Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 317 results for: pho

Showing: 51 - 100 of 317 results

  1. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...-fold more photostable and as bright to mVenus, the parent YFP, and is the most photostable YFP to date...improved activity as compared to other recombinase/photodimer pairs. Created with BioRender.com In their... publication, Mary Dunlop’s lab generated a photosensitive Cre, Opto-Cre-Vvd, by splitting and joining...joining the protein regions with Vivid (Vvd) photodimers. Upon the application of blue light, Vvd dimerizes....ncbi.nlm.nih.gov/32675376/ mGold, the most photostable yellow fluorescent protein to date By Justin ...contribute to experiments that require high photostability, such as time-lapse imaging or with high light...

  2. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...I’ve answered hundreds of phone calls and thousands of customer service emails in my six years as a senior...now spend far less time answering help emails and phone calls, I feel compelled to share my years of accumulated...customer, can get the most out of your email or phone call. Though my experience is based solely on my...texting, actually writing an e-mail or making a phone call is a big commitment when you’re busy. Your ...might think. The Addgene scientists answering the phone are smart, but they cannot (and should not) answer... our thread or remove a good 5 minutes from our phone call. If your concern is based on data you have... Abcam and NEB have Ph.D.s answering emails and phones). We can’t be experts on every technique or experiment...

  3. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...cells lines have similar morphologies, being familiar with these morphologies and doing a quick check ...that any medium or washing buffer, such as PBS (Phosphate Buffered Saline), that comes in contact with the...purpose.Learn about the effects of CO2 on sample pH Cell morphology: Shape and size matter You should have a light...incubator). It also helps to get familiar with the morphology of the cell line you’re working with so that ...passaged. This could change their growth rates and morphology, rendering their continued use inadvisable for...passage number. Cells change their growth rate and morphology at high passage numbers (>30-40 times), depending...

  4. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...an opsin (a GPCR) linked to a chromophore and functions as a photoreceptor in the eye. There are two flavors...voltage-gated potassium channels found in Drosophila to phosphatases from sea squirts. This design structurally separates...cell level as they are in vitro. Factors like photobleaching, lack of red-shifted indicators, and brightness...significantly brighter and available with red-shifted fluorophores (find FRET capable GEVIs here!). The only issue...issue with these GEVIs? They perform poorly in two-photon conditions, which are necessary conditions for ...indicator. To give an example, a genetically encoded phosphatase (voltage sensing) hydrolyzes a precursor dye ...

  5. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...all of the fluorophores as it passes through the specimen, which often leads to photobleaching.  Furthermore...focus light. Therefore, there is much less photo-bleaching or photo-damage than in conventional laser scanning...order to improve axial resolution and reduce phototoxicity for imaging live specimens, they combined light...light scattering and absorption.  Finally, the photomultipliers used to detect light in confocal microscopes...obstacles (e.g. air bubbles or high concentration of fluorophores) cast shadows along the illumination path. The...

  6. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...identification of plasmid multimers. Capillary Gel Electrophoresis (CGE) analysis Another technique that can detect... detect multimers is capillary gel electrophoresis (CGE), which has been used in plasmid vaccine and gene...Plasmid Topology Monitoring by Capillary Gel Electrophoresis. Curr Mol Med. 2020;20(10):798-805. doi: 10.2174... He Y, Lacher NA, Ruesch MN. Capillary gel electrophoresis with laser-induced fluorescence of plasmid ...plasmid DNA in untreated capillary. Electrophoresis. 2010 Jul;31(14):2436-41. doi: 10.1002/elps.201000061. PMID...topoisomers, knots, and catenanes by agarose gel electrophoresis. Methods Mol Biol. 2009;582:11-25. doi: 10.1007...topology analysis of plasmid DNA by capillary gel electrophoresis. BioProcess Int. 2006 Sep;4(8):38-40. Summers...

  7. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ... Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is the first step of a western. ...s post-translational modifications such as phosphorylation, acetylation, methylation and ubiquitination...ubiquitination. For example, to test if a protein is phosphorylated following a specific experimental condition you....2015.04.012 Burnette WN (1981) “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide....76.7.3116 Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels...

