...as the spectrum shift occurs during Timer protein life due to a form of oxidation (dehydrogenation). MitoTimers...that you need. The Golden GATEway cloning kit simplifies the cloning process for complex DNA constructs...signaling dynamics are influenced by the relatively long life-cycle of a protein from expression to degradation...the same transgene as the Brainbow cassette, simplifying experiments when additional cross-breeding steps...between the vector and gene primer for SLIC cloning. Amplify the pCoofy vector and the sequence of gene of interest...cassette, which is not copied during the PCR amplification step, so that only vectors with the desired ...

...are having difficulty amplifying your target sequence, or if you want to amplify sequences from a large...efficient, but if you are having difficulties in amplifying a template, particularly from a large genome,...correct insert Sequence the regions that have been amplified by PCR, putting special attention in the areas...

...detected with tyramide signal amplification. Tyramide signal amplification occurs when HRP converts a fluorescently...Tyramide signal amplification of HRP Staining cultured cells and tissue sections Amplifying weak signals...

...and a Senior Research Associate at the Labor and Worklife Program at Harvard Law School. She recently launched...is a Senior Research Associate at the Labor and Worklife Program at Harvard Law School. He has continually...R. "A fair deal for PhD students and postdocs." Elife 2 (2013): e01139. Pubmed PMID: 24137543. Pubmed ...Marder, Eve. "Looking out for future scientists." eLife 3 (2014): e04901. Pubmed PMID: 25291257. Pubmed ...

... of my life, even though that’s what I thought,” she said. “I’m so excited to see where my life will take... as a research and technical assistant, when her life changed––she had a daughter. Needing a career switch...she loves to do, she would never work a day in her life. She said, “it now makes sense because I love working...

...for experimental use. Thus, the first step is to amplify your library to a concentration that is sufficient...next-generation sequencing to check the quality of your amplification. If you've obtained a ready-to-use lentiviral.... Figure 2: The CRISPR library must be amplified (A) to be used to generate lentivirus (B). Cas9... As such, sequencing CRISPR libraries after amplification and after a screen requires the use of next-...

...Expansion Microscopy (TREx). Elife 11:e73775. https://doi.org/10.7554/elife.73775  Dani A, Huang B, Bergan...Expansion microscopy of C. elegans. Elife 9:e46249. https://doi.org/10.7554/elife.46249  Additional resources ...

...inclusive. CBE Life Sciences Education. 13(4):602-606. Brian, D. 1996, Einstein: A Life. Chapter 17, Einstein... gains and positive STEM attitudes in CUREs. CBE Life Sciences Education. 21(4):ar74. Duboue, E., Kowalko...chemical biology, biochemistry, and neurobiology. CBE Life Sciences Education. 15(4):ar55. Ma, Y., Mukherjee...

...yes or no” kind of test, with a positive clone amplifying a product and a negative clone resulting in no...and without an insert to verify that the primers amplify the expected size PCR products.    Primer Design...same backbone plasmid. If this control doesn’t amplify a product, then you know there could be something...insert-specific primers. If your negative control amplifies a product of the expected size, you know the genome...

...beta platform for sharing, annotating, and storing life science protocols. Since we began sharing this protocol...Currently in beta testing, the software aims to be a life science protocol repository. The software can be...have a record of your work. This feature further simplifies lab work if you like to bring your phone in the...and track inventory and orders. Quartzy caters to life science labs and offers the service free of charge...

...species, even from completely different kingdoms of life. That’s convenient for researchers who want to do...episomal vectors is to include viral oris to drive amplification, which helps increase cellular uptake, but this... DNA intercalator, which affects all kingdoms of life. Of course, that includes you. Besides the risk ... you hop, skip, and jump between all kingdoms of life. A bit of careful design will help make your experiment...

...leading to genotypic and phenotypic artefacts. The life science literature is a litany of false interpretation...Down-Regulation of HIF-1α by NDRG2 Expression Attenuates Proliferation and Invasion in Renal Cancer Cells, Marker-independent...attributions (from top to bottom) Database Center for Life Science (DBCLS),  National Cancer Institute, Public... Skloot, Rebecca, and Bahni Turpin. The immortal life of Henrietta Lacks. New York: Crown Publishers, ...

...fusion proteins Biotin is an essential vitamin for life Although biotin is most commonly known for its role...essential coenzyme that is required by all organisms for life. Biotin is only synthesized by plants, most bacteria...sites found on avidin are also ideal for signal amplification during these experiments. During detection, ...bound to your protein of interest leading to amplification of the reporter signal and easier detection....

