We narrowed to 695 results for: prot

...label neuronal morphology (eYFP) and the synaptic protein PSD95 (PSD95-mcherry) under the control of Cre ...fluorescent cytoplasmic markers (e.g. eYFP) and synaptic proteins (e.g. postsynaptic PSD95-mcherry) are used in ...In order to analyze the function of individual proteins in neuronal circuits or synaptic remodeling, researchers...be induced by Cre recombinase expression while proteins needed for later experiments (cellular tracking...is fascinated by the question of how genes and proteins coordinate billions of neurons to form the functional...

...elegans. Other groups have used Mango to study RNA-protein complexes (Panchapakesan et al., 2017) and short...selection of an RNA mimic of green fluorescent protein by fluorescence-based selection and directed evolution... Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein purification and characterization using RNA...highly efficient RNA mimics of green fluorescent protein." Nature structural & molecular biology 21.8 (2014... Resources at Addgene.org Browse fluorescent protein plasmids and resources See all Jaffrey lab plasmids...

...reporter constructs), in functional analysis of proteins (to perform alanine scanning mutagenesis or targeted...using primers (with the desired mutation) in a PCR protocol that amplifies the entire plasmid template. The...simply following the polymerase manufacturers’ PCR protocol. Be sure to adjust the extension time to match...sites. Alternatively, if you want to make your protein non-functional, you may simply introduce a frame-shift... on Addgene.org Browse Our Molecular Biology Protocols Find Empty Vectors for Your Next Cloning Experiment...

...detection because they produce very little or no viral protein. To solve this problem, two strategies have been... HIV so that infected cells will produce viral proteins and die, either through cytotoxicity or the immune...expression. In cell lines that can produce HIV-1 toxic proteins, CRISPR/Cas9 SAM caused apoptosis, indicating ...However, this approach could permit some viral protein production, so researchers are also interested ...antiviral therapies target HIV-1 at the level of protein structure, CRISPR gRNAs require DNA sequence-specific...

...Plasmid Cloning Protocols Find Virus Protocols Find Basic Molecular Biology Protocols Gibson Assembly...can be mixed yourself (e.g. see the Miller Lab Protocol). The Gibson assembly process can be used to assemble...commitments. These kits also come with specific protocols, suggestions for ratios of product to insert, ...

...uses the direct injection of a CRISPR/Cas9 ribonucleoprotein complex with linear DNA as repair templates...development of CRISPR/Cas9 editing methods, other Cas proteins have been adapted for use in C. elegans. In 2017... mammalian and yeast cells using modified Cas9 proteins to regulate gene expression (CRISPRi/CRISPRa),... genomic loci (CRISPR-imaging), or to find the proteins associated with a given genomic locus (CRISPR-...improve genome editing and conditionally manipulate proteins. Learn more at his webpage or follow him on Twitter...

...in a direct or unbiased way at the DNA, RNA, or protein level. The “correct” choice of tool requires careful...unmatched—unlike CRISPR experiments that require exogenous protein, successful induction of RNAi only requires the...scored. However, determining the transcript or protein abundance of your specific target required physical...possible in plants outside of limited, single cell protoplast analysis. Instead, microscopic plants must be...Bush J, McCormack M, Sheen J (2013) Comprehensive Protein-Based Artificial MicroRNA Screens for Effective...

... in skeletal muscle by exercise and endurance, protects the brain from depression. Mice that are genetically...stressors as usual, but somehow their brains were protected. Effects of Activation of the PGC-1a1 Pathway ...levels of kynurenine in the body, the brain is protected from stress-induced biochemical changes that could...explore. “It will be important to identify the key proteins and metabolites that are dynamically secreted ...

...encodes for a small Cas protein (40-70 kDa), which is half the size of other Cas proteins found in the so-called...adaptive immune systems that provide bacteria protection against infectious agents. Several enzymes behind... of the larger and more complex Cas9 and Cas12 proteins. Cas14 cleaves ssDNA But what is the function ...

