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  1. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...acid purification methods not mentioned in today’s post? Let us know in the comments! Additional resources...protocols and tips Browse our plasmid cloning blog posts Resources on Addgene.org Find protocols for molecular...
  2. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ... blog posts related to viral vector protocols and tips Read all of our viral vectors blog posts Resources...
  3. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ... Thisted, Thomas, and Kenn Gerdes. "Mechanism of post-segregational killing by the hok/sok system of plasmid...to efficient cloning Read all Plasmids 101 blog posts Read about knockout/knock-in plasmids Resources...
  4. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...experiment, they definitely made my life easier!  In this post, I’ll share some AAV production, purification, and...account for batch-to-batch variability. Check out this post to learn more about different ways to titer AAV....
  5. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...Additional Resources on the Addgene Blog Read this blog post about CRISPR Kinome Libraries available at Addgene...for large-scale functional screens Read this blog post about genome-wide screening using CRISPR Resources...
  6. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...This post was contributed by the Mesothelioma Cancer Alliance. We have learned much about the causes ...be protected from this dangerous material. This post was contributed by the Mesothelioma Cancer Alliance...
  7. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...This post was contributed by guest blogger, Luke Lavis, a Group Leader at the Janelia Research Campus... blogger, Luke D. Lavis! All images in this blog post were contributed by Luke D. Lavis. Luke D. Lavis...
  8. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ... This post was contributed by Jo Miller, founder and principal of J. Miller & Associates, Inc., a national...Grant_Chat    Additional Resources Read other Career Posts on the Addgene Blog! ...
  9. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ... list more common tags than are described in the post, but have been categorized to help you better assess...Healthcare Life Sciences. Addgene pages and blog posts of interest: Which Fluorescent Protein Should I...
  10. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...viral vector services and resources!   This blog post was written by Brook Pyhtila and Brian O'Neill.  ...
  11. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...sites! In this post, we'll cover Cas9 multiplexing options, but also check out our blog post about multiplexing... information on this system, check out this blog post.  Multiplexing in zebrafish Wenbiao Chen Lab Golden...
  12. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...having issues, you’re in the right place! In this post, we’ll talk about western blot optimization and ... on your gel or blot, Table 1 (at the end of the post) has a number of helpful suggestions.  Gels Gel ... and 2 in our Technical Design of a Western Blot post to make sure you have the optimal gel for your experiment...consider (see our “technical design of a western blot”) post, most imaging issues can be fixed real-time by exposing... Splice variants Check literature Posttranslational modifications Check literature Highly...
Showing: 361 - 380 of 706 results