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We narrowed to 135 results for: 002

Showing: 1 - 20 of 135 results

  1. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...Microbiol Biotechnol. (2003) 60:523-533 DOI: 10.1007/s00253-002-1158-6 Baker, M., Reproducibility crisis: Blame...

  2. Genetic Code Expansion

    Type
    Collection
    ... Huber 200225 pMega-MaPylRS PylRS M. alvus Nε-Boc-lysine Bacterial TAG Alanna Schepartz 200226 pMega-MaFRSA...amino-propanoic acid (Anap) Mammalian TAG Simon Elsaesser 140020 pAS_4xMma PylT_FLAG-AbKRS-chIPYE chimeric PylRS...photo-crosslinking-lysine Mammalian TAG Simon Elsaesser 140021 pAS_4xMma PylT_FLAG-AcKRS AcKRS M. mazei acetyl-lysine...acetyl-lysine Mammalian TAG Simon Elsaesser 140022 pAS_4xMma PylT_FLAG-PcKRS PcKRS M. barkeri photocaged-lysine...-lysine Mammalian TAG Simon Elsaesser 140023 pAS_4xMma PylT_FLAG-Mma PylRS AF PylRS AF M. mazei Mammalian...

  3. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Curr Protoc Neurosci. 2020 Dec;94(1):e106. doi: 10.1002/cpns.106. PMID: 33151027; PMCID: PMC7665837. L'...Immunol. 2013 Jul 29;4:217. doi: 10.3389/fimmu.2013.00217. PMID: 23908655; PMCID: PMC3725456. Schwarz H...Biotechnol Bioeng. 2021 Sep;118(9):3499-3510. doi: 10.1002/bit.27776. Epub 2021 May 4. PMID: 33811659. Pace...protein. Protein Sci. 1995 Nov;4(11):2411-23. doi: 10.1002/pro.5560041120. PMID: 8563639; PMCID: PMC2143013...ISSN 1359-0294, https://doi.org/10.1016/j.cocis.2014.10.002. Randolph TW, Schiltz E, Sederstrom D, Steinmann...formation. J Pharm Sci. 2015 Feb;104(2):602-11. doi: 10.1002/jps.24259. Epub 2014 Nov 21. PMID: 25418950; PMCID...

  4. Deep Dive: qPCR

    Type
    Blog Post
    ...reference genes whenever possible (Vandesompele, 2002), and it's possible to multiplex your reference ...issues as a reference gene (Higgins, 2000; Ref, Zhu, 2002), though there are cases in which it is an appropriate...in the literature, a geNorm study (Vandesompele, 2002) will help you select the appropriate reference ...re-optimize protocols for that machine. (Teo, et al, 2002; see paper for more information on optimizing for...Biotechniques. 2000 Aug;29(2):332-7. doi: 10.2144/00292rv02. PMID: 10948434.Taylor SC; Laperriere G;  Germain...0167-7799, https://doi.org/10.1016/j.tibtech.2018.12.002.Teo IA; Choi JW;  Morlese J; Taylor G; Shaun ...target DNA. Journal of Immunological Methods 270 (2002) 119 – 133Tichopad A; Dilger M; Schwarz G; Pfaffl...

  5. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ... thaw cycle (Krajden et al., 1999;  Ugai et al., 2002). If the virus will be used in a couple of days,... two negatively-charged membranes (Davis et al., 2002). Polybrene is one such cationic reagent that is...transduction efficiency by 10-fold (Davis et al., 2002). However, polybrene is highly sensitive to freeze-thaw...delivery." Japanese journal of cancer research 93.5 (2002): 598-603. PubMed PMID: 12036457. 6. Davis, Howard...target cell membranes." Biophysical chemistry 97.2 (2002): 159-172. PubMed PMID: 12050007. 7. Stockschläder...

  6. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...both vectors. Examples of use: Halbert et al., 2002 used an overlapping vector with 440 or 1000bp overlaps...transcripts undergo nonstop decay (Frischmeyer et al., 2002). How to pick a split AAV method While the hybrid...://doi.org/10.1089/hgtb.2015.140 Frischmeyer PA (2002) An mRNA Surveillance Mechanism That Eliminates ...10.1038/sj.mt.6300322 Halbert CL, Allen JM, Miller AD (2002) Efficient mouse airway transduction following recombination...vectors. EMBO Mol Med 6:194–211 . https://doi.org/10.1002/emmm.201302948 Trapani I, Toriello E, de Simone...

  7. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Pallidum 233570 pAAV-AiE0027m_3xC3-minBG-SYFP2-WPRE3-BGHpA AiP14470 AiE0027m_3xC3 SYFP2 Ventromedial hypothalamic...220734 pAAV-AiE0671m_3xC2-minBG-iCre(R297T)-BGHpA AiP20026 AiE0671m_3xC2 Cre Layer 4_IT Isocortex 224043 ...220735 pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA AiP20027 AiE2182m_3xC2 Cre Layer 5_IT Isocortex 214522 ...

  8. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...doi.org/10.3389/fmicb.2015.00242 Pubmed PMID: 25873913 Uraji M, Suzuki K, Yoshida K (2002) A novel plasmid ...Lui et al., 2012, Ni et al., 2008, Uraji et al., 2002). Scientists are beginning to use plasmid incompatibility...:e29875 . https://doi.org/10.1371/journal.pone.0029875 Pubmed PMID: 22253811 Ni B, Du Z, Guo Z, Zhang ...

