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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 610 results for: REV

Showing: 361 - 380 of 610 results

  1. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ... anneal to the backbone in either a forward or reverse orientation so you’ll need some way to verify that...digestion, you will need to use a phosphatase to prevent re-circularization of the backbone plasmid (see...

  2. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ... should review before using an antibody.  Antibodies and Reproducibility First, let’s review why validation...

  3. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...really does not take much time at all. Much of the previous literature using AAVs for DREADD delivery indicates... of neurons showing a very specific phenotype. Previous neural tracing methods used viruses that were ...

  5. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...preparation, staining procedures, and controls. To prevent instrument clogs, ensure that samples are single... LA (1972) Fluorescence Activated Cell Sorting. Review of Scientific Instruments 43:404–409 . https://...

  6. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...evolutionary classification of CRISPR–Cas systems. Nature Reviews Microbiology, 13(11), 722–736. https://doi.org/... burst of class 2 and derived variants. Nature Reviews Microbiology, 18(2), 67–83. https://doi.org/10.1038...

  7. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...excitation. After a short period, the electrons revert to a lower energy state, whereby a photon is emitted...markers they label) can occur. As I mentioned previously, you can also use fluorescent reporters, such...

  8. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...super-resolution imaging of ER and mitochondria reveals dynamic organelle interactions. a) Labeling scheme...filament assembly-regulating proteins in vitro reveals complex events. a) Micrographs of a fluorescently-labeled...

  9. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...Single Molecule Localization (PALM/STORM), or Reversible Saturable Optical Linear Fluorescence Transitions.... Lens-based fluorescence nanoscopy. Quarterly reviews of biophysics 48, 178-243 (2015). PubMed PMID: ...

  10. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...the original reference library, as well as GC%, reverse compliment sequences, and more. Output_Compact:...Wang, Tim, et al. "Gene essentiality profiling reveals gene networks and synthetic lethal interactions...

  11. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ... stem cells: reprogramming à la carte." Nature Reviews Genetics 12, no. 4 (2011): 231-42. PubMed PMID:...reference. 1. Malik, Nasir, and Mahendra S. Rao. "A Review of the Methods for Human iPSC Derivation." Methods...

  12. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...primers: EMX1-Forward: CCATCCCCTTCTGTGAATGT EMX1-Reverse: GGAGATTGGAGACACGGAGA Q16: Why does the PX260 ...D., Lin, C., Gootenberg, J. S., Konermann, S., Trevino, A. E., Scott, D. A., Inoue, A., Matoba, S., Zhang...

  14. 2022 Depositor Week

    Type
    Blog Post
    Published
    Dec. 12, 2022, 2:15 p.m.
    ...depositing their data with us through our newly revamped Data Hub! The community needs more data-sharing...

  15. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...Addgene gave to me: Functional Genetic Variants Revealed by Massively Parallel Precise Genome Editing (...

  17. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...on CRISPR and synBio plasmid sharing In a recent review on CRISPR technology, for example, the authors ...

  18. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ..., the Phuels lab deposited a plasmid that will forever change the fly lab experience. The Frequency Light...

  20. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...evolving, but sometimes a technology is so useful it forever enhances biological research and discovery. CRISPR...
Showing: 361 - 380 of 610 results