  8. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ... construct for every application. Plus, the photophysical properties of chemical dyes provide many advantages...strategies. The green structure represents a fluorophore or other useful ligand, gray structure is the...non-natural amino acids to directly incorporate a fluorophore in your protein or to incorporate an alkene to...Organic dyes are typically both brighter and more photostable than fluorescent proteins (often up to 10-fold...years; improving the labeling kinetics or the fluorophore’s microenvironment to enhance brightness, stability...Better Dyeing Through Chemistry & Small Molecule Fluorophores. Addgene Blog. https://blog.addgene.org/better-dyeing-through-chemistry-small-molecule-fluorophores...better-dyeing-through-chemistry-small-molecule-fluorophores Los, G. V., Encell, L. P., McDougall, M. G.,...

  9. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...sensor’s photophysical properties such as kinetics, ∆F/F0, SNR, dynamic range, photostability, quantum...signals upon sensing glutamate using both 1-photon and 2-photon imaging.   Figure 1: 1P and 2P excitation...membrane trafficking and localization. Improved photophysical properties The improved variants of iGluSnFR3...pMinDisplay vector. GPI contains a C-terminal glycosylphostidylinositol anchor. SGZ contains a PDGFR transmembrane...

  10. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Published
    July 27, 2017, 1:11 p.m.
    ...Xin Zhou greatly expanded that list by using photoswitchable fluorescent proteins to create enzymes that...turned the enzymes back on. Xin termed these photoswitchable enzymes Fluorescent Light-inducible Proteins...various designs, Xin has been able to develop photoswitchable Intersectin, Cdc42, Raf1, Mek1, Mek2, Cdk5,... You can learn more about Xin’s work with photoswitchable components of the ERK signaling pathway in ... the production of HMOs. Current HPLC-MS and fluorophore-based technologies are too time consuming in ...Resources on the Addgene Blog Learn More about Photosactivatable Fluorescent Proteins Use Light to Control ...

  11. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...envelope, Phoenix-ECO, (for infection of mouse and rat cells), or an amphotropic envelope, Phoenix-AMPHO (for...of the viral packaging cell line. For example, Phoenix, a second generation γ-retrovirus packaging cell... produced in just a few days. Another variant, Phoenix-gp, contains only Gag-Pol and enables additional...

  12. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ... efficiency by which absorbed photons are converted into emitted photons. These are indicated by the extinction...this series of posts are (i) brightness, (ii) photostability and (iii) aggregation tendency. Brightness...high brightness is an important feature of a fluorophore. The key question is how the brightness is best...Theoretical brightness Two key properties of fluorophores that determine brightness are the extent to ...Without further information about properties like photostability or aggregation tendency, our choice would be...

  13. Participate in the Pets of Addgene Scavenger Hunt

    Type
    Blog Post
    Published
    April 1, 2021, 1:15 p.m.
    ...clue 1 below!) You will find five photos this way. At the very last photo, you will find a link to a form...workspace over the last year. Now, we’ve collected photos of our pets with Addgene gear, and have unleashed...Addgene-branded clothing on the blog. Below each photo, the pet gives you a clue to help you find the next... next picture. If you find the next correct photo, you will see a reference to this scavenger hunt and...

  14. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...of which you can find at Addgene. Biliverdin chromophore Near-infrared fluorescent proteins (NIR FPs) ...) were derived from natural photoreceptors incorporating biliverdin IXα (BV) autocatalytically. Fortunately...from your source and to your camera.  Two-photon image of miRFP670nano3-expressing neurons in the... derived from bacterial phytochrome (BphPs) photoreceptors have higher apparent cellular brightness than...were derived from cyanobacteriochrome (CBCR) photoreceptors (Oliinyk et al., 2019). They were engineered...Molecular brightness vs. iRFP713, % Oligomeric state Photo-stabilityc, t1/2, s pKa Brightness in HeLa cells...

  15. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...et al., 2013), incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red) to solve...expect to see systems that use newer, more photostable fluorophores and that can be combined with new imaging...their technology Brainbow. Brainbow 1.0 used the fluorophores RFP (red), YFP (yellow), and membrane-tethered...clearing methods and can be labeled by synthetic fluorophores, which are more stable than fluorescent proteins...

