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  3. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ... Anti-GFP [N86/8R] page for more details. Image credit: Addgene.   Find anti-GFP [N86/8R] here!   ...

  4. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...discuss the process, which eventually led to the creation of a standardized agreement on the transfer of...

  10. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...flexibility of AAV genome design in remarkably creative ways. The continued development of more and smaller...

  12. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ... IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

  16. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...cells but with far more predictability. The beads create an artificial positive (bound) and negative (unbound...

  20. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...neurodegeneration. The level of this protein is decreased in Alzheimer’s disease patient’s brains. However...

  24. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...limitations. New efforts have recently been made to increase cutting efficiency and diversify PAM sequences...

  27. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...protein-protein interactions with other peptides. Created with BioRender.com.     Many biologists can...

  28. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ... double-edged sword, however, and has greatly increased the burden associated with identifying plasmids...

  30. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ... How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has Been Used for Lineage...

  31. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...Well, it used to be a lab assistant, but with increased plasmid demand it was too time consuming to complete...

  34. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...hard during this talk since you’ll be sharing your screen and won’t be able to see who’s asking a question...

  36. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...starting vector that the depositing lab modified to create their new plasmid.  The Addgene Vector Database...

  37. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...making their surrounding media acidic through the secretion of lactic acid (a by-product of cellular metabolism...

  38. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https...

  39. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation...

  40. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...17,560 CRISPR plasmids. That’s almost a ninety-fold increase in size! From this collection, we’ve shared almost...
Showing: 721 - 760 of 788 results