...changes one gene at a time. CRISPR-based systems enable gene knockout in immune cells in a high-throughput...developed CHIME (CHimeric IMmune Editing system) to enable the constitutive, ubiquitous knockout of single...would be quite useful for interrogating these scenarios. X-CHIME We recently developed and deposited four... four new systems to fill this gap. To enable context-specific knockouts, we developed X-CHIME, a suite...Doench, J. G., & Sharpe, A. H. (2024). X-CHIME enables combinatorial, inducible, lineage-specific and ...

...amplifies the intensity of fluorescence signal and enables tracking of single molecules within living cells...transcriptional activation domains. This system enabled the specific recruitment of multiple copies of ...peptide with the light-sensitive LOV2 domain of Avena Sativa phototropin 1 (AsLOV2). In the dark, this...screened plasmids contain a gene variant* and is amenable to both high and low throughput screening applications...recently developed a CRISPR-based multicolor tool enabling the imaging of multiple endogenous genomic loci...re-assigned stop codon (TAG). This polyspecificity enables the site-specific introduction of many different...pCell-Free vectors, Gateway-compatible backbones which enable cell-free expression of proteins in both prokaryotic...

...Throughout their history, fluorescent dyes have enabled the visualization of both organic tissues and cell...of the characteristics of these fluorophores to enable highly specific visualization of cellular components...495/517 nm. FITC binds to an amine on a protein, enabling the labeling of protein substrates/peptides and...impacted by several factors. Firstly, brighter dyes enable detection of finer details by improving signal-...STORM). These dyes have specific properties that enable resolution beyond the diffraction limit of light... compatible excitation and emission profiles to enable effective energy transfer, which is key to observing...

... "black box" provided by the manufacturers and enables customized experiments. Projects such as the OpenFlexure...Raspberry Pi, the Arduino, or 3D printers are key to enabling this work. Sharing open-source plans that rely.... These newly developed techniques are the key enablers for making assemblies open-source. The sharing... to accelerate scientific progress, but also to enable science in parts of the world that are otherwise... PhD from the Heintzmann Lab at the Leibniz IPHT Jena. He is co-founder of the open-hardware startup “...

...Zhang lab for GeCKO, a lentiviral CRISPR library enabling genome-scale, knockout screening). As the Zhang...pooled CRISPR libraries, also available at Addgene, enabling systematic investigation of the cellular consequences...with minimal off-target effects. The system also enabled them to turn on genes anywhere in the genome for... now. They are also developing sgRNAs that will enable even more fine-tuning of gene expression levels...Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H,...

...and spun down the homogenate to remove the insoluble material. Then, the homogenate was loaded onto cesium-chloride...effectively isolate intact RNA, a fast, strong protein denaturant would be required- something that broke down ...Chirgwin and colleagues showed that a strong protein denaturant, guanidinium thiocyanate, did just this (Chirgwin...sodium acetate, and chloroform are added to the homogenate and shaken. After a quick centrifugation (not...

...disrupted by denaturants (Hochuli, 1988). In some cases, proteins can be bound under denaturing conditions...gradual removal of the denaturant. Not all proteins will refold once denatured, however, so take care ...neither placement works, consider purifying under denaturing conditions. Unlike other affinity purification...

...developed another useful optogenetic tool that enables robust and efficient oligomerization of target ...additional 9 amino acid peptide, the CRY2clust domain enables fast and reversible oligomerization when fused ...upon termination of light stimulation. CRY2clust enables faster assembly and disassembly dynamics, higher... PMID: 28646204. soCoChR: A novel opsin that enables single-cell optogenetics Article contributed by...IS5 plasmid containing a synthetic operon that enables the degradation of caffeine (1,3,7-trimethylxanthine...

...lentiviral CRISPR library     CRISPR pooled libraries enable researchers to screen the entire genome for genes...way scientists create new traits in bacteria and enabled the in-depth study of many biological processes...only genome engineering method in bacteria that enables rapid, automated, and high-throughput genome editing...temperature-controlled expression of the MutL mutant enables transient suppression of DNA repair during oligonucleotide...developed CRISPR tools for epigenome editing that enable the precise study of particular epigenetic modifications....1-GoldenGate, pcDNA3.1-GoldenGate-VP64 Maria-Elena Torres-Padilla pTALYM3, pTALYM4 Boris Greber pTAL7a... drug-resistance. Remarkably, this method also enabled Lourido’s group to define ~200 previously uncharacterized...