  16. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...inactivate the fluorophores in the sample, either by chemical treatment or photobleaching or by stripping...and that each secondary has a different color fluorophore that you can image separately on your microscope...labeling sequentially with one, inactivating the fluorophores, then labeling with the other, you can still...using primary antibodies that are conjugated to fluorophores. However, the primary-secondary workflow (a....fluorescently-conjugated streptavidin to get more fluorophores per antibody, or conjugate with an enzymatic...

  17. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ...last year, Verkhusha added two far-red light photoactivatable (PA) near-infrared fluorescent proteins (FPs...fluorescent proteins, iRFP670 and iRFP720, as photoacoustic contrast agents for two-color imaging in animals...genetically-encoded probes by making them brighter, more photostable, smaller, and less toxic to cells. They’d like...751-4. doi:10.1038/nmeth.2521) Far-red light photoactivatable near-infrared fluorescent proteins engineered...: 10.1038/ncomms3153. PubMed) Multicontrast photoacoustic in vivo imaging using near-infrared fluorescent...

  18. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...for up to 60% of the cell culture contamination (Uphoff, 2002). Mycoplasmas are considered to be one of...from lowered pH due to microbial contamination. Photo by Kaustubh Kishor Jadhav. Mycoplasmas can remain...for at least two or three days before sampling (Uphoff and Drexler, 2011). Culturing on agar plates/broth...works in the tissue culture hood while wearing PPE. Photo by Kate Harten DeMaio. Cell lines and reagents... of mycoplasma species. J Microbiol .44: 42–49  Uphoff Cc, Drexler Hg (2002) Comparative Antibiotic Eradication...10.1290/1071-2690(2002)038<0086:caeomi>2.0.co;2 Uphoff CC, Drexler HG (2011) Detecting Mycoplasma Contamination...

  19. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...SL-insensitive fluorophore (LSSmOrange) as an internal control and a SL-sensitive fluorophore (circularly...three-dimensional cultures that mimic aspects of organ morphology and physiology in vitro and have emerged as a...investigating genetic mechanisms related to morphogenesis, cell fate specification, and human disease....relies on the fluorescence ratio between the 2 fluorophores, rather than on the fluorescence intensity, ...

  20. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...signal-to-noise ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule...enough signal. This workaround risks cellular phototoxicity and eliminates the possibility of long-term ...a higher signal-to-noise ratio with a lower photobleaching rate. Given the power of SunTag, they attempted...Protein Oligomerization Learn about Small Molecule Fluorphores Resources on Addgene.org Find Fluorescent Protein...

  21. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...contributed by Leah Schwiesow Calcineurin is a protein phosphatase that responds to rising intracellular calcium... of Ca2+ are high in the cell, calcineurin dephosphorylates the protein CZI, which in turn binds to calcineurin-dependent... intercalating fluorescent dyes which can cause photo-induced DNA breakage and structural deformation,...reversible and do not cause structural deformation or photo-induced damage. These fusions can also function ...

  22. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...fluorescent protein joins mTFP1 and mWasabi, as fluorophores from Allele Biotechnology that now can be deposited...brightest teal fluorescent proteins that were photostable and had a high 480/530 nm emission ratio. They...mWasabi is 1.6-fold brighter than EGFP and is photostable. It also has narrow excitation and emission peaks...useful in multicolor labeling particularly with fluorophores that excite in the violet range. For example...Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein...

  23. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    Published
    Sept. 18, 2018, 1:33 p.m.
    ...migration of a cancer cell line equipped with photodissociable EB1 could be stopped in areas with blue light...manipulating and studying cellular dynamics. The photoinducible control of protein function is more specific...signal. Prior to the development of these probes, fluorophore binding aptamers and MS2-binding fluorescent ...contain a smaller RNA tag, are less prone to photobleaching, and achieve better signal in vivo as compared...aptamers or MS2 tags using the same number of fluorophores. You can find plasmids containing the cobalamin...

  24. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...sfCherry2 sfCherry2(1-10) and sfCherry2(11), also photo-activatable variant PAsfCherry2(1-10) Improved split...iRFP713. Requires presence of heme oxygenase for chromophore formation (abundant in eukaryotes) A Near-Infrared...domain. Requires presence of heme oxygenase for chromophore formation (abundant in eukaryotes) Bright monomeric...typically irreversible and requires some time for chromophore maturation, splitFAST displays rapid and reversible...flexibility in color labeling with exogenous fluorophores, which can even be bright enough for single-...