...by scFv antibodies fused to sfGFP. This system amplifies the intensity of fluorescence signal and enables...gene of interest. The gene to be expressed is amplified using a gene-specific forward primer containing... based on the Multisite Gateway® technology from Life Technologies. The MultiSite Gateway Kit is a ...ORFs from an ORFeome Gateway library or any PCR-amplified ORF cloned into pDONR221 can be expressed either...(MuLE) system from the laboratory of Ian Frew simplifies the process of creating complex polycistronic...iterative, chaining-based cloning method that simplifies the process of constructing specialized fluorescent...destabilizing sequences were added to shorten the half-life of both the luciferase mRNA (3’UTR AUUUA [5 consecutive...

...language. During the recession there were many qualified scientists with experience and a second language...carry out my dissertation research in a lab in California. I felt then, and still feel now, that it is ... do. I had taken advanced virology classes in California and enjoyed them, so I applied for a role at ... day planned out. This allowed for a better work-life balance compared to working in research. My previous...

...biomedical scientists and had no idea what the daily life of a scientist was like. The word “scientist” conjured...those scholarships that you don’t think you’re qualified for. Wayne Gretzky’s “You miss 100% of the shots... feel less confident in their abilities and qualifications. Studies on imposter syndrome have also found...and ended up with the award. We are often more qualified and capable than we know or believe we are. Funded...

...the scoop on the ups and down of graduate student life. Senior graduate students can sometimes be elusive...and talk to us and wish them the best of luck in life beyond graduate school. Ben J. Vincent is a Ph.D...fellow in clinical genetics at the University of California, Los Angeles. She enjoys chromosome biology and...

...reproducibility in biomedical research. eLife, 13, e100211. https://doi.org/10.7554/eLife.100211...Request, a service that dramatically expands and simplifies your viral vector options. Almost any AAV transfer...

...was certain I would not end up in a life sciences career but life has a way of making unexpected decisions...what it was like to work at Addgene. This is exemplified in my work interviewing fellow Addgenies. I spoke...

...that deserve a second look. 5 Great Apps for Lab Life This very useful post was contributed by guest blogger...growth medium, these apps can help make your lab life a bit easier. Since the post doesn’t provide an ... lab life...

...past few years, including an article in the BBC Wildlife Magazine. It is easy to see how these incredible...information on black screens, but not to know even the life cycle of the creature they deal with.” So photography...www.microworldsphotography.com/. Another artist who spent his life blurring the lines between science and art was Albert...

...bold. You don’t have to have all of the listed qualifications to apply for a position.  A job description...application). Beware the myths of scientist “overqualification” but it may not be productive to apply for...resumes The cover letter must address how you are qualified and why you are interested specifically in the...

...that I belonged there. Addgenies had a good work-life balance and were content. As a worker, there are...them. Addgene gave me the chance to become a real life scientist. Addgene gave me the chance to grow into... arms to me from day one. Addgene has changed my life for the better, and I wouldn’t want it any other...

..., allowing me to pinpoint areas of my career and life where I excel (and thus feel grateful) and identify...once said: “There are only two ways to live your life. One is as though nothing is a miracle. The other...quote encourages us to contemplate our approach to life and how our viewpoints influence our encounters....

...articles openly available on PubMed Central (also eLife, PLOS and Figshare). Some use cases include: Cataloging... collections from a large section of open access life sciences data. You can use ReFigure to coherently...we can only connect figures from PubMed Central, eLife, PLOS and Figshare but we are working on expanding...

...which limits its use for imaging a protein’s entire life-cycle. This makes it nearly impossible for scientists...permanently attached to a protein, making them hard to amplify and image for long periods of time.  Are antibody...demonstrated that the HA frankenbody can be used to amplify fluorescent signals in in vivo imaging: the 10xHA...

...with and/or requests materials from Addgene can qualify. We also reward improvement against one’s own record...Hospital  Brandeis University  Broad Institute  California Institute of Technology Cold Spring Harbor Laboratory...University of British Columbia  University of California, Irvine University of Massachusetts, Worcester...

...researcher on parasitoid wasps; and an essay on the life of Srinivasa Ramanujan, to be precise) and remarking...neurological health risk. Having played the sport my entire life, I was curious as to what the research had to say...chatting with individuals whose research ranged from wildlife conservation to abortion law to poetry. A research...

...multiplex immunolabeling of brain. eLife 8:e43322. https://doi.org/10.7554/elife.43322 Bandrowski, AE, Martone...created by James Trimmer, PhD, of the University of California Davis School of Medicine. This deposit includes...

...adeno-associated viral vector (AAV) samples and created a simplified process, termed viral genome sequencing (VGS)...viral genome sequencing workflow. Using this simplified approach, we sequenced over 100 AAV vector preparations...routinely producing AAV. We believe that this simplified sequencing and analysis approach can be easily...

...Individual Development Plan (IDP) and uses it to match qualified scientists to jobs in industry, academia, and ...find open positions and view their required qualifications and skill sets Taking the time to identify...Communication Networking As the number of qualified scientists entering the workforce increases, EAs...

