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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 609 results for: cas

Showing: 361 - 380 of 609 results

  1. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...should be interesting; however, this should (in most cases) not be the driving factor. The following questions...head for a career in pharma or biotech, this is one case where the topic of your research can make your transition...

  2. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...up positive traits (i.e. the plasmid contains a cassette that will make cells resistant to a toxin), whereas... a negative trait (i.e. the plasmid contains a cassette that will make cells sensitive to a toxin). In...

  3. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...validate your antibody on crosslinked proteins. In the case of histone modifying proteins, which bind quite ...abundance transcription factor or cofactor. In such cases, the gentler enzymatic digestion can preserve more...

  5. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...following exposure to a brief heat shock. In the case of Hsp70, the heat shock releases heat shock factor...depositors have developed heat shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans...

  6. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...photons or odorants, to elicit downstream signaling cascades.  So let’s break down the name "G-protein coupled...then induce or inhibit intracellular signaling cascades as a response to the extracellular stimuli. Ligand...

  7. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...during in vivo research and the CARE guidelines for case reports are two sets of guidelines frequently used...manuscript is accepted for publication and is, in most cases, blind (meaning you don’t know who the reviewers...

  8. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...primary-conjugated) or indirect (secondary) methods. In those cases, remember that a secondary should be used when signal...you still have a number of options. If this is the case, you can narrow them down in a variety of ways. ...

  10. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...intensity remains. The first issue is that, in several cases, the fluorescence intensity decay of a fluorophore... the excitation power (Grünwald et al, 2008) occasionally and certainly after a set-up has been changed...

  11. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...given threshold within the cell, as is often the case upon viral infection. Endogenously produced dsRNA...the Addgene blog CRISPR 101: RNA Editing with Cas13  RNA Interference in Plant Biology  ...

  12. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...oligonucleotides specifying a gRNA target sequence into a Cas9-containing plasmid such as pX330. This cloning strategy...are using the Golden Gate method to create CRISPR/Cas9 constructs, assemble standard plasmids parts in ...

  14. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...large (1-10) and small (11) fragments. In several cases an FP(1-10) fragment may be compatible with multiple...Split-horseradish peroxidase (Alice Ting Lab 2016)  Split-Cas9 for inducible genome editing (Feng Zhang Lab 2015...

  15. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...conjugated substrate is added to the wells. In the case where the detection antibody is biotin-conjugated... protein within a mixed sample. However, in this case your protein of interest is pre-incubated with a...

  17. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...the heading "Addgene Partial NGS Result." In some cases, there will be more information about our sequencing..., Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

  19. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ... process, following sequencing and assembly. In cases where the QC team suspects an assembly issue, they...and be made available for distribution. In these cases, the QC team leaves a note on the plasmid page to...

  20. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ... Let’s get started! Before you begin In the best case, you know ahead of time that you want to probe your... to lose by trying to strip and reprobe. In this case, we recommend using mild stripping solution (see...
Showing: 361 - 380 of 609 results