  25. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ... molecules, which are much brighter and more photostable than fluorescent proteins, making them ideal ...versions. ASAP5 can be imaged using one- or two-photon microscopy to detect action potentials and subthreshold...fluctuations in awake behaving mice through two-photon microscopy at depths up to 600 µm. Figure...recognizes the disease-associated version of Tau (phospho-Tau). We hope these will become valuable tools ...

  26. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...example, if your flow cytometer reads out the fluorophores FITC and PE on the same laser, you will need...deciding to use them, using a tool like BioLegend’s Fluorophore Guide Chart.  Pro tip! Some antibodies go by ...change the compensation values for whichever fluorophore is present in the sample (APC in the case of ...other channels you’re using first. A different fluorophore channel may be affecting your populations and... this population is double-positive for both fluorophores and that there is no issue with compensation...

  27. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...NgAgo DNA guide is inexpensive and can be 5’-phosphorylated in house using a simple PNK reaction or can... the instability of the supposedly alien 5’-phosphorylated ssDNA guides, which might be rapidly degraded...things (101 lessons on Spanish, C++, guitar, photography) and often dreams of a sunny day in Edinburgh...

  28. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...molecules and coaxes it into sticking to the hydrophobic glass filter. Each glass filter can capture up... only requires incubating the columns with 1M phosphoric acid for 3 minutes followed by a short centrifugation...purification columns could also be cleaned with 1M phosphoric acid and re-used, but trace contamination could...

  29. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ... Figure 1: Plasmid mix to label neuronal morphology (eYFP) and the synaptic protein PSD95 (PSD95-...control   Figure 2: Expression of a morphological marker (eYFP) and synaptic marker (PSD95-mcherry...experiment it is possible to compare neuronal morphology and synaptic connectivity before and after the...Micron Plasmid Attaches Covalently to DNA via a Phosphotyrosyl Linkage.” Molecular and Cellular Biology 5(...

  31. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...well-studied kinase known to prevent tau hyperphosphorylation. You could run brain samples from both groups... molecular weight shifts indicative of tau phosphorylation. By including the positive control, you will...controls are single stained samples for each fluorophore and are critical to analyze multiplex flow cytometry...

  32. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ... the phosphatase you choose. CIP (calf alkaline phosphatase) or SAP (shrimp alkaline phosphatase) are ...overhangs after digestion, you will need to use a phosphatase to prevent re-circularization of the backbone... treat your digested backbone plasmid with a phosphatase prior to the ligation step or prior to the gel...

  33. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...CRISPR variant going forward.   Special Delivery Fluorophore Targeting for FRET Studies While I love all of...This post describes how to target and use FRET flurophores for a variety of applications. While editing ... to study biological phenomena and about how fluorophores other than fluorescent proteins can be incredibly...Comparison to Cas9 and NgAgo Special Delivery: Fluorophore Targeting for FRET Studies Gendered Innovations...

  34. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...et al. "A rhodopsin is the functional photoreceptor for phototaxis in the unicellular eukaryote Chlamydomonas...Deisseroth, combined optical and genetic methods to photo-stimulate mammalian neurons and alter synaptic events...rhodopsins, light-sensitive proteins that absorb photons for energy conversion or the induction of intra...resulting viral-infected neurons are subsequently photo-stimulated through fiberoptic cannulas directly ...

  35. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...started!   Thanks for all your hard work!   Fluorophores When is a Monomer not a Monomer? The Top Three...Better Dyeing Through Chemistry & Small Molecule Fluorophores In this post, Luke Lavis from Janelia Research...fluorescent proteins are incredibly useful, chemical fluorophores can still be used in a wide variety of experiments...experiments. Read more to find chemical fluorophores for your next experiment. Nucleic acid based machines...

  36. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...protein could cleave DNA in vitro, directed by phosphorylated DNA guides. Unfortunately, Swarts et al. were...in GC-rich regions. DNA guides NgAgo uses 5’ phosphorylated DNA guides (so called “gDNAs”) rather than ...

