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We narrowed to 112 results for: 002

Showing: 1 - 20 of 112 results

  1. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...Microbiol Biotechnol. (2003) 60:523-533 DOI: 10.1007/s00253-002-1158-6 Baker, M., Reproducibility crisis: Blame...

  2. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...Curr Protoc Neurosci. 2020 Dec;94(1):e106. doi: 10.1002/cpns.106. PMID: 33151027; PMCID: PMC7665837. L'...Immunol. 2013 Jul 29;4:217. doi: 10.3389/fimmu.2013.00217. PMID: 23908655; PMCID: PMC3725456. Schwarz H...Biotechnol Bioeng. 2021 Sep;118(9):3499-3510. doi: 10.1002/bit.27776. Epub 2021 May 4. PMID: 33811659. Pace...protein. Protein Sci. 1995 Nov;4(11):2411-23. doi: 10.1002/pro.5560041120. PMID: 8563639; PMCID: PMC2143013...ISSN 1359-0294, https://doi.org/10.1016/j.cocis.2014.10.002. Randolph TW, Schiltz E, Sederstrom D, Steinmann...formation. J Pharm Sci. 2015 Feb;104(2):602-11. doi: 10.1002/jps.24259. Epub 2014 Nov 21. PMID: 25418950; PMCID...

  3. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...reference genes whenever possible (Vandesompele, 2002), and it's possible to multiplex your reference ...issues as a reference gene (Higgins, 2000; Ref, Zhu, 2002), though there are cases in which it is an appropriate...in the literature, a geNorm study (Vandesompele, 2002) will help you select the appropriate reference ...re-optimize protocols for that machine. (Teo, et al, 2002; see paper for more information on optimizing for...Biotechniques. 2000 Aug;29(2):332-7. doi: 10.2144/00292rv02. PMID: 10948434.Taylor SC; Laperriere G;  Germain...0167-7799, https://doi.org/10.1016/j.tibtech.2018.12.002.Teo IA; Choi JW;  Morlese J; Taylor G; Shaun ...target DNA. Journal of Immunological Methods 270 (2002) 119 – 133Tichopad A; Dilger M; Schwarz G; Pfaffl...

  4. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ... thaw cycle (Krajden et al., 1999;  Ugai et al., 2002). If the virus will be used in a couple of days,... two negatively-charged membranes (Davis et al., 2002). Polybrene is one such cationic reagent that is...transduction efficiency by 10-fold (Davis et al., 2002). However, polybrene is highly sensitive to freeze-thaw...delivery." Japanese journal of cancer research 93.5 (2002): 598-603. PubMed PMID: 12036457. 6. Davis, Howard...target cell membranes." Biophysical chemistry 97.2 (2002): 159-172. PubMed PMID: 12050007. 7. Stockschläder...

  5. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...both vectors. Examples of use: Halbert et al., 2002 used an overlapping vector with 440 or 1000bp overlaps...transcripts undergo nonstop decay (Frischmeyer et al., 2002). How to pick a split AAV method While the hybrid...://doi.org/10.1089/hgtb.2015.140 Frischmeyer PA (2002) An mRNA Surveillance Mechanism That Eliminates ...10.1038/sj.mt.6300322 Halbert CL, Allen JM, Miller AD (2002) Efficient mouse airway transduction following recombination...vectors. EMBO Mol Med 6:194–211 . https://doi.org/10.1002/emmm.201302948 Trapani I, Toriello E, de Simone...

  6. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...Med Genet 97:147–152 . https://doi.org/10.1002/1096-8628(200022)97:2<147::aid-ajmg6>3.0.co;2-o Baker SA...ameliorates behavioral phenotypes in MeCP2 null (S51.002). Neurology 92 (15 Supplement) . http://n.neurology.org...n.neurology.org/content/92/15_Supplement/S51.002.abstract Sinnamon JR, Kim SY, Fisk JR, Song Z, Nakai H, Jeng...

  7. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...doi.org/10.3389/fmicb.2015.00242 Pubmed PMID: 25873913 Uraji M, Suzuki K, Yoshida K (2002) A novel plasmid ...Lui et al., 2012, Ni et al., 2008, Uraji et al., 2002). Scientists are beginning to use plasmid incompatibility...:e29875 . https://doi.org/10.1371/journal.pone.0029875 Pubmed PMID: 22253811 Ni B, Du Z, Guo Z, Zhang ...

  8. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...generated with a transposon-based strategy (Sheridan, 2002). Avoiding protein over-expression artifacts The...-based trafficking." PLoS biology 13.4 (2015): e1002097. PubMed PMID: 25837586. PubMed Central PMCID: ... PubMed PMID: 15247428. PubMed Central PMCID: PMC490022. Shaner, Nathan C., et al. "Improving the photostability...vitro transposition reaction." BMC neuroscience 3.1 (2002): 7. PubMed PMID: 12086589. PubMed Central PMCID...

