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  2. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...with a plasmid that has multiple cloning sites flanking the multicistronic element(s), or you could replace...

  3. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...collection (nr/nt)” as it contains a composite of GenBank, EMBL, DDBJ, and PDB sequences and may be the most...

  4. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...your work, head over to greenfluorescentblog. Thank You to Our Guest Blogger! Gal Haimovich, PhD, is...

  6. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...Check out the NIH’s Developmental Studies Hybridoma Bank (DSHB). 1 ml of antibody-containing hybridoma supernatant...

  8. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like LwaCas13a...

  10. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...homologous sequences to the insert by PCR. Many thanks to our guest bloggers Jake Watson and Javier García-Nafría...
Showing: 361 - 380 of 441 results