We narrowed to 468 results for: SEC

1. Design Tips for Prime Editing

   Type

   Blog Post

   Published

   Jan. 23, 2025, 2:15 p.m.

   ...different lengths and sequences, as unintended secondary structures in your pegRNA could inhibit editing...

2. Plasmids 101: The Wide World of Natural Plasmids

   Type

   Blog Post

   Published

   Oct. 8, 2024, 1:15 p.m.

   ...resistance genes to help cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids...

3. Overcoming the Challenges of Lentiviral Production

   Type

   Blog Post

   Published

   Aug. 7, 2018, 12:31 p.m.

   ...divide the viral elements among multiple plasmids. Second generation packaging systems use 3 plasmids; an...

4. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   Published

   June 14, 2018, 11:44 a.m.

   ... accommodate up to 8.2 kb of transgene DNA. The second generation vectors are deficient in E1, E2, E3,...

5. Rabies and Neuronal Tracing

   Type

   Blog Post

   Published

   May 29, 2018, 1:51 p.m.

   ...these high levels of viral replication are toxic. Second, infecting subsequently higher and higher order...

6. Educational Planarian Tools for Teaching Developmental Biology Techniques

   Type

   Blog Post

   Published

   May 24, 2018, 1:20 p.m.

   ...embryology techniques such as basic observation and dissections. Students don’t usually perform more modern techniques...

7. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

   Type

   Blog Post

   Published

   May 4, 2021, 1:15 p.m.

   ...experiments performed will be useful in perpetuity.  Second, plasmids are easy to store and share, making recombinant...

8. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

   Type

   Blog Post

   Published

   April 6, 2021, 12:30 p.m.

   ...our sequencing results in the Depositor Comments section at the bottom of the plasmid page. Viral preps...

9. Viral Vectors 101: Calcium Sensors

   Type

   Blog Post

   Published

   Aug. 10, 2023, 1 p.m.

   ...fundamental to muscle and nerve function, hormone secretion, blood clotting, and as we all know, a structural...

10. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type

    Blog Post

    Published

    May 7, 2020, 1:15 p.m.

    ...TadA from the complex the researchers developed a second set: The ABE8.x-d version carries a fusion of ...

11. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type

    Blog Post

    Published

    March 12, 2020, 1:15 p.m.

    ...promoter to drive expression of Cre-mCherry and a second promoter to drive expression of a Cre-dependent...

12. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ...luciferase, cre/lox, yeast expression, worm expression, insect expression, plant expression, mouse targeting, ...

13. Tips for Technical Support Calls

    Type

    Blog Post

    Published

    Sept. 20, 2016, 2:39 p.m.

    ... At least at Addgene, the questions in our FAQ section are really, truly frequently asked by customers...

14. Adapting Toehold Switches to Detect Zika Virus

    Type

    Blog Post

    Published

    Aug. 30, 2016, 2:30 p.m.

    ...virtually any RNA sequence (Green et al, 2014). The second was an in vitro paper-based platform that allowed...

15. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ... to cleave again and possibly mediate HDR in a “second chance” mechanism. To prevent new editing post-...

16. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...Laureates and the ever expanding importance of RNA The second keynote lecture was presented by Nobel Laureate...

17. The Materials Science of Optogenetics Experiments

    Type

    Blog Post

    Published

    Sept. 17, 2015, 2:30 p.m.

    ...preparing the implant itself, which brings me to the second key point; the fiber optics need to be polished...

18. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    Published

    Aug. 6, 2015, 2:30 p.m.

    ...as things came up. For example, I updated some sections of the NPA’s website. By volunteering, you can...

19. How-to: 5 Steps to a Great Panel Discussion

    Type

    Blog Post

    Published

    Oct. 22, 2014, 1:57 p.m.

    ...things rolling. It can make or break the discussion section. And what about those audience members that want...

20. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type

    Blog Post

    Published

    June 5, 2014, 3:32 p.m.

    ...using biotechnology) were thrown on the table and dissected with equal enthusiasm. It may have started as ...