  37. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...assume that anyone reading this article has a smartphone nearby. We tailor these devices to our own interests...have relied more and more heavily on adapting my smartphone to help me with my research in terms of preparedness...instead conveniently provide them on your smart phone. 1. Lab Archives by LabArchives The single, most...with colleagues and link your notebook from your smartphone to your computer. Lab Archives will help both...

  38. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...questions. Don't try to get all panelists on the phone at once – you won't get as much out of it and it...better at remembering these things on a relaxed phone call than when first confronted on a stage in front...front so the interactions are better. Have a good microphone system ready for each panelist if the room needs...what happens to the attention of the audience – phones come out, postures shift, they are checking out...

  39. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ... wavelengths of low photosynthetic activity. This still allows photosynthetically active wavelengths to... coral’s symbiotic dinoflagellates that are photosynthetic (Salih et al., 2000). Chromoproteins also enhance... (2000) Fluorescent pigments in corals are photoprotective. Nature 408:850–853 . https://doi.org/10.1038...

  40. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...and ecology can make for beautifully compelling photos and videos, while molecular, micro, and cell biology...Easy to host guests remotely. Easy access from smartphones with low data requirements.  No visuals. Variety...academic to informal writing can difficult.  Photography Medium (high for microscopy) Visually compelling...context via still images. Some things cannot be photographed or need text to properly contextualize. Graphics...

  41. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    Published
    Aug. 13, 2019, 12:58 p.m.
    ... oligonucleotides called morpholinos. When injected into zygotes, morpholinos complementary to a target...activity and does not work easily in zebrafish.) Morpholinos are a convenient and fast way to deactivate genes...whether a gene is deactivated via mutation or morpholino. This observation unlocked novel research into...

  42. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...fiber photometry.  Figure 1: Characterization of iGluSnFR4 in mice. A) Scheme for two-photon (2P)...iGluSnFR4s produces larger signal-to-noise ratios in two-photon imaging, making it ideal for video recordings of...dTomato, displaying characteristic PV basket cell morphology. Adapted from Furlanis, Dai, Leyva Garcia et ...

  43. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...fabulous photos again this year! Why #DecktheLab? Help us spread some cheer by snapping a photo of you... Festivus— you name it, we want to see it! All photos tagged with #DeckTheLab throughout the month of...

  44. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...effector cells of the immune system - cytotoxic T lymphocytes (CTLs), natural killer (NK) cells, and helper...consistently shown promise in reducing leukemia and lymphoma and are in their clinical trial phase. In all ... this results in frequent physiological and morphological changes. Tumors differ in their genotypic and...expressing CD19 chimeric antigen receptors for acute lymphoblastic leukaemia in children and young adults: a phase...

  45. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ... primary antibodies that are conjugated to a fluorophore, to avoid the use of secondary antibodies in .... Because the signal comes from the antibody-fluorophore conjugate, and is not expressed by the cell itself...localization. These could be visualized using fluorophore-conjugated antibodies (i.e., immunofluorescence...unconjugated primary antibody, then visualized using a fluorophore-conjugated secondary antibody or Fab. Image from...

  46. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...Halloween festivities for years – searching our photo archives I found pictures back through 2009, though...contests were indeed occurring earlier but that the photo evidence was destroyed to protect the innocent revelers...Facebook and checkout our FB page on Friday for more photo evidence.        ...

  47. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...often used to monitor enzymatic activity or phosphorylation over time. You can also use biosensors to follow... Rosella Autophagy Sensor Read about Aptamer Fluorophores Use CRISPRainbow for Genome Visualization Resources...

  48. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...unmodified LC3, GFP-LC3 is conjugated to phosphatidylethanolamine (PE) and recruited to autophagasomal membranes...Takeshi, and Daniel J. Klionsky. "The quantitative Pho8Δ60 assay of nonspecific autophagy." Methods in enzymology...protein oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy Resources...

  49. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...inside cells that are on the move, such as T lymphocytes. Griesbeck and his colleagues began by isolating...and high resolution functional tracking of T lymphocytes, too, making them a versatile tool for application... functional in vivo imaging of neurons and T lymphocytes. Nat Methods. 2014 Jan 5. doi: 10.1038/nmeth.2773...
Showing: 51 - 100 of 317 results