We narrowed to 821 results for: nin

...antibody-producing B cells that cannot replicate, meaning that only hybridomas will survive this step. Then...antibodies will exhibit off-target binding to unintended proteins. This is because monoclonal antibodies...then used to produce all lots of the antibody, meaning that the antibodies are largely identical between...antibody that is actually produced by the hybridoma, meaning that there will be changes between lots over time...

... translation (Noren et al., 1989). In the late nineties, this was developed into an E. coli system, with...will be needed to introduce the gene of interest containing the chosen codon that will result in the incorporation... parameters the same for all three components, meaning that the final protocol may involve a sensitive... located at Oregon State University focused on turning GCE tools developed in the literature into robust...

...introduce frameshift mutations in genes of interest, turning expression off permanently. Viral vectors can ... preserving cell viability. Library delivery Screening libraries, large collections of biological independent...library cargo delivered are controllable. For many screening applications, it is essential to deliver a single...Gabuzda D, Crowe SM, Mills J, Lewin SR, Brew BJ, Cunningham AL, Churchill MJ. Pathogenicity and immunogenicity...

...time-lapse images, quantitatively evaluating antibody staining, or counting cells are now routine, open-source...community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation of ...via globally established makerspace networks and training users via workshops, seminars, or hackathons greatly...research. The EU, for example, is calling for the opening of sources within the next few years, with the ...

...drastically affects their stable folding and efficient functioning. But what about placing the mesophilic hosts ...consider them worth the trouble.  Enter ELSA: E. coli Learning Suboptimal Acclimatisation To resolve this impasse...coli! Additionally, we’re also working towards widening the applicability of our strategy using the pMBP-LbCas12a...conventional academic settings, these are an eye-opening experience for cultivating a nuanced understanding...

...involves two basic components: the pigments (anthocyanins), and the plant circadian clock. Petunias have...the biochemical pathways involved in central anthocyanin metabolism are well understood. Additional work...comprehensive understanding of the genetic pieces underpinning these mechanisms – our project wouldn’t be possible...biology. There are already two comprehensive plant cloning kits available in the repository, MoClo and GreenGate...

..., David Liu’s lab developed split cytosine and adenine base editors that can be reconstituted in vivo ...overlap, Yiyang Gong’s lab created two plasmids containing a red-shifted Ace-mScarlet, a fusion of a voltage-sensitive...via FRET. Image from Beck and Gong, 2019. Broadening the spectral diversity of GEVIs can enable resolving...Article contributed by Andrew Hempstead The Modular Cloning (MoClo) system allows synthetic biologists to use...

...which Susan replied, “What’s a science writer?” Learning about SciComm by doing SciComm Writing had always... terms of PR types of things or communications planning.” To beef up her resume, she decided to complete...mind process things. Sometimes finding that uninterrupted time to be creative is challenging,” says Susan...Susan  finds important for her work include: “Listening. Evaluating evidence. Critical thinking. Being...

...other antibodies in the experiment. If you are planning to use multiple antibodies in a single experiment...independent antibody strategies that compare the staining patterns of multiple antibodies against the same...express a tagged version of the antigen and compare staining of the antibody to that of the anti-tag antibody...negative controls as you get your assays up and running.  Ed.'s note: More questions on antibody validation...

...permanently or temporarily, we’ve got you covered. Defining your control requirements To pick the best tool...permanent control mechanism? Where is the control happening? Is the regulation of an exogenously introduced... Turn it on forever Permanently increasing or turning on expression of a new protein is a cinch. Stably...et al. Check out Addgene's Lentiviral dTAG cloning vector and SMASh degron tagging vector! Destabilizing...

...withdrawn from the US market because of life-threatening health effects. Eight of these posed greater ... important to get the research right from the beginning. This is the goal of Gendered Innovations. This...biological variable in all NIH-funded research. Janine Clayton, Director of the Office of Research on ...compared to that of human clinical trials. And, as Janine Clayton points out, late-stage clinical studies...

...photoconvertible variants, it seems like GFP is always learning new things. Now, work from Connie Cepko’s lab ...as a scaffold suddenly seemed very realistic. Envisioning GFP as a substrate promoting dimerization, Tang...channelrhodopsin-2, commonly used in optogenetics, opening up the possibility of adapting GFP lines for optogenetics...Listen to Our Podcast Interview with Connie Cepko Combining Cre and GFP with Cre-DOGs The T-DDOG results showed...

...figure out how to optimize protocols for in vivo screening with lentiviral pooled shRNA libraries. Later,...When using the ClonTracer system, lentiviruses containing these barcodes infect the target cell population...infected a non-small cell lung cancer cell line containing an activating mutation in epidermal growth factor... Blog Learn about the Sabatini and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource...

...transition out of the academic sphere into a different meaningful career in science. Supervisors in the academic...scientific backgrounds. For a stated purpose of transitioning a scientist from academia to industry, you might...although most scientist coaches can help with transitioning out of academia (because so many scientists ...Website Check out Our Career Page for Recent Job Openings Find Plasmids to Expedite Your Research   ...

...single sentence and figure must be clear, concise, meaningful, and supportive of the mission of the application...of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to the sentence. Below are...alternative solutions to demonstrate thorough thought planning on the matter. The more detail (within the page...Application" by Vid Mohan-Ram in Science Careers "Beginnings - How to write your first grant proposal" by ...

... allows certain unintended edits to escape cellular surveillance. To limit unintended incorporation of...important to test different lengths and sequences, as unintended secondary structures in your pegRNA could inhibit...3′ end to protect them from degradation. When designing epegRNAs with large structured motifs like mpknot...

...assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded... is sufficient to disrupt dimerization while maintaining the bright and stable fluorescence of the original...also named their variant mStayGold. It is worth mentioning that Kiryl Piatkevich and colleagues generated... Figure 3: Overview of library generation and screening that led to identification and characterization...

... calcium binding proteins (e.g., calmodulin or troponin C). Their fluorescent readouts are used to monitor...can be delivered to intact tissues and organs noninvasively and target a defined population of cells (e....to endogenous calmodulin. Multiplexing Are you combining your calcium reading with other measurements, ... is a FRET-based calcium reporter derived from troponin C. It is engineered with a reduced number of calcium...

...Gate Assembly for cloning, you might be familiar with the rules of thumb for designing your overhang sets...Additionally, this assembly, which used only antibiotic screening for selection, failed under normal cycling protocols...complexity allowable by a favored, fairly easy cloning technique (which may be of particular interest ...

... genome, the virus is replication incompetent, meaning it can’t replicate and continue the infection cycle...known to cause respiratory diseases in humans, earning this virus a BSL-2 classification. Lentivirus (... can be used for extra security. Finally, when spinning down lentivirus, you’ll need to use centrifuge...trained in each new place (and pay attention to the trainings!), even if you know guidelines for best practices...