We narrowed to 78 results for: 109
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TypeBlog PostPublishedAug. 31, 2023, 1 p.m.... (EMBC), 4322–4325. https://doi.org/10.1109/EMBC44109.2020.9175600 Park, S., Loke, G., Fink, Y., & Anikeeva...
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Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 10.1073/pnas.1216880109 Resources on Addgene.org CRISPR... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog PostPublishedJune 17, 2025, 1:15 p.m....Acids Research, 50(2), 617–634. https://doi.org/10.1093/nar/gkab1256 Lee, Y., Kim, M., Han, J., Yeom, K...and regulation. Biophysical Reviews, 13(6), 1081–1090. https://doi.org/10.1007/s12551-021-00902-w Additional... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog PostPublishedNov. 7, 2014, 2:56 p.m....lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ- JM109 General cloning and plasmid maintenance, blue/...hsdR514 endA recA1 uidA(∆MluI)::pir-116 Stbl2 JM109-derived. For cloning into and storage of lentiviral... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog PostPublishedSept. 24, 2020, 1:15 p.m....Nucleic Acids Res 44:e141–e141 . https://doi.org/10.1093/nar/gkw583 Rees HA, Liu DR (2018) Base editing:... Acids Research 41:9049–9061 . https://doi.org/10.1093/nar/gkt555 Resources at the Addgene Blog Listen... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog PostPublishedJune 24, 2020, 5:45 p.m....Nucleic Acids Research 47:e23–e23. https://doi.org/10.1093/nar/gky1286 Hilton IB, D’Ippolito AM, Vockley CM... Acids Research 44:5615–5628 . https://doi.org/10.1093/nar/gkw159 Williams RM, Senanayake U, Artibani ... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m.... a thermostable DNA ligase-encoding gene." Gene 109.1 (1991): 1-11. PubMed PMID: 1756968. 7. Lauer, G.... research 10.5 (1982): 1425-1437. PubMed PMID: 7041091. PubMed Central PMCID: PMC320540. 10. Goffin, Colette... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog PostPublishedDec. 17, 2020, 1:30 p.m....Nucleic Acids Research 42:e89–e89 . https://doi.org/10.1093/nar/gku289 Chen C, Fenk LA, de Bono M (2013) Efficient... Acids Research 41:e193–e193 . https://doi.org/10.1093/nar/gkt805 Dickinson DJ, Pani AM, Heppert JK, Higgins... -
Reflections and Looking Ahead
TypeBlog PostPublishedFeb. 25, 2025, 2:15 p.m....Nature Protocols. https://doi.org/10.1038/s41596-024-01095-8 Kahn, R. A., Virk, H., Laflamme, C., Houston, ... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog PostPublishedMay 12, 2015, 1:32 p.m....JJ. Nucleic Acids Res. 2007;35(5):1402-10. doi:10.1093/nar/gkl1108. Epub 2007 Feb 6. PubMed. ... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog PostPublishedAug. 13, 2014, 3:49 p.m....Zakeri et al (Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24. PubMed) ... -
Hot Plasmids - October 2022
TypeBlog PostPublishedOct. 6, 2022, 1:15 p.m....–974 (2022). https://doi.org/10.1038/s41593-022-01094-6. (This work was also shared as a preprint: https... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog PostPublishedJune 27, 2018, 1:16 p.m....plasmid here! Zhao C, et al. RNA. 2018 PubMed PMID:29109157. Nanobody toolkit to monitor retrograde transport... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog PostPublishedFeb. 14, 2018, 2:36 p.m....and Eric A. Hendrickson. Genome Res 27(7) (2017):1099-1111. PubMed PMID: 28356322 PubMed Central PMCID... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....imaging." BMC biology 6.1 (2008): 13. PubMed PMID: 18325109. PubMed Central PMCID: PMC2292683. Shaner, Nathan... -
What the HEK?
TypeBlog PostPublishedApril 18, 2023, 1:15 p.m....HEK 293 cells. FASEB J. (2002) (8):869-71. DOI: 10.1096/fj.01-0995fje Tan, E., et al., HEK293 Cell Line... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m....developed (mNeptune684 with C65S, T107K, A108V, T109K, H161Y, P163T, I175Q, C176T and N177F mutations)... -
Antibodies 101: Monoclonal Antibodies
TypeBlog PostPublishedJune 29, 2021, 1:15 p.m....Resources. ILAR Journal 46:258–268. https://doi.org/10.1093/ilar.46.3.258 Mould AP, Askari JA, Byron A, Takada... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog PostPublishedMay 3, 2018, 1:48 p.m....WYL-Domain-Containing Accessory Protein.” Mol Cell. (2018) pii: S1097-2765(18)30173-4. PubMed PMID: 29551514 Plasmids... -
Viral Vectors 101: Types of viruses
TypeBlog PostPublishedJune 6, 2023, 1:15 p.m....Rev Infect Dis. 1982 Sep-Oct;4(5):1015-25. DOI: 10.1093/clinids/4.5.1015 Lee C.S., et al. Adenovirus-Mediated...