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Showing: 51 - 100 of 444 results

  1. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...sequencing for quality control for over ten years. Now, thanks to a generous donation by the Hirsh family, we ...completes, we begin our assembly process. Again, thanks to our partners at seqWell we make use of a pipeline...labs often provide insert sequences or annotated Genbank files that are useful for more complicated plasmids...

  2. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ... don’t follow through on their promises, Wet-blanket negativist, who can bring down the morale of a ...hostile-aggressives, complainers, know-it all experts, wet-blanket negativists, and micromanagers), while other supervisors...book “How To Win Friends and Influence People.” Thank you to our guest blogger!  Dora Farkas received ...

  3. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...mini-transposon), which contains the DNA cargo of interest flanked by ~50-150 bp transposon end sequences (R and L...recognized during transposition, genetic cargos must be flanked by the L and R ends - thus, scarless insertions...CRISPR-transposons has yet to be fully realized. Many thanks to our guest blogger Leo Vo! Leo Vo is currently...

  4. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...particular structures (Table 1). Each construct was flanked with a CMV promoter (to drive high-level expression... contained in individual plasmids and are each flanked by the same multiple cloning site (MCS). Modules...original pattern of the MCS is regenerated at the flanks of each ligation product, which is the basis for...

  5. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...sequence (Figure 2A). Since you don’t need additional flanking sequences, shRNA plasmids are a bit smaller, with... than miRNA plasmids, which require additional flanking regions comprised of upstream and downstream genomic...between the red boxes. Highlighted blue regions show flanking genomic sequence. Created on BioRender.com. ...

  6. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...easily be attached with a hose to a nitrogen gas tank with a pressure regulator. Other methods for physical... new putative host cell-binding proteins! Many thanks to our guest blogger Jessica Sacher! Jessica Sacher...Twitter @JessicaSacher.   References 1. Nobrega, Franklin L., et al. "Revisiting phage therapy: new applications...

  7. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...the promoter to prevent its expression, and is flanked (“floxed”) by two sets of incompatible recognition...molecular mechanisms of the brain in vivo. Many thanks to our guest blogger Alessia Armezzani from genOway...233.39 (1971): 149. PubMed PMID: 4940442. Schnütgen, Frank, et al. "A directional strategy for monitoring Cre-mediated...

  8. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...sequence to flank a transgene (homologous pairs) or used different scrambled sequences to flank the transgenes...orientation and one of the unused (loxp) sites will be flanked by two lox sites now in the same orientation. Next...

  9. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...spreadsheet to [email protected] along with your GenBank files, plasmid sequences, and/or maps. Add plasmids...utilize your plasmids (these can be protocols, GenBank files, etc). We can accept sequence files in any...to include the total size of the insert and the GenBank ID associated with your insert. Pro Tip! If the...

  10. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...note: in this table, reprogramming efficiencies ranking as low, medium or high is based on the reprogramming...for deletion of the inserted transgenes that are flanked by loxP sites. While there is only a small loxP...donor plasmid contains the gene(s) of interest flanked by terminal repeat sequences, which the PB transposase...

  11. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    Published
    Jan. 31, 2018, 2:44 p.m.
    ...decorations, our holiday spirit remains. We would like to thank everyone who participated in our annual #Deckthelab... ever! & they totally succeed! WOW! #deckthelab Thank you to everyone who participated and congratulations...

  15. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    Published
    Oct. 8, 2019, 12:33 p.m.
    ...will be acknowledged on the data hub website as a thank you for your contribution to open science.  Please...share your thoughts through our Feedback Form.  Thank you for your participation in this resource! We ...

  16. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    Published
    March 16, 2017, 1:06 p.m.
    ...Different Audiences Listen to the SITN Podcast Many thanks to our guests, Vini Mani and Amy Gilson. Vini Mani... can follow Amy on twitter @aigilson.   Special thanks to our sound engineer/producer, Eddy Page! Edward...

  17. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    Published
    May 24, 2016, 2:30 p.m.
    ...Thoughts on his time in Boston and Outro We'd like to thank Dr. Kwapata for taking the time to talk to us and...recent collaboration with Seeding Labs here. Special thanks to our sound engineer/producer, Eddy Page! Edward...

  19. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    Published
    Nov. 16, 2020, 2:15 p.m.
    ...community the latest and greatest plasmid tools! Thank you depositors! In fact, this year, Addgene depositors...we crunched the numbers (shout out to Addgenie Priyanka Ramanathan for the data!) and found that plasmid...

