We narrowed to 277 results for: ATC

...least a year when stored at -80oC.   For each new batch of competent cells, don’t forget to do a negative...the number of colonies obtained using your fresh batch of competent cells with those obtained using commercially...commercially available cells or previous batches of homemade competent cells to ensure good efficiency....

...optimized until the multiplexed and individual values match across the board. If the samples or sample conditions...need to make sure the assumptions in your dataset match the assumptions in your analysis method. ΔΔCT/CQ...’re not sure if your amplification efficiencies match, you’ll need to run a validation assay in which ...favorite (...er, whichever one uses assumptions that match your data set, that is.) Yuan, 2006 has a good overview...

...team in the UK is five hours ahead, so I’m always catching up in the morning! 9:00 AM--Technical implementation... team to discuss automated protocols. 12:00 PM--Catch up. Finish any communications with my UK team members...out. 4:00 PM--Respond to customer help tickets. Catch up on customer help tickets, and respond when I ...

...working with Addgene since 2014 so I’ve been able to watch the growth since then. What do you think is the ....Paratore: I’ve certainly had a front row seat watching the international growth through my work on the...have two children active in sports, so I enjoy watching their games.  If I’m not at game, I’m either out...

...networker (or if you just aren’t doing it at all yet) watch my webinar, “Not Networking 101-Building Relationships... the country with groups who are piloting this. Watch for my upcoming mentoring post in which I describe...naturally out of friendships or work interactions. Watch for the people that are already teaching you and...

... “copies.” This homogeneity circumvents the batch-to-batch variability found in polyclonal antibodies,...to non-target proteins), variability (separate batches can perform differently – happens when the antibody...

...through the QC pipeline to resolve any issues.  Catching and addressing QC issues Addgene’s QC team has...discrepancies can be caught prior to distribution. Catching these errors prevents future experimental issues...shifts, missing elements, and nucleotide indels or mismatches in significant plasmid elements. Which differences...

...to share or remix the songs, the CDs could get scratched over time, and it was difficult to keep track ..., the long-term stability, consistency between batches, and molecular definition of recombinant antibodies...Antibodies blog post Learn more about hybridomas Watch our Antibodies 101: What is an Antibody? animation...

...activity on some mismatch-containing pairings. During Golden Gate assembly, ligation of mismatched pairs of overhangs...sequence-dependent ligation efficiency and identification of mismatch-prone pairings. Using these 4 bp overhang observations...

...still virgins. Females begin mating ~6 hours after hatching into their adult phase. It is important to clear...before the progeny begin eclosion, the process of hatching from their pupal case (~9 days after setting up...genetic cross). You can tell when pupae are ready to hatch the next day because the pupae will develop a visible...

...designed to have a perfect match to the reference genome – even single mismatches within a CRISPR guide can...for in the CRISPR guides they used, leading to mismatches between the guides and the lines’ genomes, and...

...does not require a PFS, and it is sensitive to mismatches in target RNA from bases 12-26 of a 30 nt target...edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-ADAR2DD(E488Q) displayed... targeted nucleotide. Introduction of guanine-mismatches in the guide across from these off-target edits...

...in the previous layout, the number of plasmids matching each filter is displayed in parentheses.  In this...available filters to refine your search results to match your experimental needs. Remember, only filters ...insert, or use the other options to find plasmids matching your search terms with gene and guide RNA / shRNA...

...detail-oriented lab team that works carefully to catch mistakes. Each sample that is transformed is plated...scanned into our LIMS system to ensure that they match, helping us to avoid mixing up samples as we transform...process, we ensure that the sample tubes that we use match those of the appropriate deposit. Using barcoded...

...not function properly. This is the result of a mismatch between human and E. coli codon preference. Some... abundances of the codons I choose for the host match the abundances of codons used in the native organism... constructs. However, due to problems such as mismatched translation rates and potential effects on cell...

...sequence of interest (CDS1, top). Overhangs must match the Level 0 acceptor (bottom) for CDS1 parts. (B...four-base overhangs flanking each part, as they need to match sequentially in order to properly ligate. PRO, promoter...create the resulting plasmid map and identify any mismatches that are preventing your overhangs from aligning...

