We narrowed to 598 results for: abo.1

...light-sensitive LOV2 domain of Avena Sativa phototropin 1 (AsLOV2). In the dark, this peptide is caged by the...iLID nano and 800nM to 47µM for iLID micro; Figure 1). With this great affinity range, these LIDs have ...Figure 2). They are all available now at Addgene. 1 Lungu et al., Chem Biol. 2012 Apr 20;19(4):507-17....Guntas et al., Proc Natl Acad Sci USA 2015 Jan 6; 112(1):112-7. doi: 10.1073/pnas. CRISPR-Cas9 optogenetic... al., BMC Molecular Biology. 2013 August 20th; 14(1). TRICK: A method for visualizing the first round...for a variety of experimental purposes including (1) single or multiple, constitutive or inducible, gene...the years. The authors optimized these systems by: 1) decreasing the number of plasmids required for each...

...by insertion and deletion frequency. Fig. 1: Overview of TIDE. The input is sequencing trace files...keep your product to knock-in size ratio below 10:1. Small knock-ins Is your knock-in edit small, with... your edit, it’s important to know a few things about your target cells and the desired edits. First, ...et al., NAR). It works just like TIDE (outlined above), except that it uses a 3rd sequencing trace file...great option, especially if the options outlined above aren’t realistic. Check Out Our Post on Sequencing...higher probability locations. If you’re worried about off-target effects, you can consider using a version...SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options in our blog post here. Dealing with...

...activity to a transcriptional activation domain (Fig 1). Two ways to use CRISPRa for your research CRISPRa... Stucka R, Hauber J, Lange UC (2016) Targeted HIV-1 Latency Reversal Using CRISPR/Cas9-Derived Transcriptional... organisms (Chavez et al., 2016). To learn more about the different types of Cas9 activators, check out...covering VP64, Suntag, SAM, and VPR. Worry less about off targets Unlike Cas9 cutting activity, off target...of the Addgene Scientific Advisory Board. His laboratory at Columbia University is focused advancing scientific... Variant for Your Next Experiment Find out More about Genome-wide Screens with CRISPR/Cas9 Learn How CRISPR...

...great advice on all of these topics. Listen to Part 1 Here Listen to Part 2 here! Time : Topic 0:00 - 0:...How did you hear about post grad opportunities and when did you start thinking about them? 18:30 - 19:... top if you leverage your community, think hard about picking a good mentor, and begin considering career...19:54 : How to think about picking a post-doc project 19:55 - 22:40 : Improvements that can be made to ...

...school to a startup and back to academia. Chapter 1: How working at a startup prepared Tom for an academic...synthetic yeast chromosome. Listen to learn more about Dr. Ellis, synthetic biology, synthetic chromosomes...Additional Resources from the Addgene Blog Learn about the Synthetic Biology Education Nonprofit BioBuilder...BioBuilder Plasmids 101: Golden Gate Cloning Learn About Working at a SynBio Startup in Our Interview with...

...Figure 1). Clustered events are called a population.                             Figure 1: While...series of one- or two-marker plots, like in Figure 3 above, until the population of interest has been successfully...  Of course, there are many more things to learn about when diving into flow cytometry data. There’s different... ways to use FSC and SSC, as briefly alluded to above. There’s many variations in how one can gate, select...

...single neurons in the living brain   Figure 1: Plasmid mix to label neuronal morphology (eYFP) and...genOway press release for more information. References 1. Atasoy, D., Y. Aponte, H. H. Su, and S. M. Sternson... on the Addgene Blog Learn More about FLEX-vectors Learn More about Cre-Lox Check out Retrograde AAVs...expression have been developed. Read on to learn more about FLP-FRT, Cre-lox, and how combinations of FLP and...

...folding, and deal with sub-optimal codon usage (Table 1) A few mutations are common to all or most expression...curated list of Bacterial Expression Systems Table 1: E. coli expression strains  Note: All strains are...How does inducible expression work? As mentioned above, many expression plasmids utilize inducible promoters...see protein overexpression? The strains described above should generate sufficient expression levels for...may help with solubility issues. We want to hear about your favorite protein expression tips and tricks...

...straight to your materials of interest!   Figure 1: The new search bar.   Pro tip! You can use a manuscript...straight from the new search bar, but the menu just above it can help you quickly and easily navigate to educational...down? You’ll find Blugene, as well as information about Addgene, our mission, links to our deposit, request...

...forward to another great year of sharing!   References 1. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9 knockout...cells are avaliable for distribution. To learn more about pooled library screening, read our recent blog post...the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some Common...

