You and your readers are now a team, working together to share information. Sometimes it is ...

...useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource displays...they're available through Addgene. So whether you're looking for the exact Cas9 plasmid used by Jinek et al....

...proteins and mutations in the trs site reduce Rep nicking. Since Rep proteins are important for viral genome...PMID: 28967288. Shitik, E. M., Shalik, I. K., & Yudkin, D. V. (2023). AAV- based vector improvements unrelated...

...FRET-based sensors such as Twitch. While calcium is king in the sensor world, there are sensors available...expression and downstream monitoring. Nervous about making a virus for your biosensor? Don’t be! We have you...

...replicate and spread. Instead, viruses replicate by hijacking the host’s system to produce more virus and continue...viral applications to help you start (safely!) thinking about the possibilities. Ready to plan your next...

... noise in the process of sampling and inference-making. Even perfect transparent and rigorous studies ...significant result that may be the result of noise, p-hacking, or unclear methods. Many thanks to our guest ...

...initiates the extracellular signaling-regulated kinase (ERK) signaling cascade. This signaling is a lot...signaling by using a fluorescent reporter for Erk kinase translocation. These results demonstrate that OptoNBs...

...efficiency. Check out Addgene’s blog to learn about making your own competent cells and our protocols page...passed down through cell division. You can find all kinds of different lentiviral and AAV plasmids as well...

...however can interfere with other genetic pathways making it difficult to link a phenotype to the gene being...TurboID and miniTurbo Article contributed by Eric Perkins  Listen to the TurboID and miniTurbo podcast segment...

...information on this system! GPCR-APEX: Interested in tracking the downstream signaling pathways mediated by ... JA, Gygi SP, Kruse AC (2017) Multidimensional Tracking of GPCR Signaling via Peroxidase-Catalyzed Proximity...

...performed to amplify the target using PCR. Instead of tracking the reaction progress as in real time PCR, each...introduced how digital PCR works, let’s explain what kind of assays can be used to sniff out your desired ...

... and careful Addgenies, but also a lot of good tracking. Our Software and IT Teams made major upgrades...plasmids in the repository. The Outreach Team has been making sure scientists know about the new initiative and...

...behind. While commercial kits are available for making small point mutations, large deletions/insertions...-12 kb range. REPLACR-mutagenesis also requires making your own electrocompetent cells in which the phage...

... blue, indicating that your IPTG and x-gal are working as they should be. Don't rush the process: It...between blue and white colonies. Take care in making your plates: X-gal is light and temperature sensitive...

...conserved cellular machinery to process gRNAs. Hijacking tRNA processing to make gRNAs In a recently published...Cas toolkit in human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22...

...of the same technical advantages that come with working with prokaryotes. This includes but is not limited...selection rather than antibiotic selection when working with yeast, due to high rates of spontaneously ...

... cardiovascular and metabolic diseases. We are knocking out genes that have been implicated in disease...about the history. How had investigators done this kind of work before? Musunuru: In the "olden days," not...

...people knew how to access Addgene, and that we were making it possible for scientists to both deposit and ...through physical outreach, visiting organizations, knocking on doors, and attending conferences. And when ...