We narrowed to 112 results for: 002
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TypeBlog PostPublishedSept. 21, 2023, 1:15 p.m....Cytochem 62:197–204. https://doi.org/10.1369/0022155413511620 Lu QL, Partridge TA (1998). A New Blocking...Cytochem 46:977–983. https://doi.org/10.1177/002215549804600813 Tuson JR, Pascoe EW, Jacob DA (1990) A novel...
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The Importance of High Titer for AAV Transductions
TypeBlog PostPublishedMarch 1, 2018, 2:11 p.m....virus vector production." Kidney international 61.1 (2002): S9-S15. PubMed PMID: 11841606. 2. Schultz BR, ...1189-1199. doi:10.1038/mt.2008.103. PubMed PMID: 18500252. PubMed Central PMCID: PMC2574934. 3. Zingg, Brian... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog PostPublishedJune 23, 2022, 1:15 p.m....Journal of cellular physiology. https://doi.org/10.1002/jcp.30064 2. Barman et al. 2020. “A glance at ...technology.” Current genetics. https://doi.org/10.1007/s00294-019-01040-3 3. Khatibi et al. 2021. “CRISPR Genome... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog PostPublishedFeb. 11, 2021, 2:15 p.m....different human genes encoding GPCRs (Takeda et al., 2002). G proteins: The receptor is coupled with heterotrimeric...Takeda S, Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of G protein-coupled receptor genes... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m.... 104:5163–5168 . https://doi.org/10.1073/pnas.0700293104 Field J, Nikawa J, Broek D, MacDonald B, Rodgers...Protocols in Protein Science 73: . https://doi.org/10.1002/0471140864.ps0909s73 Additional references on the... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog PostPublishedAug. 9, 2022, 1:15 p.m....Research. PLoS Biol 13(6): e1002165. https://doi.org/10.1371/journal.pbio.1002165 More resources on the Addgene... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m....Shaw, G., Morse, S., Ararat, M., & Graham, F. L. (2002). Preferential transformation of human neuronal ....2021.796991. Urabe, M., Ding, C., & Kotin, R.M. (2002). Insect cells as a factory to produce adeno-associated... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....zipper and Rel transcription factors (Hu et al., 2002). This would be more difficult with FRET, as the...10.7554/elife.38853 Hu C-D, Chinenov Y, Kerppola TK (2002) Visualization of Interactions among bZIP and Rel... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m....Virol., 73 (Pt 3), 653–660. https://doi.org/10.1099/0022-1317-73-3-653. PMID 1372038. Randall, R. E., Young..., 68 (Pt 11), 2769–2780. https://doi.org/10.1099/0022-1317-68-11-2769. PMID 2445904. New tool for ... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog PostPublishedAug. 23, 2018, 12:05 p.m....monomeric EGFP variant, mEGFP (Zacharias et al., 2002). GFP needs oxygen Chromophore formation requires...membrane microdomains of live cells." Science 296.5569 (2002): 913-916. PubMed PMID: 11988576. 2. Tsien, Roger... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog PostPublishedAug. 15, 2019, 12:30 p.m....Introduction of the strong transcriptional terminator BBa_B1002 (from IGEM) upstream of the MCS to reduce transcriptional...Microbiol. 81.15 (2015): 5103-5114. PubMed PMID: 26002895. PubMed Central PMCID: PMC4495200. Reisch, Chris... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog PostPublishedMay 13, 2025, 1:15 p.m....transcriptionally active regions of the genome (Schröder et al., 2002), thus increasing the risk for insertional mutagenesis...Chen, H., Berry, C., Ecker, J. R., & Bushman, F. (2002). HIV-1 integration in the human genome favors active... -
Plasmids 101: Biotinylation
TypeBlog PostPublishedNov. 15, 2018, 1:50 p.m....vitamin.” American Journal of Clinical Nutrition 75.2 (2002): 179–180. PubMed PMID: 11815306. 4. Nicole Kresge... PubMed PMID: 26224555. PubMed Central PMCID: PMC4884002. 10. Boer, E. de, Rodriguez, P., Bonte, E., Krijgsveld... -
Viral Vectors 101: Systemic Capsids
TypeBlog PostPublishedOct. 12, 2023, 1:15 p.m....barrier. PLoS Biology, 21(7), e3002112. https://doi.org/10.1371/journal.pbio.3002112 Jang, M. J., Coughlin, ...1), 31-50.e8. https://doi.org/10.1016/j.medj.2022.11.002 Tabebordbar, M., Lagerborg, K. A., Stanton, A... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog PostPublishedJuly 12, 2016, 2:30 p.m....Protocols in Human Genetics 88: . https://doi.org/10.1002/0471142905.hg1508s88 Leidy-Davis T, Cheng K, Goodwin...Protocols in Mouse Biology 6:39–66 . https://doi.org/10.1002/9780470942390.mo150178 Qin W, Dion SL, Kutny PM... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m.... PubMed PMID: 24700321. PubMed Central PMCID: PMC4100247. Taylor, Norman E., et al. "Optogenetic activation... pharmacology 75.1 (2016): 11-19. PubMed PMID: 27960028. Van Duyne, Gregory D. "A structural view of cre-loxp... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog PostPublishedApril 16, 2024, 1:15 p.m....Biology, 3(6), 373–382. https://doi.org/10.1021/cb800025k Miller, L. W., Cai, Y., Sheetz, M. P., & Cornish...2560–2575. https://doi.org/10.1021/acs.biochem.1c00258 More resources from Addgene Better Dyeing Through... -
A History of Genome Engineering in Popular Culture
TypeBlog PostPublishedFeb. 25, 2020, 2:15 p.m....Hygiene 27:113–159 . https://doi.org/10.1017/s0022172400031879 Hershey AD, Chase M (1952) INDEPENDENT FUNCTIONS...https://doi.org/10.1038/nprot.2013.143 Seligman LM (2002) Mutations altering the cleavage specificity of ... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog PostPublishedMarch 14, 2017, 2:30 p.m.... reduction as well as progression. Dunn et al in 2002, introduced the “Three Es of Immunoediting” nomenclature...immunosurveillance to tumor escape." Nature immunology 3.11 (2002): 991-998. PubMed PMID: 12407406. 4. Weiner, George... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog PostPublishedSept. 10, 2015, 2:30 p.m.... [et al]. 2012;Chapter 1:Unit 1 20 1-18. doi: 10.1002/0471142301.ns0120s59. PMID: 22470147. 6. Gore ...Crawley [et al]. 2013;4(435):4 35 1-4 20. doi: 10.1002/0471142301.ns0435s65. PMID: 25429312; PMCID: PMC4242517...