We narrowed to 566 results for: FIE
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TypeBlog PostPublishedApril 27, 2017, 2:30 p.m....SITN team brings in scientists from a variety of fields as well as students from the ed school, design ...
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Seeing Red: Simple GFP Photoconversion
TypeBlog PostPublishedAug. 4, 2015, 2:30 p.m....had turned red. They subsequently confirmed that purified GFP could be imaged in the green state after excitation... -
Science as Art
TypeBlog PostPublishedMarch 2, 2015, 3:13 p.m....etc. Photography is critically important in many fields of biology and I believe it could have made the... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog PostPublishedFeb. 18, 2015, 3:09 p.m..... Biallelically disrupted cells can be easily identified. Even in the case of clones with monoallelic ... -
Inside Addgene's 10 Year Anniversary Gala
TypeBlog PostPublishedJuly 3, 2014, 3:48 p.m....of requesting and sharing plasmids has become simplified through Addgene. Looking back, Addgene’s journey... -
Working 9-5: an Addgene Co-op
TypeBlog PostPublishedJune 2, 2022, 2:15 p.m....majority spend two to three) working in your chosen field. This cooperative education experience (co-op) is... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog PostPublishedJune 8, 2023, 1:15 p.m....can be used with many conventional microscopes, amplified staining methods, and oligonucleotide-based staining... -
Addgene is Expanding Our Viral Vector Service!
TypeBlog PostPublishedOct. 15, 2024, 1:15 p.m....produced, the request will be denied, and you will be notified via email. There is no charge for the request ... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog PostPublishedJuly 9, 2015, 2:30 p.m....resumes The cover letter must address how you are qualified and why you are interested specifically in the... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog PostPublishedSept. 15, 2014, 6:05 p.m....their labs studying the mechanisms of RNAi, the field for which Craig Mello and Andrew Fire won the Nobel... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog PostPublishedAug. 13, 2014, 3:49 p.m....affibodies to enhance magnetic cancer cell capture. Fierer et al (Proc Natl Acad Sci U S A. 2014 Mar 17. PubMed... -
The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins
TypeBlog PostPublishedJuly 29, 2014, 6:16 p.m....go-to place. In 2012, his National High Magnetic Field Lab worked with an impressive 1,350 scientists from... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog PostPublishedDec. 3, 2020, 2:15 p.m....occurring AAV serotypes that have thus far been identified: AAV1-11. Nine regions of variability in the ... -
Plasmids 101: What is a plasmid?
TypeBlog PostPublishedApril 2, 2020, 6:17 p.m....innovative solutions necessary for advancing the field. What is a plasmid? At their most basic level... -
Hot Plasmids - October 2022
TypeBlog PostPublishedOct. 6, 2022, 1:15 p.m....from the labs of Mingshan Xue and John Spudich identifies a novel class of light-gated potassium channelrhodopsins... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog PostPublishedFeb. 13, 2018, 2:51 p.m....at 37 °C. These short DNA fragments can then be purified for subsequent library preparation and high-throughput... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog PostPublishedFeb. 1, 2018, 3:07 p.m....Aligning sequences with Snapgene Once we’ve identified the features within a plasmid, we next verify... -
An Addgene Co-op: The Intersection of Fun, Friendship, and Work
TypeBlog PostPublishedJune 29, 2023, 7 p.m.... position/role, was both knowledgeable in their field and eager to engage with the work of others. Having... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog PostPublishedMay 2, 2023, 1 p.m....world, approximately 50% of predicted genes have unidentified roles as well (Vanni, et al). Is it true that... -
Bricking Science: Portraying Scientific Reality Through LEGO
TypeBlog PostPublishedNov. 29, 2016, 3:30 p.m....gmail.com. Who knows, your story might just get “legofied.” Many thanks to our guest blogger, Dalila Cunha...