...in government, evidence to inform policies, a protected place to work, funding for science, and numerous...ultimately galvanized scientists to rally in Ottawa to protest the “death of evidence,” and to speak out in unprecedented...water. Many marchers held signs demanding the protection of our Great Lakes and our planet. And as we ...Nashville, TN, USA). I had never participated in a protest before, so I had no idea what to expect. Admittedly...although there was at least one person vocally protesting us, he was not threatening in any way. Participating...

...Your Fluorescent Proteins for Multi-Color Imaging Choose the Brightest Fluorescent Protein for Your Experiments...Addgene.org Browse Our Fluorescent Protein Resources Find Empty Fluorescent Protein Backbones Find Plasmids for... fluorescent proteins...

...Technique: Probe Phage Genomes for Host Binding Proteins & Running for Rare Disease, Running for FOP, Running...Technique: Probe Phage Genomes for Host Binding Proteins," was contributed by guest blogger Jessica Sacher...describes a technique you can use to find phage proteins that bind to your bacterium of interest. For those...an ingenious but simple technique to screen for proteins that could be useful for a wide variety of reasons...

...Davidson Fluorescent Protein collection, a ‘One-Stop Shop’ for Fluorescent Proteins, and plasmids from ...for tagged and untagged expression of SARS-CoV-2 proteins in bacteria, yeast, and mammalian cells. We teamed... determine the three dimensional structures of proteins.  The RESOLUTE Consortium collection enables research...

...include enrichment against kinases, cell cycle proteins, and more.  “In the past, genome engineering with...leveraged the CRISPR system to target all 19,150 mouse protein coding regions with 87,897 unique gRNAs. Their ...aid efforts to understand the function of both protein-coding genes as well as non-coding genetic elements...might need larger numbers.” Further details and protocols are available online on Addgene's CRISPR Library...

...an affiliated TniQ protein recruits transposition cofactors. The transposon proteins integrate a payload... combines with a donor template and transposon proteins (Tns) to integrate large sequences into genomic... using CRISPR-associated transposases. Nature Protocols, 19(3), 752–790. https://doi.org/10.1038/s41596...

...advantage of bacterial characteristics such as prototrophy, the ability to synthetize all compounds needed...synthesize a compound needed to grow. Auxotrophy and prototrophy come into play during cloning if you’ve designed...example, Markus Ralser’s lab created a yeast prototrophy kit containing 23 plasmids to complement auxotrophy...molecular biology reference Watch molecular biology protocol videos ...

...Science Resources on Addgene.org Find Protocols and Protocol Videos Check out LeGO plasmids from the... very human side to scientific research. Find Protocols Lab Tips to Keep Your Experiments Running Smoothly...this world where we are often trained to follow protocols, guidelines, and standards. Bricking science reminded...

...guest blogger Meghan Monroy, a graduate student in Protein Science at the University of Connecticut. Molecular...at the University of Connecticut interested in protein science.     References: 1. Celie, Patrick HN, ... Perrakis. "Recombinant cloning strategies for protein expression." Current Opinion in Structural Biology...Empty Vectors for Your Research Find Additional Protocols Browse Our Special Collections for Plasmids from...

...aminoglycoside Binds 30S ribosomal subunit; interrupts protein synthesis Bactericidal 7.5-50 ug/mL Streptomycin...Streptomycin aminoglycoside Inhibits initiation of protein synthesis Bactericidal 25-100 ug/mL Ampicillin...tetracyclin Binds 30S ribosomal subunit; inhibits protein synthesis (elongation step) Bacteriostatic 10...Molecular Biology Reference Pages View our Plasmid Protocols Find Your Perfect Plasmid Backbone ...

...libraries In surface display, proteins of interest are fused to anchoring proteins to be expressed on the surface...systematically explore variations in the spike protein, teaching us more about viral evolution and antibody... Pooled Libraries CRISPR Library Amplification Protocol ...