We narrowed to 481 results for: SEC

...your favorite sequencing tricks in the comments section below.  Additional Resources on the Addgene Blog...

...divide the viral elements among multiple plasmids. Second generation packaging systems use 3 plasmids; an...

... accommodate up to 8.2 kb of transgene DNA. The second generation vectors are deficient in E1, E2, E3,...

...these high levels of viral replication are toxic. Second, infecting subsequently higher and higher order...

...embryology techniques such as basic observation and dissections. Students don’t usually perform more modern techniques...

...luciferase, cre/lox, yeast expression, worm expression, insect expression, plant expression, mouse targeting, ...

... our collection. This standardization provides security for requestors, ensuring that the plasmid they...

...different lengths and sequences, as unintended secondary structures in your pegRNA could inhibit editing...

...resistance genes to help cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids...

...PMC5693259.  Wang, Han, et al. "Split cGAL, an intersectional strategy using a split intein for refined spatiotemporal...

...engineered mouse seems less overwhelming! In the second blog post of this series, I’ll discuss crossing...

...our sequencing results in the Depositor Comments section at the bottom of the plasmid page. Viral preps...

...Maybe you ask for questions after completing each section of your talk. Some video conferencing software ...

...TadA from the complex the researchers developed a second set: The ABE8.x-d version carries a fusion of ...

...behaviors are available from Addgene and other sources; second, characterizing a part or optimizing a TU is much...

...sequence, including propensity for off-targets, secondary structures, etc., as well as the presence of a...

...fundamental to muscle and nerve function, hormone secretion, blood clotting, and as we all know, a structural...

...promoter to drive expression of Cre-mCherry and a second promoter to drive expression of a Cre-dependent...

...experiments performed will be useful in perpetuity.  Second, plasmids are easy to store and share, making recombinant...

... At least at Addgene, the questions in our FAQ section are really, truly frequently asked by customers...