We narrowed to 521 results for: she
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TypeBlog PostPublishedMay 21, 2020, 1:15 p.m....Isaacman-Beck J, Paik KC, Wienecke CFR, Yang HH, Fisher YE, Wang IE, Ishida IG, Maimon G, Wilson RI, Clandinin...
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Plasmids 101: What is a plasmid?
TypeBlog PostPublishedApril 2, 2020, 6:17 p.m....Originally published Jan. 14, 2014 and updated Apr. 14, 2020. Any newcomer who joins a molecular biology... -
Year of the Camelid: Antibody Style
TypeBlog PostPublishedMay 9, 2024, 1:15 p.m....antibodies and affinity reagents. But for a quick refresher, some of the advantages of sdAbs over conventional... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog PostPublishedDec. 3, 2020, 2:15 p.m...., and VP3, which together form the outer capsid shell that protects the viral genome. The cap gene is ... -
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene
TypeBlog PostPublishedJuly 2, 2015, 8:37 p.m.... Just over a month ago I finished up my PhD in Biological and Biomedical Sciences at Harvard University... -
Management for Scientists: Giving Feedback
TypeBlog PostPublishedFeb. 6, 2015, 2:46 p.m....Addgene Blog to follow the entire series as it is published. For more reading try The Discipline of Teams ... -
Management for Scientists: What Makes a Good Manager Anyway?
TypeBlog PostPublishedDec. 9, 2014, 2:22 p.m....Addgene Blog to follow the entire series as it is published. Good introductory reference books on managing... -
Choosing Your Perfect Empty Backbone
TypeBlog PostPublishedAug. 19, 2014, 3:39 p.m....backbones that have been designed, tested, and published by academic scientists. To help you find the vector... -
Advice for Choosing a Research Project
TypeBlog PostPublishedApril 17, 2014, 2:45 p.m....community. In the long term, the research project establishes your professional brand. It offers colleagues... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog PostPublishedMarch 9, 2017, 3:40 p.m...., interests and passions is another great way to shed light on what paths to pursue. Spend some time really... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog PostPublishedJan. 25, 2018, 2:09 p.m....well-controlled. The lack of site selectivity typically diminishes the apparent affinity for the ligand, especially... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....using sgRNAiBAR. Image from Zhu et al., 2019. Wensheng Wei's lab at Peking University has developed a... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog PostPublishedMarch 5, 2020, 2:15 p.m....which is equally important to report. We recently published a paper in Frontiers in Neuroanatomy and summarized... -
Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog PostPublishedJan. 22, 2019, 2:41 p.m....oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy Resources on Addgene.org... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog PostPublishedMarch 28, 2022, 4:30 p.m....Bandrowski et al., 2016). It’s also been well established that recombinant antibodies are excellent for... -
Antibodies 101: Chimeric Antibodies
TypeBlog PostPublishedApril 25, 2023, 1:15 p.m....Protein B. The antibodies are both extensively published, knockout validated, and work in your application... -
What the HEK?
TypeBlog PostPublishedApril 18, 2023, 1:15 p.m....it is most likely adrenal in origin. (Anatomy refresher: the adrenal gland is next to the kidney and is... -
Plasmids 101: Origin of Replication
TypeBlog PostPublishedNov. 11, 2020, 2:23 p.m....Originally published Feb 6, 2014 and last updated Nov 10, 2020. Now that we know all about antibiotic... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....nts, and is thermostable between 31-100°C. They published their finding in a bioRxiv article and in PNAS... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog PostPublishedJune 9, 2016, 2:30 p.m....Impressions Here References 1. Gao, Feng, Xiao Z. Shen, Feng Jiang, Yongqiang Wu & Chunyu Han. “DNA-guided...