...Videos Over at the Addgene Youtube Channel   After watching these trends for a few years, I decided to look...2012, for example, 3 billion hours of video were watched each month, in 39 countries, and in over 54 languages... brief introduction to the research and, after watching the video to determine if you’d like to learn ...

...would ease people enough.  Build: If you’ve ever watched any behind the scenes footage in the last several...usually see a director sitting in their chair not watching their actors live on set, but peering at a 7-inch...more important lab work from being done. You can watch the first video in our Intro to the Lab Bench series...

...description to watch any sections you’re particularly interested in, but I’d recommend watching the whole ...own research needs. Other companies like NEB and ATCC can provide you with additional validated reagents...

...mutation to match a frequent mutation in early-onset GC and one with a mutation at R273H to match a mutation...EVs. Find ADAR-HiBiT Fusion Vector at Addgene! Watching protein degradation in real time Proteins involved...

... determine optimal DOL is through several small-batch experimental labeling. Size is also a factor. Bulkier... wavelengths and emission profiles, need to be matched with the experimental setup and objectives. Most... technical manuals. Specific applications and matching dye/ligand choices For effective live-cell imaging...

...checked. Assuming full complementarity, its sequence matches the guide sequence. PAM: The “protospacer-adjacent... and target in order to cut DNA, whereas some mismatches can be tolerated outside this region. Conclusion...

...CRISPR Genome Editing and The CRISPR Software Matchmaker Day 2: Homology directed repair: CRISPR 101: ...Mammalian Expression Systems and Delivery Methods and A Match Made in Heaven: CRISPR and AAV Day 6: Bacteria: ...

...(2017) AAV-ID: A Rapid and Robust Assay for Batch-to-Batch Consistency Evaluation of AAV Preparations....

...in your research.     To see Tetbow in action, watch the video below depicting dendrite wiring of mitral... and Takeshi Imai. "Optical clearing and index matching of tissue samples for High-resolution fluorescence...

...Plasmid Cloning Protocols Catch Up On Your Basic Molecular Biology Techniques Watch Our Protocol Videos ...

...single mismatch at any position of the DNA guide impaired NgAgo activity, with three mismatches abolishing...

... pathways. Individual cellular fates were then matched with candidate TFs responsible for that differentiation...collection, we’re releasing individual plasmids in batches. If there’s something you’d like that’s not yet...

...developing embryos are transparent, so it’s easy to watch development in real time.  In both cases, dL5** ...light activation. Video: https://www.youtube.com/watch?v=xOtH5bs4HiI Advantages of MG-2I-dL5** over other...

...polymerase) is the ability to remove, reset and ligate mismatched overhangs, yielding scarless DNA assembly, and...the reaction mixture. The exonuclease removes mismatched 3’ overhangs, so that the region of homology ...

...efficiently collected by the microscope. To compare the match between a fluorescent protein and a filter set, ... emission spectra are red. Neither is a perfect match to the filter set but the excitation filter excites...

... If that’s not enough, we consult with experts, watch webinars, or use other research methods. In the...repositories. Then we format, do a final review to catch those pesky typos, and hit publish. Making content...

...Did you catch our April AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If...packages of 10…)  If you missed the webinar, you can watch the presentation and Q&A session on our YouTube ...

...Which Cas9 Do I Choose for My CRISPR Experiment A Match Made in Heaven: CRISPR/Cas9 and AAV Resources on...Resources Find CRISPR Plasmids for Your Research Catch up on Your CRISPR Background with Our Guide Pages...

... neuronal activity, scientists can use GECIs to watch neurons fire under the microscope. To add another... usefulness depends on selecting a fluorophore matched to their excitation wavelength. OCaMP is well-suited...

...proprietary. These methods also rely on ssDNA ends to match up regions of homology, so they won’t work if the...L-arabinose. These cells can be made and frozen in large batches suitable for many experiments. When REPLACR-mutagenesis...

...enough senior people willing to step up and be matched with the many interested mentees. So I experimented...in preparation for the meeting or to be read or watched during the meeting.  Scientists do better with ...

...prevalent issue with polyclonal antibodies is their batch to batch variability. Once an animal has undergone a...

...typically evaluated with low-throughput methods such as patch-clamp electrophysiology, a technique that’s used...sequences were selected for characterization with patch clamp electrophysiology. All thirty were functional...

...quite variable from antibody to antibody or batch to batch. For example, take lysine conjugation, which...