We narrowed to 842 results for: Eras

...Future of rAAV gene therapy: Platform for RNAi, Gene Editing and Beyond." Human Gene Therapy ja (2017). PubMed...transduce many tissue types, it has low expression in several brain regions where other serotypes may perform... not desirable? If diffuse gene expression is tolerated, then axonal transport is fine and a researcher...not take much time at all. Much of the previous literature using AAVs for DREADD delivery indicates ~7-21...potentially toxic and would result in cell death. With several new constructs becoming available, AAVs could be...effects of metabotropic and ionotropic influences on overall cell function. AAVs are the most appropriate vehicle...being made to make them even more useful for therapeutic purposes. The University of Pennsylvania and ...

... blog Management for ScientistsTransferable Skills Guide: LeadershipTransferable Skills Guide: Teamwork...the right time. Like many of the posts in our Transferable Skills series, this post is not meant to be ... that you could strengthen. The right set of transferable skills, coupled with your technical skills, ...support each team “owns” is critical for day-to-day operations and keeping our customers happy, but inevitably...at that given time. I think about how previous iterations of those teams resolved similar issues in the...based on various levels of assertiveness and cooperation: avoiding, competing, accommodating, collaborating...

...feel they lack formal training in a variety of transferable skills. At Addgene, we've set out to fill this... highlighting that researchers do learn MANY transferable skills while working in the lab and by offering...where you might need some help. Today in our transferable skills guide: Problem-Solving. I don’t know ... careers, I always felt that descriptions of transferable skills seemed fuzzy or ill-defined. In this ...because it helps me see problem solving as a generalizable process.  Step One: Identify the problem ...research to better understand it and come up with several hypotheses on the cause. To test the most likely...your life. This tactic is a good way to see how transferable these skills really are, while also practicing...

...slightly to Taq polymerase’s ideal temperature (70-75oC). At this temperature Taq polymerase can synthesize...reagents and several heating and cooling steps. PCR relies on heat resistant DNA polymerase from the thermophilic...Taq). Taq polymerase is thus a heat resistant enzyme that can withstand changes in temperature. Taq was...this temperature the primers can anneal to the single stranded DNA template strands. DNA polymerase is ...also stable at this temperature and can bind to the primers.  Extension- The temperature is then raised slightly..., or for generating mutated sequences for site-directed mutagenesis.  Quantitative polymerase chain reaction...What is polymerase chain reaction? If you have ever worked in a molecular biology laboratory you have...

...Page Read Other Posts in the Transferable Skills Guide Developing Transferable Skills During Science Training...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this... highlighting that researchers do learn MANY transferable skills while working in the lab and by offering...where you might need some help. Today in our transferable skills guide: Collaborating with others outside... Addgene as a nonprofit organization is very considerate and thoughtful about its limited resources. Rather... will often look for partnerships where we can leverage another organization’s expertise to our mutual... other projects like it going, someone (likely several people at Addgene) had to take the initiative to...

...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this... highlighting that researchers do learn MANY transferable skills while working in the lab and by offering...where you might need some help. Today in our transferable skills guide: managing a team. You’ve just been... transferable skills...

...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this... highlighting that researchers do learn MANY transferable skills while working in the lab and by offering...where you might need some help. Today in our transferable skills guide: Time Mangement for Scientists.... Don’t be afraid to accomplish some quick but moderately useful tasks at the expense of time spent on ...appropriately. Back in my undergrad days, I had general goals for my experiments, but didn’t spend enough...achieve these goals. I have far too many projects to operate this way now. Talking about “planning” is easy,... arises.Why do I write things down? It’s more viscerally appealing for me to physically cross things out...

...s post, we’ll discuss some of the important considerations you should think about when developing optogenetics...place aversion also models reinforcement but is considerably simpler than ICSS. The test chamber is divided...capture software such as EthoVision. Consult the literature to find all the applications of optogenetics ... of the type of behavior, certain technical considerations still apply. Testing stimulation First, In ...started with a brain region that was known to be generally associated with a specific behavior but that they...mouse; however, all the steps and technical considerations are directly applicable to the rat [6]. Technical...

...within and outside your field, and any number of interactions with professionals from other backgrounds at...simple, but they could in fact be interpreted in several different ways depending on the team. When Tony... from completing 2-3 large projects a year, on average, to completing 8-10 projects per year. Happier ...resources on the Addgene blog Read about other transferable skills Find all of our science career related...

...representative images, measuring the main outcomes in several different ways, and other questionable research... tested with a new experiment; and hypothesis generation, in which data are investigated to look for unexpected...trends or differences between groups. Hypothesis generation is critical for advancement, but we are too often...using hypothesis testing statistical tools that generate a p-value.  As an example, if you hypothesize ...clear distinction between hypothesis testing and generation, can be sure that results will be reported regardless...