Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 149 results for: Coli

Showing: 21 - 40 of 149 results

  1. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...cyanobacteria, synthetic inverters in E. coli, and biosensors in E. coli: Čelešnik, Helena, et al. "Biosafety...synthetic biology or protein purification studies in E. coli. 800 and 1900 Series If you’re expressing two genes... be used to precisely tune your polycistronic E. coli expression constructs. Some applications of plasmids...in the kit can be used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending...aggregation propensity in four expression systems: E. coli, wheat germ, HeLa, and Leishmania (Gagoski, et al... clone your segments of choice into either an E. coli or a mammalian expression vector in a one-pot reaction...

  2. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...interaction detection (RaPID) method. RaPID borrows the E. coli biotin ligase BirA* from BioID and allows a researcher...interactions. Both methods rely on the promiscuous E. coli biotin ligase, BirA*, to biotinylate proteins that...subtilis. BASU was  compared head-to-head with the E. coli and BioID2 BirA*s in the context of RaPID for their...no detectable signal at the same time point. E. coli’s BirA* was tested at its optimum 18 hour time point... generated a >30-fold enrichment compared to E. coli’s BirA*. Faster labeling kinetics also means that...

  3. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...some E. coli strains have natural antibiotic resistances, so make sure your plasmid and E. coli strain ... makes our lives. Plasmid transformation into E. coli is a fairly inefficient process– just 1 out of 10,000...are compatible! Check out this list of common E. coli genotypes and their natural resistances.  Additional...

  4. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...highest levels of nonlinear acoustic contrast in E. coli. We deleted an unannotated hypothetical protein,... with several inducible expression systems in E. coli. The arabinose-inducible promoter pBAD and a low-copy...pBAD-bARGSer-AxeTxe, gas vesicle expression in E. coli is easy: simply grow cells in Lennox LB (10 g/L ...expression in vivo is also straightforward: after the E. coli have colonized tumors, inject L-arabinose intraperitoneally...).     Fig. 1: Expressing bARGSer in E. coli. (a) Plasmid diagram of pBAD-bARGSer-AxeTxe. (b)...imaging of tumor colonization by bARGSer-expressing E. coli. The GV-specific ultrasound signal inside the tumors...

  5. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    Published
    May 24, 2018, 1:20 p.m.
    ...dsRNA-expressing E. coli that are mixed with liver paste. Prior to mixing with the liver paste, these E. coli are transformed...the  induction of T7 polymerase encoded in the E. coli genome (usually controlled by IPTG). For this IPTG-induced...expression to work, students have to use a commercial E. coli strain that is designated as DE3. For Phagocata ...eaters and refuse to eat liver paste mixed with E. coli. Thus, to get the dsRNA into Phagocata morgani and...

  6. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...Check the bacterial strain Differences between E. coli host strains can impact plasmid yield. Check the...is advisable to check out the genotype of your E. coli to ensure it is suitable for your application. We... We have previously reviewed many common lab E. coli strains on the blog, and also recommend this handy...OpenWetWare for a more extensive listing of common E. coli strains. Increase the volume Starting with a larger...to Verify Your Plasmid Learn about Common Lab E. coli Strains Additional Resources on Addgene.org Find...

  7. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...protocol) At Addgene, we use the Mix & Go! E. coli Transformation Kit and Buffer Set  from Zymo Research...available via Zymo Research. In brief, we grow our E. coli in LB to log phase, then wash and resuspend the ... protocol has worked well for us for multiple E. coli strains, including DH5alpha, XL1 Blue, TOP10, ccdB...

  8. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...response to changing pH, and they can rupture E. coli spheroplasts to release encapsulated proteins. Video...in a single operon and were first expressed in E. coli in the 1980s  (Kanabrocki et al 1986). Much later...showed that these fluorescent R bodies can rupture E. coli spheroplasts (cells which have had their cell walls...Absence of toxicity of R-body producing recombinant E. coli on paramecia. European Journal of Protistology 48...

  9. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...plasmids suitable for other model systems, including E. coli, plants, Drosophila, and zebrafish!   Why use multiplexed...Assembly or SLIC cloning methods. A BsaI-based E. coli multiplexing plasmid is available from the Koffas...supplied on a separate plasmid. Multiplexing in E. coli Koffas Lab CRISPathBrick Multiplex Plasmid: This...arrays A, B, C, AB, AC, BC, and ABC). Nielsen Lab E. coli CRMAGE Plasmids: The CRMAGE system is a fast, multiplexable...incubation. Kondo Lab multiplexed base editing in E. coli The Kondo lab expressed a cytidine deaminase fusion...LVA tag) to achieve specific point mutations in E. coli. gRNAs are delivered on a different plasmid and ...Mediated Multiplex Transcriptional Repression in E. coli.” ACS Synthetic Biology 4(9) (2015):987-1000. PubMed...