  20. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    ...end! Their name is...(drum roll, please!)... Abi! Thank you so much to everyone who submitted names*, voted...their new friend…  Fig. 1: Meet...well, frankly, we're not sure yet!   This as-yet–unnamed ...

  22. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    Published
    Nov. 15, 2016, 3:30 p.m.
    ...Viral Service), and educational outreach. Many thanks to Alex Chavez for taking the time to sit down ...enable facile control of DNA and RNA.     Special thanks to our sound engineer/producer, Eddy Page! Edward...

  23. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...☎️ call up an old friend. Remind them you care!#thanks #health #mentalhealth https://t.co/Td2M6AOpuW pic.twitter.com... you with instructions to claim your prize. And thank you to everyone who entered. Keep an eye on Addgene...

  25. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    Published
    Sept. 1, 2016, 2:30 p.m.
    ...dreaded third year. Find Part 2 Here! We'd like to thank Niroshi and Ben for taking the time to sit down ... and reading Ben Vincent's tweets.      Special thanks to our sound engineer/producer, Eddy Page! Edward...

  27. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ...Precision and Plasticity in Mentoring" We'd like to thank Niroshi and Ben for taking the time to sit down ...biology and reading Ben Vincent's tweets.     Special thanks to our sound engineer/producer, Eddy Page! Edward...

  31. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...Shreya Vedantam, Michelle Cronin, and Angela Abitua Thanks to a team effort from the Addgenies, we’re able... a comment or emailing us at [email protected].  Thank you again for a fantastic year and happy reading...

  32. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...blog. Read the post for the full details and many thanks to Jessica for all of her hard work and attention...Trip in San Francisco and would like to give my thanks to all the Addgene supporters who spoke with me...

  33. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...received). Clearly we have all of our writers to thank for the excellent content they’ve churned out in... Impressions of NgAgo In particular, we need to thank Pooran Dewari who wrote this month’s most popular...

  35. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    Published
    Nov. 27, 2013, 2:54 p.m.
    ...the 400th plasmid that day.   As we head into Thanksgiving weekend in the United States, I look forward... a large table for a meal with my family. I am thankful for many things, and one is that I get to gather...

  37. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...proteins is easily accessible in the RCSB Protein Data Bank and PDBsum. After acquiring this information, you...22442034. Additional Resources RCSB Protein Data Bank - a database of detailed protein structural information...

  39. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ... Before we start, we'd like to extend a special thank you to Dr. Tom Ellis (Imperial College London), ...“Fun in the Lab!" and more.   Acknowledgments Thank you again to Connie Cepko, Tom Ellis, and George...

  40. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    .... In the destination plasmid, the ccdB gene is flanked by recombination sites. To introduce a gene of ...plasmid, recombination sites must first be added to flank the gene. Successful recombination and integration...

  41. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...section of this post. We feel GREAT when you send us thank you notes and recognition. You are why we do what...what we do. From all of us to all of you. Thanks for supporting nonprofit, open science and Addgene in...

  42. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...but overwhelmed; I had no idea where to start. Thankfully, my managers set aside time for me to complete... the office Just Dance competition.    Special thanks to my manager, Sr. Marketing Generalist, Steven...

  43. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...antibodies include:  Excellent tissue penetration, thanks to their small size of 12–15 kDa Ease of cloning... easier to generate and more widely adopted. So thank you to the camelids who inspired this versatile ...

  44. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ... individual portions of the manuscript. Stephen Blanke, Beronda Montgomery, or Louis Miller led these ...interesting introduction and concise discussion. Many thanks to our guest blogger Sean Stacey! Sean D. Stacey...

  46. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ... That is, all work from home as you are able... Thanks to everyone for being awesome during a tough time...research in laboratories all around the world." We thank the Chan Zuckerberg Initiative for supporting Addgene...

  48. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...technologies, and researchers behind them. As part of our thanks to you for being a part of our community, we’ve...guest posts by e-mailing us at [email protected]. Thank you again for a fantastic year and happy reading...

  49. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...Centralized reagent repositories, also known as biobanks, are crucial for the advancement of SARS-CoV-2...Japanese Collection of Research Bioresources Cell Bank, part of the National Institutes of Biomedical Innovation...

  50. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...gene-edited babies. Several hours later, the scientist, Jiankiu He, confirmed that two such edited babies were ...the necessary discussions and actions now.  Many thanks to our guest blogger, Kelly Hills! Kelly Hills...
Showing: 51 - 100 of 444 results