... long-term use is likely to require on-going maintenance and support.” Besides that, academic institutions... that an ELN supports all types of data, which enables researchers to store everything in one place. From...The values of ELNs show in the long run as they enable researchers to keep all their data in one place... and assign them roles and permission that only enable them to see the necessary information. Does your... to implement an ELN, but on the long run, ELNs enable labs to keep their data in one place and therefore...

...plasmid grant awardees: Drs Louis Bengyella and Kwabena O. Duedu, both from the University of Health and... women’s health in Ghana and around the world. Kwabena O. Duedu Dr. Duedu’s molecular biology work has...and tools to construct chimeric cellulases that enable E. coli and Citrobacter freundii to generate sugars...Addgene community and hope we can do even more to enable scientists in developing regions in the near future...

...with the correct wavelength, microbial rhodopsins enable ions to flow across the axon’s membrane, thereby...Figure 2) (Sharma and Zhu, 2014). How does FLEx enable strong opsin expression in specific neuronal cells... of the double-floxed opsin construct, thereby enabling its expression under a strong promoter only in...transgenic mice targeting different brain areas enabled the drawing of a detailed and functional topography...21.5 (2003): 562. PubMed PMID: 12665802. SSharma, Suveena, and Jinfang Zhu. "Immunologic applications of ...

...interest. If you are working with cells that are amenable to genetic knockout, this represents a very robust...essential to the cell’s function, it likely is not amenable to knockout approaches. In these cases, we would...be difficult to achieve sufficient knockdown to enable robust assessment of antibody selectivity. Additionally...). Scaling of an antibody validation procedure enables quantification of antibody performance in major...Schwartz, J., Kolahdouzan, M., Chen, C. X., You, Z., Benaliouad, F., Gileadi, O., McBride, H. M., Durcan, T. ...

...libraries are popular tools for genetic screens, enabling genome-wide interrogation of the genes required...lentiviral systems for mammalian screens, which enables researchers to maximize the chance of transducing...are refractory to lentiviral transduction, now enabling forward genetic screens in cells with few genetic...active in E. coli and closely related species, is amenable to use with other bEBP/UAS pairs, and displays...

...called CAST (CRISPR-associated Transposase) both enable gRNA-directed transposition. INTEGRATE consists...organism/gene interactions with the host. This scenario allows for several potential CRISPR applications...utility of cytidine and adenine base editors that enable C-to-T and A-to-G base substitutions respectively...Liu Y, Wan X, Wang B (2019) Engineered CRISPRa enables programmable eukaryote-like gene activation in ...RJ, Grossman AD, Gross CA, Rosenberg OS (2019) Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi...

...Kim, J.-Y. (2024). Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis... only was ASAP5 able to detect mEPSPs, it also enabled imaging of their propagation along dendrites for...represent the largest family of human drug targets. To enable large-scale profiling of many GPCRs, Mikel Garcia-Marcos... functional heterotrimers with endogenous Gβγ, enabling detection of activation triggered by endogenous...

...Sainsbury has a mandate to share and educate and enable plant science in general. We are funded by a charity...validating them, it made the most sense to us to enable other people to do that too. We don’t have the ... also. There is also a U.K. organization called Athena SWAN that is a charter for advancing women in science...positions. There has been a lot of engagement with the Athena SWAN Charter and it has really strengthened the...

...nervous system (CNS) orchestrates complex processes enabling organisms to control their movements and behavior...create an atlas of the mouse brain. This atlas enables researchers to quickly find the best AAV serotypes...it has been crucial to develop new tracers that enable accurate labeling of neurons. As discussed above...fidelity and at lower concentrations than GFP. smFPs enable better labeling of these fine structures through...

...instead of on the yeast extracellular surface) which enables tracking of protease activity with a fluorescent...electroporation and AAV donor infection (CRISPR-READI) enables genome engineering using HDR donors lengths up ...at Addgene! A new dual-deaminase base editor enables concurrent adenine and cytosine editing. Find ...optimized for excitation wavelengths above 1,000 nm and enable two-color imaging with red fluorescence protein-based...

...success of zebrafish as a model organism is its amenability to genetic manipulation. Tools for gene manipulation...). “Brainbow” fish lines have been developed to enable high-resolution mapping of the brain by uniquely...uniquely labeling neurons. Zebrafish are also amenable to manipulation of brain activity: optogenetic approaches...three-month generation time and labor-intensive maintenance compared to invertebrate model systems, and their...