  10. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...looking at an example plasmid, let's review how E. coli normally replicate their chromosomal DNA. Chromosomal...positive and negative regulators. The genome of E. coli is about 4.6 million base pairs long and contains...re-activate DnaA. These regulatory mechanisms allow E. coli to control chromosomal replication and balance population...concentration of plasmid. Similar to replication in E. coli, regulation primarily occurs by controlling the ...controlled and has a low copy number. Like the E. coli chromosome, it has a single ori and requires the...regulate plasmid replication. For example, the plasmid ColIb-P9 encodes the protein RepZ to drive its own replication...An antisense RNA called Inc, also encoded by the ColIb-P9 plasmid, is complementary to part of the sequence...

  11. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...these broad host range plasmids all replicate in E. coli, which is the most commonly used bacteria in lab...Erwinia carotovora, E. chrysanthemi, Escherichia coli, Gluconacetobacter xylinus, Gluconobacter spp., ...herbicola, E. rubrifaciens, E. stewartii, Escherichia coli, Klebsiella spp., Legionella pneumophila, Methylophilus...Brucella spp., Caulobacter crescentus, Escherichia coli, Gluconacetobacter xylinus, Paracoccous denitrificans...promoter-based expression systems for Escherichia coli and Agrobacterium tumefaciens. 3 Biotech, 13(3),... ferrooxidans, Vibrio salmonicida, Yersinia enterocolitica, Xanthomonas campestris, X. maltophilia ...

  12. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...glycosylated proteins that cannot be produced in  E. coli or yeast cells. The only problem with baculovirus...bacterial host cell is almost always the answer. E. coli is definitely one of the most popular hosts for ...expression may be required if your protein is toxic to E. coli. Of all the vectors, pET, under the control of the...Systems Recombinant protein expression in Escherichia coli: advances and challenges Production of recombinant...

  13. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...knockout E. coli strain to assay for functional decaffeination in cells. The ΔguaB E. coli are missing... CBB5 and has been tailored for expression in E. coli. This operon is under the transcriptional control...Janthinobacterium sp. Moreover, a caffeine “addicted” E. coli strain expressing this operon can serve as a living...

  14. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...Lederberg in 1952 was noted to integrate into the E. coli chromosome in some cases. This terminology held ... plasmid, pSC102, with EcoRI and selected for E. coli transformants that were resistant to both. When ... M. Lederberg. “Sex Compatibility in Escherichia Coli.” Genetics 37.6 (1952): 720–730. PubMed PMID: 17247418...

  15. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ... of eight bacterial toxin genes isolated from E. coli or Psuedomonas aeruginosa. These toxins were a mix...Gram-negative Enterobacteriaceae including in E. coli O157:H7, Shigella flexneri, Enterobacter cloacae... et al. "Toxic protein expression in Escherichia coli using a rhamnose-based tightly regulated and tunable...simplified chromosomal gene replacement in Escherichia coli." Appl. Environ. Microbiol. 81.15 (2015): 5103-5114...Recombineering) system for genome editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed PMID...

  16. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...delight,” the very first protein they tested — E. coli expressing good ol’ EGFP — showed a slight but detectable...: Fluorescence intensity of EGFP-FlavinTag in E. coli changes in response to a ~25 mT magnet. Gray regions...magnetoresponse was clearly visible whether expressed in E. coli, in mammalian cells, or in purified form, and didn...license.   Video 1: Fluorescence movie of E. coli expressing MagLOV while a magnet is waved under ...

  17. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...performed, the next step is to transform competent E. coli cells and select the positive clones. The entry ...expression clone. You will also need to use a E. coli strain sensitive to CcdB (e.g. DH5α, TOP10, Mach1...protein inhibits the growth of CcdB sensitive E. coli strains, most colonies should contain the desired...available for all kind of organisms from mammals to E. coli. Glossary of Gateway cloning vectors Vector type...

  18. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...into E. coli and plasmid assembly (re-circularization) occurs in vivo. We use XL-10 Gold E. coli, but have...plasmid in vitro, before transforming it into E. coli for propagation. However, while not widely known...successful recombination reported in all laboratory E. coli strains tested so far. The use of this pathway as...

  19. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...remain the lovely whitish-cream color of standard E. coli. On the other hand, intact β-galactosidase produces...restriction digest. Make sure you use a proper E. coli strain (i.e. contains the lacZΔM15 mutation): XL1...the Addgene Blog Read Our Post on Common Lab E. coli Strains Learn to Use Yeast Two Hybrid Systems Browse...

  20. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...the late nineties, this was developed into an E. coli system, with the noncanonical amino acid in the ...expression of phosphoserine-including proteins in E. coli (Zhu et al., 2022). It uses the TAG codon for the...Phosphoserine into Recombinant Proteins in Escherichia coli. 12(21). https://doi.org/10.21769/BioProtoc.4541...
Showing: 21 - 40 of 149 results