We narrowed to 447 results for: Ank

1. The AAVantages of AAV in CRISPR Screens

   Type

   Blog Post

   Published

   Feb. 4, 2025, 2:15 p.m.

   ...have gotten larger, easier, and more comprehensive thanks to the consistent development and improvement of...

2. Plasmids 101: Degron Tags

   Type

   Blog Post

   Published

   March 14, 2024, 1:15 p.m.

   ...structures, interacting partners, and localization. Thankfully, this is a hot topic with many resources! In ...

3. Tips for a 1st time AAV user (by a Rookie AAV user)

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   Blog Post

   Published

   Oct. 23, 2018, 11:49 a.m.

   ...Please share them in the comments below! Special thanks for Dr. Dan Stone and Harshana de Silva Feelixge...

4. 3D Printing Meets CRISPR Cas9

   Type

   Blog Post

   Published

   Oct. 17, 2017, 12:55 p.m.

   ...make the molecular world real for students. Many thanks to our guest blogger Tim Herman! TIm Herman is ...

5. Google Forums Round Up: First Impressions of NgAgo

   Type

   Blog Post

   Published

   Aug. 4, 2016, 2:30 p.m.

   ...Other Argonaute Plasmids from the Doudna Lab Many thanks to our Guest Blogger Pooran Dewari! Pooran Dewari...

6. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

   Type

   Blog Post

   Published

   April 19, 2016, 2:30 p.m.

   ...your chances of finding artifactual results. Many thanks to our guest blogger Erik L. Snapp! Erik Lee Snapp...

7. CRISPR 101: Mammalian Expression Systems and Delivery Methods

   Type

   Blog Post

   Published

   Sept. 24, 2015, 2:30 p.m.

   ...component delivery with validated tools.  Special thanks to Joel McDade for creating the Expression Systems...

8. Plasmids 101: Multicistronic Vectors

   Type

   Blog Post

   Published

   Sept. 9, 2014, 8:20 p.m.

   ...with a plasmid that has multiple cloning sites flanking the multicistronic element(s), or you could replace...

9. Tips for Using BLAST to Verify Plasmids

   Type

   Blog Post

   Published

   May 29, 2014, 1:29 p.m.

   ...collection (nr/nt)” as it contains a composite of GenBank, EMBL, DDBJ, and PDB sequences and may be the most...

10. Which Fluorescent Protein Should I Use?

    Type

    Blog Post

    Published

    May 20, 2014, 2:06 p.m.

    ...your work, head over to greenfluorescentblog. Thank You to Our Guest Blogger! Gal Haimovich, PhD, is...

11. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    Published

    Aug. 27, 2020, 1:15 p.m.

    ...dynamics of PPIs are the focus of study. Many thanks to our guest blogger Patrick Miller-Rhodes from...

12. CRISPR 101: RNA Editing with Cas13

    Type

    Blog Post

    Published

    July 31, 2020, 12:30 p.m.

    ...prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like LwaCas13a...

13. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type

    Blog Post

    Published

    July 16, 2020, 1:15 p.m.

    ... results for China with more uncertainty. Many thanks to our guest bloggers Max W. Shen from MIT, Alvin...

14. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    Published

    Sept. 28, 2017, 2:01 p.m.

    ... In this special CREATE cap plasmid, loxP sites flank the downstream polyA sequence. When viruses produced...

15. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    Published

    Feb. 27, 2018, 2:04 p.m.

    ...Check out the NIH’s Developmental Studies Hybridoma Bank (DSHB). 1 ml of antibody-containing hybridoma supernatant...

16. Transferable Skills Guide: Identifying Your Transferable Skills

    Type

    Blog Post

    Published

    Jan. 11, 2018, 3:29 p.m.

    ...from a client you helped. Maybe you awkwardly say thanks and move on with your day, feeling good but focused...

17. Summer SciComm Series: Modes of Communication

    Type

    Blog Post

    Published

    July 1, 2022, 1:15 p.m.

    ...to choose  Where is your audience?  It’s, quite frankly, much easier to go where your audience is than ...

18. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    Published

    Oct. 10, 2023, 1 p.m.

    ...in bacteria that express the Tn5 element, but thankfully, most competent cells do not express this factor...

19. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    Published

    Oct. 18, 2018, 12:37 p.m.

    ...homologous sequences to the insert by PCR. Many thanks to our guest bloggers Jake Watson and Javier García-Nafría...

20. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    Published

    Aug. 9, 2016, 2:30 p.m.

    ...-L 9 -N 1 -U GRNA_23MERS -S GRNA_HITS.sam Many thanks to our guest blogger, Aneesh Karve! Aneesh Karve...