  9. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...Med Genet 97:147–152 . https://doi.org/10.1002/1096-8628(200022)97:2<147::aid-ajmg6>3.0.co;2-o Baker SA...ameliorates behavioral phenotypes in MeCP2 null (S51.002). Neurology 92 (15 Supplement) . http://n.neurology.org...n.neurology.org/content/92/15_Supplement/S51.002.abstract Sinnamon JR, Kim SY, Fisk JR, Song Z, Nakai H, Jeng...

  10. Western Blot

    Type
    Protocol
    ...Ethanol iBlot 2 PVDF Mini Stack, Thermo Fisher IB24002 20X TBS Tween-20 Nonfat milk powder 96-well microtiter...

  11. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...generated with a transposon-based strategy (Sheridan, 2002). Avoiding protein over-expression artifacts The...-based trafficking." PLoS biology 13.4 (2015): e1002097. PubMed PMID: 25837586. PubMed Central PMCID: ... PubMed PMID: 15247428. PubMed Central PMCID: PMC490022. Shaner, Nathan C., et al. "Improving the photostability...vitro transposition reaction." BMC neuroscience 3.1 (2002): 7. PubMed PMID: 12086589. PubMed Central PMCID...

  12. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...an inducing agent like doxycycline (Favre et al., 2002). They are useful when you need to control the timing..., B., Heard, J.-M., Rivière, Y., & Moullier, P. (2002). Lack of an Immune Response against the Tetracycline-Dependent...11611. https://doi.org/10.1128/JVI.76.22.11605-11611.2002 Griffin, J. M., Fackelmeier, B., Fong, D. M.,...93, 144–157. https://doi.org/10.1016/j.neures.2014.09.002 Zingg, B., Chou, X. lin, Zhang, Z. gang, Mesik...

  13. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    .... 2014 Oct;2(5). doi: 10.1128/microbiolspec.PLAS-0025-2014. PMID: 26104344. Fishel RA, James AA, Kolodner...Electrophoresis. 2010 Jul;31(14):2436-41. doi: 10.1002/elps.201000061. PMID: 20589859. Levene SD. Analysis...cells. J Gene Med. 2009 May;11(5):444-53. doi: 10.1002/jgm.1310. PMID: 19263463. Ribeiro SC, Oliveira ...Biotechnol Bioeng. 2009 Aug 15;103(6):1129-43. doi: 10.1002/bit.22347. PMID: 19408315; PMCID: PMC2735187. More...

  14. Bacterial Expression Systems

    Type
    Collection
    ... cpFRET - FRET-based Biosensors Kit (Addgene #1000000021) is a vector library for the generation of FRET...ratiometric measurements. The MoBiFc Toolkit (Addgene #1000000222) is a collection of Golden Gate compatible vectors...Fluorescence (mCherry) Fusobacterium nucleatum Jörg Vogel 46002 pGR Terminator strength Fluorescence (GFP:mRFP1 ...

  15. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...Sons, Inc. https://doi.org/10.1002/0471140864 Fox, J. D., & Waugh, D. S. (2002). Maltose-Binding Protein...solubility of the tagged protein (Fox and Waugh, 2002). The tag can also be proteolytically cleaved after...

  16. CRISPR Library Amplification

    Type
    Protocol
    ...Micropulser, 0.1 cm ) 20 mL SOC recovery media (Lucigen, 80026-1) 8X LB Agar + Antibiotic 245 mm bioassay plates...

  17. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...cells. Cell Chem Biol. 2025 Jan 21: S2451-9456(25)00002-9. Katharine Diehl Adenosine Express the adenosine...dual-color imaging. PLoS One. 2014 Jun 24;9(6):e100252. Tetsuya Kitaguchi cAMP (cyclic AMP) Red fluorescent...Metabolism in Plants. Plant Cell. 2020 Aug 13. pii: tpc.20.00241. Markus Schwarzländer NADPH BRET semi-synthetic...voltage indicator. Neuron. 2023 Mar 28:S0896-6273(23)00205-2. GENIE Project Voltage Positive-going FRET voltage...

  18. Tips for arabidopsis transformation

    Type
    Blog Post
    ...Arabidopsis are also available (Weigel and Glazebrook, 2002). Find plant plasmid resources at Addgene  Choosing...Agrobacterium strains see Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting...PMID: 17690442. 5. Weigel, D. and Glazebrook, J. (2002) Arabidopsis : a laboratory manual. Cold Spring ...

  19. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...leukemia virus (GALV) are more stable (Sandrin et al., 2002). Studying a viral pathogen more safely. Recently...viruses. Rev Med Virol 28:e1963 . https://doi.org/10.1002/rmv.1963 Mazarakis ND (2001) Rabies virus glycoprotein...Gay W, Nègre D, Le Grand R, Trono D, Cosset F-L (2002) Lentiviral vectors pseudotyped with a modified ...

  20. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...MGG, 152(3), 129–135. https://doi.org/10.1007/BF00268809  Prior, J. E., Lynch, M. D., & Gill, R. T. (2010...Bioengineering, 106(2), 326–332. https://doi.org/10.1002/bit.22695  Schmidhauser, T. J., Ditta, G., & Helinski...Butterworth-Heinemann. https://doi.org/10.1016/B978-0-409-90042-2.50021-0  Toh, W. K., Teo, Y. L., Tor, X. Y., Loh, P. ...
Showing: 1 - 20 of 135 results