...older epitope tags - only time will tell! References 1. Georgieva, Maya V., et al. "Inntags: small self-structured...takes a lot of time and energy, Martí Aldea and collaborators have created a set of “innocuous tags” (inntags...Pubmed PMID: 23435261. Resources at Addgene Read about other Protein Tags in our Plasmids 101 Series. Get...

...PGC-1 plasmids from Addgene. References: Skeletal muscle PGC-1α1 modulates kynurenine metabolism and...Institute on Aging in a commentary about the new work published in Cell Metabolism. But, they note, there is plenty...diseases.” I don’t know about you, but I think I could use a good run right about now. And, if you are looking...be important to identify the key proteins and metabolites that are dynamically secreted by muscle during...

...an antibody performed in an experiment.   Figure 1: The front page of the Addgene Data Hub.   Antibody... antibody worked under the conditions described above, and a short description of how the antibody performed...

...sequence on either side of the desired mutation (usually 1-3 mismatched bases) is sufficient for your primers...researcher at Cornell University.       References 1. Breslauer, Kenneth J., et al. "Predicting DNA duplex... Brush up on Your Cloning Techniques Learn More about Plasmids in Our Plasmids 101 Series Use CRISPR to...

...is here to help. Contact us at [email protected] or +1 617-225-9000, or check out our MTA reference pages...Agreement (MTA) matters: Science thrives on collaboration (it’s the heart and soul of Addgene’s mission...scientists are sharing their materials. Questions about intellectual property rights, pending publications...

...concerned about possible unintended consequences of a march. Three of these risks include: 1) Lost trust...education, government, and civilization in general (1). Science and politics? Science is an apolitical process... with a testable hypothesis - an educated guess about how some part of the world functions. Experiments...governments exist in reality and science is how we learn about reality. Formally, a government can be defined ... More concretely, scientists gather information about reality informing people and their governments on...Democrats more often than Republicans, the March is not about Democrats or Republicans (16). This is a Pro-Science... have more weight than how any one person feels about it. And yet research shows that thoughts on some...

... learn more about Science communication check out these SciComm blog posts. References 1. Bourdeau, Raymond...When you think about going to a scientific conference, you may think about sitting amongst a sea of chairs...After learning about using sound to visualize bacteria in the lab, I was excited to learn about creative approaches... at different ultrasound frequencies. Read more about ‘Using ultrasound to image bacteria in vivo: acoustic...Protein Resources and get started!  I learned a lot about creative ways to do science communication and outreach... Methods for Bacterial Genome Engineering Learn about Science Communication using Twitter  Resources ...

...may contribute to more in the future. Figure 1: A timeline of CRISPR base editing advances that led...Lander, E. S. (2016). The Heroes of CRISPR. Cell, 164(1), 18–28. https://doi.org/10.1016/j.cell.2015.12.041...Baby KJ” was diagnosed with a rare and dangerous metabolic disease shortly after his birth. Within a seven-month...successful base editing intervention was created. Only about half of babies with KJ’s disorder survive long enough...effort was only possible because of a massive collaboration between research hospitals, universities, private...an astounding feat of public investment and collaborative scientific effort. As we like to say at Addgene...activity of adenine base editors The first ABE was about 50% efficient in human cells on average, but its...

...compare the experimental group.     Figure 1: A, Most biological experiments will benefit from ... Histochemistry & Cytochemistry, vol. 59, no. 1, SAGE Publications, Jan. 2011, pp. 6–12. Crossref,...subjected to the experimental condition. In the example above, a negative control could be a third group of mice...in the experimental protocol. In the experiment above, you could repeat the tau western blot several times...Baseline secondary signaling levels In addition to the above-mentioned safeguards, you’ll need to test the secondary...Flow cytometry In addition to the controls listed above, viability and compensation controls are recommended...technique before starting. For more information about antibodies and antibody-based experiments, check...

...in the crystal jellyfish Aequorea victoria (Figure 1) in molecular detail. On the underside of the “umbrella...ways scientists have adapted them for use in the laboratory. The discovery of GFP The story of how fluorescent...particularly interesting. Although humans have known about bioluminescent organisms and their characteristic...sequence. Soon after that, in 1994, Prasher’s collaborator Martin Chalfie expressed GFP in exogenous organisms...studying it grew. In particular, studies in the laboratory of UCSD biochemist Roger Tsien resulted in much...for you, so if you’re interested in learning more about fluorescent proteins, I invite you to download our...