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We narrowed to 79 results for: 109

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Showing: 21 - 40 of 79 results

  1. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ... Acids Research 41:7429–7437 . https://doi.org/10.1093/nar/gkt520 Citorik RJ, Mimee M, Lu TK (2014) Sequence-specific...Biochimica et Biophysica Sinica. https://doi.org/10.1093/abbs/gmab036 Liu Y, Wan X, Wang B (2019) Engineered...Nucleic Acids Res 44:e143–e143 . https://doi.org/10.1093/nar/gkw625 Resources on the Addgene Blog Check...

  3. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: https://doi.org/10.1073/pnas.1216880109  Resources on Addgene.org...

  4. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...Acids Research, 50(2), 617–634. https://doi.org/10.1093/nar/gkab1256 Lee, Y., Kim, M., Han, J., Yeom, K...and regulation. Biophysical Reviews, 13(6), 1081–1090. https://doi.org/10.1007/s12551-021-00902-w Additional...

  5. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ- JM109   General cloning and plasmid maintenance, blue/...hsdR514 endA recA1 uidA(∆MluI)::pir-116  Stbl2   JM109-derived. For cloning into and storage of lentiviral...

  6. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...Nucleic Acids Research 47:e23–e23. https://doi.org/10.1093/nar/gky1286 Hilton IB, D’Ippolito AM, Vockley CM... Acids Research 44:5615–5628 . https://doi.org/10.1093/nar/gkw159 Williams RM, Senanayake U, Artibani ...

  7. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ... a thermostable DNA ligase-encoding gene." Gene 109.1 (1991): 1-11. PubMed PMID: 1756968. 7. Lauer, G.... research 10.5 (1982): 1425-1437. PubMed PMID: 7041091. PubMed Central PMCID: PMC320540. 10. Goffin, Colette...

  8. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...Nucleic Acids Research 42:e89–e89 . https://doi.org/10.1093/nar/gku289 Chen C, Fenk LA, de Bono M (2013) Efficient... Acids Research 41:e193–e193 . https://doi.org/10.1093/nar/gkt805 Dickinson DJ, Pani AM, Heppert JK, Higgins...

  9. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...Nucleic Acids Res 44:e141–e141 . https://doi.org/10.1093/nar/gkw583 Rees HA, Liu DR (2018) Base editing:... Acids Research 41:9049–9061 . https://doi.org/10.1093/nar/gkt555 Resources at the Addgene Blog Listen...

  10. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...Nature Protocols. https://doi.org/10.1038/s41596-024-01095-8 Kahn, R. A., Virk, H., Laflamme, C., Houston, ...

  13. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...–974 (2022). https://doi.org/10.1038/s41593-022-01094-6. (This work was also shared as a preprint: https...

  18. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...HEK 293 cells. FASEB J. (2002) (8):869-71. DOI: 10.1096/fj.01-0995fje Tan, E., et al., HEK293 Cell Line...

  19. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...Resources. ILAR Journal 46:258–268. https://doi.org/10.1093/ilar.46.3.258  Mould AP, Askari JA, Byron A, Takada...
Showing: 21 - 40 of 79 results