...to target cells as a pooled library, which will amplify each TF to determine how its presence influences...processes. For help with library transformation and amplification, refer to the Zhang lab’s previously published...Addgene.org Plasmid Pooled Libraries Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome Library...

...multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:e43322. doi: 10.7554/eLife.43322. PMID: 30667360; PMCID...antibodies allows you to use them in parallel to help amplify your signal - giving you the benefit of a polyclonal...

...evidence (in psychology, cancer biology, pre-clinical life science work, economics) that published research... their study plans at a point in the research lifecycle when suggestions can be incorporated into the ...and as the Director of Advising in the Division of Life Sciences at Rutgers University. His dissertation...

...allowing the device to take on a community-driven life of its own. Nevertheless, there are still many open...Nibha Akireddy, Chew Chai, Manu Prakash, "Squid: Simplifying Quantitative Imaging Platform Development and... B., Marsikova, B. et al. Open microscopy in the life sciences: quo vadis?. Nat Methods 19, 1020–1025 ...

...with the desired mutation, then use those to PCR amplify the template plasmid. The parental, nonmutated ...primers, each containing the desired mutations, to amplify both strands of a plasmid template. In addition...pitfall of REPLACR-mutagenesis is that you must PCR amplify the whole plasmid, which could introduce mutations...

...strands take part in rolling circle DNA amplification. The amplified DNA segment is labeled with fluorescent...directed toward AHA. Now the rolling circle amplification could only take place if the two antibodies ...

...plasmid (Option 1) or added using PCR-based amplification (Option 2). Advantages of Golden Gate cloning...is to "domesticate" your fragment: PCR-based amplification can be used to create silent point mutations...high-efficiency step. References: Sequential amplification of cloned DNA as tandem multimers using class-IIS...

...antibody, a primary-secondary pair is used. This amplifies the signal as multiple secondary antibodies can...ELISA, the only difference being the additional amplification step, which helps to visualize low-abundance...DNA damage and instability detection. Cell. Mol. Life Sci. 76, 4689–4704 (2019). https://doi.org/10.1007...

... I possess.   Bullet points on resumes are like cliff notes, so I use these as a starting place for drafting...technical and transferable skills that make you qualified for the position. Highlight the outcomes or deliverables... have made it to the interview if you weren’t qualified for the position. So prepare for the interview...

... dozen versions of the toehold switches and amplification primers. This helped make sure that we had a...this includes primer directed isothermal RNA amplification, new toehold switch designs, CRISPR/Cas9-mediated...global environmental suitability for Zika virus." Elife 5 (2016): e15272. PubMed PMID: 27090089. Resources...

...work on the origin of cellular life. His lab has long hypothesized that life started with RNA and a simple...cautioned the distinction of “higher” and “lower” life forms as we shared millions of years of common ancestry...

...reduced their lifespan. Because these dramatic conditions were experienced in a period of life when the grandparents...statistically significantly associated with reduced life-span, but no changes in the DNA sequence could be...

...reference sequence and the gene of interest to be amplified in the same reaction. Taqman’s increased sensitivity...of interest Some gene sequences are harder to amplify and quantify than others. If available, ddPCR may...chain reaction methods for the determination of amplification of Plasmodium falciparum multidrug resistance...

... industry early would only help. Like any major life decision, the transition felt scary. While I knew...scientific discussion is often part of your daily life. Also, while you’re unlikely to be laid off as an...never-ending cycle of grant-writing that is academic life. Of course, because you will be part of a team in...

...exposure under these conditions once the measures were lifted (Servellita et al., 2023).  This anecdote goes ...seven species, designated as letters A–G) exist. The life cycle of AdVs is dependent on modulation of the ... et al., 2003).        Figure 1: The adenovirus life cycle. Image reused from Kremer and Nemerow 2015...

...controlled timing of CRISPR/Cas9 delivery. eLIFE. (2014) 10.7554/eLife.04766 Moreno-Mateos, M. A., Fernandez... timing of this approach carefully (Lin et al., eLife)! Use a Cas that facilitates HR What if promoting...

...enable the ability to study life’s innovations from anywhere in the tree of life.” Accepted proposals were...hydra and hydra have asexual and sexual reproductive life cycles. One can inject plasmid DNA in the eggs to...

...multiplex immunolabeling of brain. eLife, 8, e43322. doi: 10.7554/eLife.43322. PMID: 30667360....also make CAR-T cell therapy more accessible by simplifying the production process and could even enable ...

...within the scientific community, as they allow for simplified transformation processes, and can be used by ...Gram-negative  Alcaligenes eutrophus, Bartonella bacilliformis, Bordetella spp., Brucella spp., Caulobacter...expression in bacteria. Methods in Molecular Biology (Clifton, N.J.), 765, 327–343. https://doi.org/10.1007/978...