  9. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...an inducing agent like doxycycline (Favre et al., 2002). They are useful when you need to control the timing..., B., Heard, J.-M., Rivière, Y., & Moullier, P. (2002). Lack of an Immune Response against the Tetracycline-Dependent...11611. https://doi.org/10.1128/JVI.76.22.11605-11611.2002 Griffin, J. M., Fackelmeier, B., Fong, D. M.,...93, 144–157. https://doi.org/10.1016/j.neures.2014.09.002 Zingg, B., Chou, X. lin, Zhang, Z. gang, Mesik...

  10. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    .... 2014 Oct;2(5). doi: 10.1128/microbiolspec.PLAS-0025-2014. PMID: 26104344. Fishel RA, James AA, Kolodner...Electrophoresis. 2010 Jul;31(14):2436-41. doi: 10.1002/elps.201000061. PMID: 20589859. Levene SD. Analysis...cells. J Gene Med. 2009 May;11(5):444-53. doi: 10.1002/jgm.1310. PMID: 19263463. Ribeiro SC, Oliveira ...Biotechnol Bioeng. 2009 Aug 15;103(6):1129-43. doi: 10.1002/bit.22347. PMID: 19408315; PMCID: PMC2735187. More...

  11. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...Sons, Inc. https://doi.org/10.1002/0471140864 Fox, J. D., & Waugh, D. S. (2002). Maltose-Binding Protein...solubility of the tagged protein (Fox and Waugh, 2002). The tag can also be proteolytically cleaved after...

  12. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...Arabidopsis are also available (Weigel and Glazebrook, 2002). Find plant plasmid resources at Addgene  Choosing...Agrobacterium strains see Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting...PMID: 17690442. 5. Weigel, D. and Glazebrook, J. (2002) Arabidopsis : a laboratory manual. Cold Spring ...

  13. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...MGG, 152(3), 129–135. https://doi.org/10.1007/BF00268809  Prior, J. E., Lynch, M. D., & Gill, R. T. (2010...Bioengineering, 106(2), 326–332. https://doi.org/10.1002/bit.22695  Schmidhauser, T. J., Ditta, G., & Helinski...Butterworth-Heinemann. https://doi.org/10.1016/B978-0-409-90042-2.50021-0  Toh, W. K., Teo, Y. L., Tor, X. Y., Loh, P. ...

  14. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...leukemia virus (GALV) are more stable (Sandrin et al., 2002). Studying a viral pathogen more safely. Recently...viruses. Rev Med Virol 28:e1963 . https://doi.org/10.1002/rmv.1963 Mazarakis ND (2001) Rabies virus glycoprotein...Gay W, Nègre D, Le Grand R, Trono D, Cosset F-L (2002) Lentiviral vectors pseudotyped with a modified ...

  15. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...to 60% of the cell culture contamination (Uphoff, 2002). Mycoplasmas are considered to be one of the simplest.... J Microbiol .44: 42–49  Uphoff Cc, Drexler Hg (2002) Comparative Antibiotic Eradication of Mycoplasma...Biol Anim 38:86 . https://doi.org/10.1290/1071-2690(2002)038<0086:caeomi>2.0.co;2 Uphoff CC, Drexler HG (...

  16. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...immunology by three papers in the journal Science in 2002. Now, this technique and the in vivo live-cell imaging... PubMed PMID: 18718768. PubMed Central PMCID: PMC2569002. 3. Egen, Jackson G., et al. "Macrophage and ... PubMed PMID: 19578440. PubMed Central PMCID: PMC2700268. 8. Hickman, Heather D., et al. "Direct priming...

  17. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...Angew Chem Int Ed 55:12440–12444 . https://doi.org/10.1002/anie.201606123  Kim JH, Rege M, Valeri J, Dunagin...Angew Chem Int Ed 58:6285–6289 . https://doi.org/10.1002/anie.201900788 Nihongaki Y, Furuhata Y, Otabe T...Angew Chem Int Ed 59:8998–9003 . https://doi.org/10.1002/anie.201914575 Zhou XX, Zou X, Chung HK, Gao Y,...

  18. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...biomedical research. eLife, 13, e100211. https://doi.org/10.7554/eLife.100211...

  19. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23. Pubmed. Find...

  20. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...different human genes encoding GPCRs (Takeda S et al., 2002) and many downstream complexes involved, it can ...Takeda S, Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of G protein-coupled receptor genes...
Showing: 1 - 20 of 112 results