Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 640 results for: son

Showing: 361 - 380 of 640 results

  1. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ... Quick tips for writing a good cover letter Personalize your cover letter to the job and job requirements...Soriano-Carot in September 2016 and was updated by Rachel Leeson in May 2024.     Additional Resources on the Addgene...

  2. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ... and for a very brief period of time, the only, person to know a brand new fact is often enough to keep...learning to “speak science,” and virtually every person you talked to during the day was also a scientist...

  3. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...becoming an expert in your field and are the perfect person to be writing the review! Doing so is a great way...Good luck! References 1. Haery, Leila, Ryan C. Thompson, and Thomas D. Gilmore. "Histone acetyltransferases...

  4. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...of false interpretation in cell culture for this reason. Unreproducible results are rife. Misidentified...PMID: 12001993. 4. Lucey, Brendan P., Walter A. Nelson-Rees, and Grover M. Hutchins. "Henrietta Lacks,...

  5. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...consider using moxFPs over standard FPs for the reasons listed above. In addition, because the moxFPs are...11736655. Pubmed Central PMCID: PMC1222268. 7. Aronson, Deborah E., Lindsey M. Costantini, and Erik L....

  6. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...summarized in a short blog post, but some examples I’m personally interested in include: intracranial self-stimulation... to proceed with your behavioral experiments. Comparison of c-fos amongst animals that receive different...

  7. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...act on DNA, or are enzymatically active. For this reason, recombinant proteins are typically expressed in...culture from reaching the desired density within a reasonable time-frame. For these cases, some strains carry...

  8. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...detection ranges of multiple targets. Convenient! Comparison of loading control approaches Cartoons are nice...all, but we’ll leave you with this real-world comparison of different loading control approaches from ...

  9. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...arguably the most widely and productively used  (Thomason et al., 2014). In recent years, CRISPR-Cas9 technology...reports 5 (2015): 15096. PubMed PMID: 28060411. Thomason, Lynn C., et al. "Recombineering: genetic engineering...

  11. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...Site-Specific Genome Alterations. Edited by Doudna J and Sontheimer EJ. Methods in Enzymology Vol. 546; (2015). PubMed...inactive CRISPR-Cas9 using asymmetric donor DNA. Richardson CD, et al. Nat Biotech. 34(3); (2016). PubMed...

  12. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...’s software in order to generate signals in a reasonable range. If colleagues in your group already have...interest can still be gated out later. Based on personal preference, some people like to make stricter ...

  13. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...do aptamers! Corn was recently described in 2017 (Song et al., 2017). Corn naturally dimerizes, and this...PMID: 28747322. PubMed Central PMCID: PMC5602116. Song, Wenjiao, et al. "Imaging RNA polymerase III transcription...

  14. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...is also being realized. There are a variety of reasons to consider using genome editing to change the ...yellow striate mosaic virus (Gao et al., 2019) or sonchus yellow net rhabdovirus (Ma et al., 2020) have already...

  15. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...RNAs." PloS one 11.2 (2016): e0149483. Rogers, Jameson K., and George M. Church. "Genetically encoded ...-tagged human ORF clones to allow testing and comparison of different cell-free expression systems; the...

  16. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...structure associated to these tools, skills, and personnel Define pricing according to all of the needed ...managers, and techs’ financial books.  To find personnel salary ranges, go to online job information sites...

  18. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...Sleeper, M. M., Sanmiguel, J., Wu, D., Gao, G., Wilson, J. M., & Sweeney, H. L. (2008). Adeno-Associated...Feng, Y., Tabar, M. S., Metierre, C., Parsania, C., Song, X., Wang, G., Su, X.-D., Bailey, C. G., & Rasko...

  19. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ... J. H., Kim, Y. S., Machado, T. A., Quirin, S., Benson, B., Kadmon, J., Raja, C., Chibukhchyan, A., Ramakrishnan... Jason Nasse...

  20. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...fluor through a process known as bioluminescent resonance energy transfer (BRET). The fluor then emits light...calcium release have been made using fluorescent resonance energy transfer (FRET) probes. These probes generally...
Showing: 361 - 380 of 640 results